Development of a functional thyroid model based on an organoid culture system

Saito, Yoshiyuki; Onishi, Nobuyuki; Takami, Hiroshi; Seishima, Ryo; Inoue, Hirokazu; Hirata, Yuki; Kameyama, Kaori; Tsuchihashi, Kazufumi; Sugihara, Eiji; Uchino, Shigehiko; Ito, Koichi; Kawakubo, Hirofumi; Takeuchi, Hiroya; Kitagawa, Yuko; Saya, Hideyuki; Nagano, Osamu

doi:10.1016/j.bbrc.2018.02.154

Cited by 53 publications

(54 citation statements)

References 30 publications

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“…Organoids of other cancer types have also been faithfully established, such as CC [ 26 ], thyroid cancer [ 80 ], ovarian cancer (OC) [ 81 ], and brain cancer [ 82 ]. CC organoids derived from human metastatic CC biopsies retained rearrangements of fibroblast growth factor receptor 2 that parent tumors harbored [ 26 ].…”

Section: Establishment Of Cancer Organoidsmentioning

confidence: 99%

“…CC organoids derived from human metastatic CC biopsies retained rearrangements of fibroblast growth factor receptor 2 that parent tumors harbored [ 26 ]. Mouse models of poorly differentiated thyroid tumors has been established through the transplantation of the thyroid organoids with enhanced expression of oncogene neuroblastoma RAS derived from mouse with P53 knockout [ 80 ]. In addition, OC cell lines from patients were planted on Matrigel in cancer SC medium containing Gentamicin, Fungizone, and Y27632, and formed organoids with the expression of tumor marker carbohydrate antigen 125 [ 81 ].…”

Section: Establishment Of Cancer Organoidsmentioning

confidence: 99%

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Organoid technology and applications in cancer research

et al. 2018

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During the past decade, the three-dimensional organoid technology has sprung up and become more and more popular among researchers. Organoids are the miniatures of in vivo tissues and organs, and faithfully recapitulate the architectures and distinctive functions of a specific organ.These amazing three-dimensional constructs represent a promising, near-physiological model for human cancers, and tremendously support diverse potential applications in cancer research. Up to now, highly efficient establishment of organoids can be achieved from both normal and malignant tissues of patients. Using this bioengineered platform, the links of infection-cancer progression and mutation-carcinogenesis are feasible to be modeled. Another potential application is that organoid technology facilitates drug testing and guides personalized therapy. Although organoids still fail to model immune system accurately, co-cultures of organoids and lymphocytes have been reported in several studies, bringing hope for further application of this technology in immunotherapy. In addition, the potential value in regeneration medicine might be another paramount branch of organoid technology, which might refine current transplantation therapy through the replacement of irreversibly progressively diseased organs with isogenic healthy organoids.In conclusion, organoids represent an excellent preclinical model for human tumors, promoting the translation from basic cancer research to clinical practice. In this review, we outline organoid technology and summarize its applications in cancer research.

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Section: Establishment Of Cancer Organoidsmentioning

confidence: 99%

Section: Establishment Of Cancer Organoidsmentioning

confidence: 99%

Organoid technology and applications in cancer research

et al. 2018

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“…Tonsil organoids have been used to assess antigen-specific B- and T-cell responses [ 59 ]. The thymus is a primary immune organ, essential for the development of T-lymphocytes, and has been modeled with the use of organoids as well [ 18 , 60 , 61 ]. Moreover, the function of the thymus was recapitulated with the use of synthetic scaffolds and decellularized ECM, in combination with thymic epithelial cells [ 18 ].…”

Section: State-of-the-art Immune-system-on-a-chip Modelsmentioning

confidence: 99%

Immune Organs and Immune Cells on a Chip: An Overview of Biomedical Applications

et al. 2020

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Understanding the immune system is of great importance for the development of drugs and the design of medical implants. Traditionally, two-dimensional static cultures have been used to investigate the immune system in vitro, while animal models have been used to study the immune system’s function and behavior in vivo. However, these conventional models do not fully emulate the complexity of the human immune system or the human in vivo microenvironment. Consequently, many promising preclinical findings have not been reproduced in human clinical trials. Organ-on-a-chip platforms can provide a solution to bridge this gap by offering human micro-(patho)physiological systems in which the immune system can be studied. This review provides an overview of the existing immune-organs-on-a-chip platforms, with a special emphasis on interorgan communication. In addition, future challenges to develop a comprehensive immune system-on-chip model are discussed.

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“…Organoids have been applied to understand stem cell biology, organogenesis and pathogenesis of various diseases (10,18,(20)(21)(22)(23)(24)(25)(26). Organoids have huge potential for modeling cancer and many organoid systems such as breast (27), colorectal (28), and prostate (29) have already been established in experimental studies.…”

Section: Organoidsmentioning

confidence: 99%

The Changing Face of in vitro Culture Models for Thyroid Cancer Research: A Systematic Literature Review

et al. 2020

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Background: Thyroid cancer is the most common endocrine malignancy worldwide. Primary treatment with surgery and radioactive iodine is usually successful, however, there remains a small proportion of thyroid cancers that are resistant to these treatments, and often represent aggressive forms of the disease. Since the 1950s, in vitro thyroid culture systems have been used in thyroid cancer research. In vitro culture models have evolved from 2-dimensional thyrocyte monolayers into physiologically functional 3-dimensional organoids. Recently, research groups have utilized in vitro thyroid cancer models to identify numerous genetic and epigenetic factors that are involved with tumorigenesis as well as test the efficacy of cytotoxic drugs on thyroid cancer cells and identify cancer stem cells within thyroid tumors. Objective of Review: The objective of this literature review is to summarize how thyroid in vitro culture models have evolved and highlight how in vitro models have been fundamental to thyroid cancer research. Type of Review: Systematic literature review. Search Strategy: The National Institute for Health and Care Excellence (NICE) Healthcare and Databases Advanced Search (HDAS) tool was used to search EMBASE, Medline and PubMed databases. The following terms were included in the search: "in vitro" AND "thyroid cancer". The search period was confined from January 2008 until June 2019. A manual search of the references of review articles and other key articles was also performed using Google Scholar. Evaluation Method: All experimental studies and review articles that explicitly mentioned the use of in vitro models for thyroid cancer research in the title and/or abstract were considered. Full-text versions of all selected articles were evaluated. Experimental studies were reviewed and grouped according to topic: genetics/epigenetics, drug testing/cancer treatment, and side populations (SP)/tumor microenvironment (TME). Results: Three thousand three hundred and seventy three articles were identified through database and manual searches. One thousand two hundred and sixteen articles remained after duplicates were removed. Five hundred and eighty nine articles were excluded based on title and/or abstract. Of the remaining 627 full-text articles: Chew et al. Thyroid Cancer in vitro Models 24 were review articles, 332 related to genetic/epigenetics, 240 related to drug testing/treatments, and 31 related to SP/TME. Conclusion: In vitro cell culture models have been fundamental in thyroid cancer research. There have been many advances in culture techniques-developing complex cellular architecture that more closely resemble tumors in vivo. Genetic and epigenetic factors that have been identified using in vitro culture models can be used as targets for novel drug therapies. In the future, in vitro systems will facilitate personalized medicine, offering bespoke treatments to patients.

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Development of a functional thyroid model based on an organoid culture system

Cited by 53 publications

References 30 publications

Organoid technology and applications in cancer research

Organoid technology and applications in cancer research

Immune Organs and Immune Cells on a Chip: An Overview of Biomedical Applications

The Changing Face of in vitro Culture Models for Thyroid Cancer Research: A Systematic Literature Review

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