Development of a generic ultra-high-pressure gradient liquid-chromatography method development protocol: The analysis of residual multi-class antibiotics in food products as a case study

Meston, Daniel; Themelis, Thomas; Zhou, Zhuoheng; Vos, Jelle De; Pra, Mauro De; Steiner, Frank; Becue, Ilse; Daeseleire, Els; Desmet, Gert; Eeltink, Sebastiaan

doi:10.1016/j.chroma.2022.463565

Cited by 2 publications

(2 citation statements)

References 21 publications

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“…In addition, we also investigated the parameter of peak symmetry a peak capacity in gradient elution. As listed in Table 1, the tailing factor (T) of six ind compounds were between 0.848 and 1.045, which meets the peak symmetry requiremen This 250 mm chromatographic column can reach the peak capacity of 43 under the op mized conditions [24]. The selection of the HPLC conditions was guided with the requirement for obtaining chromatograms with satisfactory resolutions of the analytes.…”

Section: Quantitative Analysis Of Index Components In Gr By Hplcmentioning

confidence: 98%

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pH as a Key Factor for the Quality Assurance of the Preparation of Gastrodiae Rhizoma Formula Granules

Xie

Min

et al. 2022

Molecules

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Gastrodiae rhizoma (GR) formula granules and preparations have been used as a popular traditional Chinese medicine for clinical treatment since they have good pharmacological activity to treat nervous system diseases. Gastrodin and parishins have been the main active components in aqueous extracts for GR formula granules, but their pharmacological activities and metabolism are different. For quality control of the extracts, the extraction conditions should be investigated to accurately control the contents of two kinds of components. In this paper, the transfer rate of six index components (including gastrodin, p-hydroxybenzyl alcohol, parishin A, parishin B, parishin C, and parishin E) obtained by HPLC were used as indicators to investigate the effect of pH on the GR extraction process. The results demonstrated that pH is a key factor for preventing transforming parishins into gastrodin and maintaining high content of parishins in the extracts. It can be concluded that the weak acid environment could improve the transfer rate of parishins, thus ensuring the gastrodin and parishins consistency between GR raw materials and its aqueous extracts. Therefore, pH is an essential condition for accurate quality control of the extracts.

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Section: Quantitative Analysis Of Index Components In Gr By Hplcmentioning

confidence: 98%

“…As listed in Table 1, the tailing factor (T) of six index compounds were between 0.848 and 1.045, which meets the peak symmetry requirements. This 250 mm chromatographic column can reach the peak capacity of 43 under the optimized conditions [24].…”

Section: Quantitative Analysis Of Index Components In Gr By Hplcmentioning

confidence: 99%

pH as a Key Factor for the Quality Assurance of the Preparation of Gastrodiae Rhizoma Formula Granules

Xie

Min

et al. 2022

Molecules

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Application of carbon quantum dots as fluorescent probes in the detection of antibiotics and heavy metals

Wang,

Zhang,

et al. 2025

Food Chemistry

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Development of a generic ultra-high-pressure gradient liquid-chromatography method development protocol: The analysis of residual multi-class antibiotics in food products as a case study

Cited by 2 publications

References 21 publications

pH as a Key Factor for the Quality Assurance of the Preparation of Gastrodiae Rhizoma Formula Granules

pH as a Key Factor for the Quality Assurance of the Preparation of Gastrodiae Rhizoma Formula Granules

Application of carbon quantum dots as fluorescent probes in the detection of antibiotics and heavy metals

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