Development of a genetic sexing strain of <i>Anopheles arabiensis</i> for <scp>K</scp>wa<scp>Z</scp>ulu‐<scp>N</scp>atal, <scp>S</scp>outh <scp>A</scp>frica

Dandalo, Leonard; Munhenga, Givemore; Kaiser, Maria; Koekemoer, Lizette L.

doi:10.1111/mve.12264

Cited by 12 publications

(17 citation statements)

References 24 publications

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“…For this reason, ongoing programmes targeting African malaria vectors focus only on small and/or ecologicallyisolated populations such as An. arabiensis in Northern Sudan or KwaZulu, Natal in South Africa [110][111][112][113]. These projects are in their developmental stages and have generated the first data on the survival, dispersal and mating competitiveness sterile males, all crucial components for determining adequate male release ratios.…”

Section: Sterile Mosquito Releasesmentioning

confidence: 99%

The need for new vector control approaches targeting outdoor biting anopheline malaria vector communities

2020

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Since the implementation of Roll Back Malaria, the widespread use of insecticide-treated nets (ITNs) and indoor residual spraying (IRS) is thought to have played a major part in the decrease in mortality and morbidity achieved in malaria-endemic regions. In the past decade, resistance to major classes of insecticides recommended for public health has spread across many malaria vector populations. Increasingly, malaria vectors are also showing changes in vector behaviour in response to current indoor chemical vector control interventions. Changes in the time of biting and proportion of indoor biting of major vectors, as well as changes in the species composition of mosquito communities threaten the progress made to control malaria transmission. Outdoor biting mosquito populations contribute to malaria transmission in many parts of sub-Saharan Africa and pose new challenges as they cannot be reliably monitored or controlled using conventional tools. Here, we review existing and novel approaches that may be used to target outdoor communities of malaria vectors. We conclude that scalable tools designed specifically for the control and monitoring of outdoor biting and resting malaria vectors with increasingly complex and dynamic responses to intensifying malaria control interventions are urgently needed. These are crucial for integrated vector management programmes designed to challenge current and future vector populations.

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Section: Sterile Mosquito Releasesmentioning

confidence: 99%

The need for new vector control approaches targeting outdoor biting anopheline malaria vector communities

2020

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“…However, this strain has been backcrossed into different An. arabiensis genetic backgrounds [69,70]. In 2018, Lebon and colleagues developed a similar strain in Ae.…”

Section: Conditional Female Death In Late Developmental Stagesmentioning

confidence: 99%

“…Abbreviations: L1, 1st instar larvae; L4, 4th instar larvae; WT, wild type; TR, transgenic; CM, obtained by classical mutagenesis; CT, chemically treated; NC, noncommunicated; Glossina, Glossina p. gambiensis and Glossina pallidipes. See also[15,19,26,[28][29][30]50,51,[53][54][55]59,60,62,64,69,70,72].…”

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confidence: 99%

Sex Sorting for Pest Control: It’s Raining Men!

Lutrat

Giesbrecht

Marois

et al. 2019

Trends in Parasitology

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In the pursuit of better pest-and vector-control strategies, attention returns to an old proven technology, the sterile insect technique (SIT) and related insect population-suppression methods. A major obstacle for any of these approaches that involves the release of sterile males is the separation of males from females during the mass rearing stage, in order to improve the cost-efficiency of these methods and to prevent the release of biting and disease-vectoring females. This review describes recent sex-sorting developments in dipteran flies with an emphasis on assessing the suitability of these methods for large-scale rearing of male vectors for mass release. Sexing Is an Obstacle in Genetic Pest-control ProgramsHighlights Sexing Diptera represents a major obstacle to operationalizing vector-control methods based on the mass release of males, such as the sterile insect technique or the incompatible insect technique.

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“…Before the SIT can be applied at an operational level, there is one critical aspect remaining. This is the development of a robust sex separation system to exclusively obtain males prior to releases [36, 37].…”

Section: Introductionmentioning

confidence: 99%

“…GSS based on insecticide resistance markers have already been developed in An. arabiensis while a tsl -based GSS is currently under development [36, 83, 85].…”

Section: Introductionmentioning

confidence: 99%

A review on the progress of sex-separation techniques for sterile insect technique applications against Anopheles arabiensis

et al. 2018

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The feasibility of the sterile insect technique (SIT) as a malaria vector control strategy against Anopheles arabiensis has been under investigation over the past decade. One of the critical steps required for the application of this technique to mosquito control is the availability of an efficient and effective sex-separation system. Sex-separation systems eliminate female mosquitoes from the production line prior to irradiation and field release of sterile males. This is necessary because female mosquitoes can transmit pathogens such as malaria and, therefore, their release must be prevented. Sex separation also increases the efficiency of an SIT programme. Various sex-separation strategies have been explored including the exploitation of developmental and behavioural differences between male and female mosquitoes, and genetic approaches. Most of these are however species-specific and are not indicated for the major African malaria vectors such as An. arabiensis. As there is currently no reliable sex-separation method for An. arabiensis, various strategies were explored in an attempt to develop a robust system that can be applied on a mass-rearing scale. The progress and challenges faced during the development of a sexing system for future pilot and/or large-scale SIT release programmes against An. arabiensis are reviewed here. Three methods of sex separation were examined. The first is the use of pupal size for gender prediction. The second is the elimination of blood-feeding adult females through the addition of an endectocide to a blood meal source. The third is the establishment of a genetic sexing strain (GSS) carrying an insecticide resistance selectable marker (dieldrin-resistance rdl gene and/or other GABA receptor antagonists that can be used as alternative insecticides to dieldrin) or a temperature-sensitive lethal marker.

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Development of a genetic sexing strain of Anopheles arabiensis for KwaZulu‐Natal, South Africa

Cited by 12 publications

References 24 publications

The need for new vector control approaches targeting outdoor biting anopheline malaria vector communities

The need for new vector control approaches targeting outdoor biting anopheline malaria vector communities

Sex Sorting for Pest Control: It’s Raining Men!

A review on the progress of sex-separation techniques for sterile insect technique applications against Anopheles arabiensis

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