Development of a genus specific primer set for detection of<i>Leishmania</i>parasites by polymerase chain reaction

Bhattacharyya, Riddhi; Das, Krishna; Sen, Suvajit; Roy, Syamal; Majumder, Hemanta K.

doi:10.1111/j.1574-6968.1996.tb07989.x

Cited by 24 publications

(3 citation statements)

References 8 publications

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“…In addition, diagnostic studies have gained momentum. The diagnosis of leishmaniasis has been approved by isolating, visualizing, and culturing the parasite from infected tissue, historically [104]. It explains that there is a serious need to develop more expensive, precise, sensitive, rapid, and specific diagnostic tests, especially for the detection of leishmaniasis [105].…”

Section: Discussionmentioning

confidence: 99%

Modern Aspects of Leishmaniasis: Basis of Development New Approaches against Infection

Pinar Zorba Yildiz,

Yildirim Koken,

Yavuz

et al. 2024

Current Topics in Zoonoses

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In this review, the basic principles of developing new approaches to leishmaniasis have been stated contrary to the available literature on Leishmania. In general, the morphology of parasites, life cycle, clinical forms, correspondence to epidemiology, and distribution according to species in the world were described. It has been expressed in various aspects of the interaction between host and parasite cell receptors. This plays an extremely important role in vaccine and drug development against leishmaniasis. Immunologically, natural immunocytes emphasize the importance of macrophages and dendritic cells in the Leishmania parasites’ immunopathology. This review will also discourse on the possibilities and necessity for the generation of new treatment technologies for leishmaniasis.

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Section: Discussionmentioning

confidence: 99%

Modern Aspects of Leishmaniasis: Basis of Development New Approaches against Infection

Pinar Zorba Yildiz,

Yildirim Koken,

Yavuz

et al. 2024

Current Topics in Zoonoses

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“…In the current study, we tried to detect Leishmania species using several PCR assays and compared the sensitivity and specificity of these methods. Generally, the use of highly repetitive genomic loci or extrachromosomal kDNA sequences is the most common method, which is confined to the detection of parasites at the genus or complex levels ( 18 ). The present study showed that, while PCR based on minicircle kDNA sequences could correctly detect the positive samples, it is not a good tool to differentiate and identify the Leishmania isolates.…”

Section: Discussionmentioning

confidence: 99%

The Accuracy of Diagnosis and Genotyping of Leishmania Species Based on Spliced Leader Mini-Exon Gene by Nuclear Magnetic Resonance and Sequencing Assays

Khorram,

Masjedi,

Tabrizi

et al. 2023

IJPA

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Background: We aimed to evaluate the accuracy of genotyping of Leishmania species by the spliced leader mini-exon gene. Methods: Suspected leishmaniasis patients, referred to Masieh Daneshvary Hospital, Tehran, Iran were included from May 2017 to September 2021. The Leishmania species were genotyped by PCR-RFLP based on the SL mini-exon gene and the ITS1 region of SSU-rRNA gene and compared with the sequencing results. The expressed metabolites of metacyclic promastigotes were evaluated by Proton nuclear magnetic resonance (1H-NMR). Results: Out of 66 suspected cases, 36 (54.4%) were positive for Leishmania species based on the PCR assays. In 21 (31.8%) cases, promastigotes grew on culture tubes. Based on the RFLP of SL RNA profile, 13 (19.7%) L. tropica, 9 (13.6%) L. major, 3 (4.5%) L. infantum, and 8 (12.1%) C. fasciculata isolates, isolated from culture media, were identified; however, 3 (4.5%) cases were unidentifiable due to the low number of parasites. Seventeen metabolites were expressed by the metacyclic forms of L. major, L. tropica and C. fasciculata isolates. The top differential metabolites expressed more in C. fasciculata were FAD, p-Methoxybenzyl alcohol and S-b-G-5, 5-G-b-S (A = CH2) (P<0.005) whereas Veratryl glycerols and D-(+)-Mannose were significantly increased in L. major and Betulin, L-Tyrosine in L. tropica (P<0.01). Conclusion: The invaluable techniques such as sequencing and 1H-NMR confirmed the results of genotyping of Leishmania species based on the SL mini-exon gene. SL mini exon gene can be used as a diagnostic tool to differentiate various Leishmania genotypes and detect contamination of culture media with C. fasciculata.

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“…For example, bile exposure in E. coli increases expression of multidrug-efflux pumps including AcrAB and EmrAB that promote bile tolerance and increase antibiotic efflux [35, 36]. In S. typhimurium , an AcrB-like protein that encodes for an efflux pump contributes to resistance to both bile salts and detergents [37, 38]. Bile exposure in Enterococcus faecalis induces genes encoding for EmrB/QacA family drug resistance transporters and increases expression of phospholipid synthetase which is linked to daptomycin resistance [39].…”

Section: Introductionmentioning

confidence: 99%

Impact of Remote Cholecystectomy on Clinical Outcomes FollowingPseudomonas aeruginosaBloodstream Infection: A Retrospective Cohort Study

Bertucci,

Heise,

Angarone

et al. 2024

Preprint

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Purpose: Mortality associated withPseudomonas aeruginosabloodstream infection (PABSI) remains high despite advances in clinical care and therapeutics. In a recent study using mice to model PABSI, the gallbladder (GB) was identified as a reservoir for bacterial expansion. Furthermore, bile exposure has been linked to increased antimicrobial resistance (AMR). Therefore, we asked whether patients with retained gallbladders might suffer from more antimicrobial resistantP. aeruginosa(Pa) infections, extended culture positivity, and worsened clinical outcomes. Methods: Retrospective cohort study of adults hospitalized over a five-year period with PABSI. PABSI cases were defined as patients with ≥ 1 positivePabacterial culture from the blood. Patients were categorized as either those retaining a gallbladder (no cholecystectomy) or those without (cholecystectomy). Cholecystectomy was defined as a history of cholecystectomy ≥ 1 year prior to the index episode of PABSI. Inferential statistics were used to identify associations between remote cholecystectomy and antimicrobial resistance profile, length of blood culture positivity, and in-hospital and 90-day mortality. Results: The final study population included 336 patients, 262 (78%) with retained gallbladder and 74 (22%) without. We observed no difference in 90-day or in-hospital mortality between groups. Overall, composite 90-day mortality was 30.1%. Furthermore, no robust differences were observed in the antimicrobial resistance profile of Pa isolates between the groups. Conclusions: In our study, neither PABSI AMR pattern nor clinical outcome was affected by remote cholecystectomy. However, we do demonstrate that mortality for patients with PABSI in the modern era remains high despite advances in anti-pseudomonal therapeutics.

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Development of a genus specific primer set for detection ofLeishmaniaparasites by polymerase chain reaction

Cited by 24 publications

References 8 publications

Modern Aspects of Leishmaniasis: Basis of Development New Approaches against Infection

Modern Aspects of Leishmaniasis: Basis of Development New Approaches against Infection

The Accuracy of Diagnosis and Genotyping of Leishmania Species Based on Spliced Leader Mini-Exon Gene by Nuclear Magnetic Resonance and Sequencing Assays

Impact of Remote Cholecystectomy on Clinical Outcomes FollowingPseudomonas aeruginosaBloodstream Infection: A Retrospective Cohort Study

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