“…The functionality of the CRISPR/Cas9 system as a genome editing method has been reported in filamentous fungi (Arazoe et al, 2015;Fuller et al, 2015;Katayama et al, 2016;Liu et al, 2015;Nødvig et al, 2015). We recently developed the CRISPR/Cas9 system as a genome editing method in A. oryzae and demonstrated that the system can be applied to industrial strains (Katayama et al, 2016). However, the efficiency of mutagenesis using the CRISPR/Cas9 system was 10~20% (Katayama et al, 2016), which is lower than the gene targeting efficiencies in the deletion of ku70 and ligD (Maruyama and Kitamoto, 2008;Mizutani et al, 2008;Takahashi et al, 2006).…”