Development of a genetically modified bacteriophage for use in tracing sources of pollution

Daniell, Tim J.; Davy, M.L.; Smith, Richard J.

doi:10.1046/j.1365-2672.2000.01028.x

Cited by 7 publications

(3 citation statements)

References 20 publications

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“…cloacae phage even in microbial‐rich waters and satisfactory recovery at low flow rates has revealed this biotracer to be the most promising at this stage. Unlike a recent hydrological study (Daniell et al. 2000) this research has revealed that genetically modified biotracers are not required.…”

Section: Discussionmentioning

confidence: 90%

“…cloacae phage even in microbial-rich waters and satisfactory recovery at low flow rates has revealed this biotracer to be the most promising at this stage. Unlike a recent hydrological study (Daniell et al 2000) this research has revealed that genetically modified biotracers are not required. However, further seasonal field studies 'especially at high flow rates' are necessary to provide a thorough evaluation for each biotracer.…”

Section: Discussionmentioning

confidence: 90%

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Evaluation of biotracers to monitor effluent retention time in constructed wetlands

Hodgson

Perkins

Labadz

2003

Letters in Applied Microbiology

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Aims: With concern surrounding the environmental impact of chemical tracers on the aquatic environment, this paper presents the initial evaluation of biotracers used to determine the effluent retention time, an important performance indicator, in a Free Water Surface Constructed Wetland. Methods and Results: Production of the biotracers, coliphage MS2, and the bacteriophage of Enterobacter cloacae and antibiotic resistant endospores of Bacillus globigii is described in detail. Their subsequent use in three separate tracer experiments -January, March and June (2000) -revealed the variability of retention time with respect to effluent flow. The biotracer MS2 showed the constructed wetland had a retention time of 8-9 h at a mean discharge of 0AE9 l s )1 , increasing to 10-12 h at a mean discharge 0AE3 l s )1 . A similar retention of 9-10 h at a mean discharge of 0AE3 l s )1 was calculated for the Ent. cloacae phage. In contrast, use of endospores revealed considerably longer retention times at these mean discharge rates; 12-24 h and 36-48 h, respectively. Conclusion: Biotracers could provide a useful and environmentally friendly technique to monitor effluent retention in constructed wetlands. At this stage the phage tracers appear particularly promising due to ease of isolation and recovery. Significance and Impact of the Study: Initial results are encouraging and have highlighted the potential of biotracers as alternatives to chemical tracers, even in microbially-rich waters.

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Section: Discussionmentioning

confidence: 90%

Section: Discussionmentioning

confidence: 90%

Evaluation of biotracers to monitor effluent retention time in constructed wetlands

Hodgson

Perkins

Labadz

2003

Letters in Applied Microbiology

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“…Using suitably modified filamentous phages along with other biocontrol agents can revolutionize the integrated management of phytopathogens. However, these phages are at times unstable in their host populations, even when they do not result in the evolution of host resistance (Lerner & Model, 1981 Daniell, Davy, and Smith (2000) affect members of Ralstonia are useful in controlling phytopathogenic strains of the bacteria and in improving the efficacy of endophytic bacterial strains used in phytoremediation.…”

Section: Phages For Biological Controlmentioning

confidence: 99%

Application of filamentous phages in environment: A tectonic shift in the science and practice of ecorestoration

Sharma

Karmakar

Kumar

et al. 2019

Ecology and Evolution

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Theories in soil biology, such as plant–microbe interactions and microbial cooperation and antagonism, have guided the practice of ecological restoration (ecorestoration). Below‐ground biodiversity (bacteria, fungi, invertebrates, etc.) influences the development of above‐ground biodiversity (vegetation structure). The role of rhizosphere bacteria in plant growth has been largely investigated but the role of phages (bacterial viruses) has received a little attention. Below the ground, phages govern the ecology and evolution of microbial communities by affecting genetic diversity, host fitness, population dynamics, community composition, and nutrient cycling. However, few restoration efforts take into account the interactions between bacteria and phages. Unlike other phages, filamentous phages are highly specific, nonlethal, and influence host fitness in several ways, which make them useful as target bacterial inocula. Also, the ease with which filamentous phages can be genetically manipulated to express a desired peptide to track and control pathogens and contaminants makes them useful in biosensing. Based on ecology and biology of filamentous phages, we developed a hypothesis on the application of phages in environment to derive benefits at different levels of biological organization ranging from individual bacteria to ecosystem for ecorestoration. We examined the potential applications of filamentous phages in improving bacterial inocula to restore vegetation and to monitor changes in habitat during ecorestoration and, based on our results, recommend a reorientation of the existing framework of using microbial inocula for such restoration and monitoring. Because bacterial inocula and biomonitoring tools based on filamentous phages are likely to prove useful in developing cost‐effective methods of restoring vegetation, we propose that filamentous phages be incorporated into nature‐based restoration efforts and that the tripartite relationship between phages, bacteria, and plants be explored further. Possible impacts of filamentous phages on native microflora are discussed and future areas of research are suggested to preclude any potential risks associated with such an approach.

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References

2010

Wastewater Microbiology

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Development of a genetically modified bacteriophage for use in tracing sources of pollution

Cited by 7 publications

References 20 publications

Evaluation of biotracers to monitor effluent retention time in constructed wetlands

Evaluation of biotracers to monitor effluent retention time in constructed wetlands

Application of filamentous phages in environment: A tectonic shift in the science and practice of ecorestoration

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