Development of a fully automated on-line solid phase extraction and high-performance liquid chromatography with diode array detection method for the pharmacokinetic evaluation of bavachinin: A study on absolute bioavailability and dose proportionality

LC‐MS/MS determination of pogostone in rat plasma and its application in pharmacokinetic studies

“…The results indicate that pogostone is absorbed rapidly in rats after oral administration, similar to the pharmacokinetic characteristics in female mice (Li et al ., ). The relatively rapid oral absorption could be due to its low‐polarity characteristic and small molecule size (Liu et al ., ). The elimination half‐life for oral administration of pogostone to rats was 3.76 ± 0.61, 4.26 ± 0.93 and 5.19 ± 1.78 h, respectively.…”

Glucuronidation of bavachinin by human tissues and expressed UGT enzymes: Identification of UGT1A1 and UGT1A8 as the major contributing enzymes

“…Although many studies have shown that BCI possesses a wide range of biological activities, its metabolic pathways and PK properties have not been fully characterized [6]. To the best of our knowledge, only one literature reported the PK properties of BCI in mice via oral and intravenous administration [7]. The PK behaviors of BCI in mice have been characterized as rapid oral absorption, high clearance, and poor absolute bioavailability, while a polar metabolite can be easily detected but its structure has not been identified [7].…”

“…To the best of our knowledge, only one literature reported the PK properties of BCI in mice via oral and intravenous administration [7]. The PK behaviors of BCI in mice have been characterized as rapid oral absorption, high clearance, and poor absolute bioavailability, while a polar metabolite can be easily detected but its structure has not been identified [7]. Our preliminary study on BCI metabolism also found that a monoglucuronide can be rapidly detected following intraperitoneal administration of BCI to mice, implying that BCI could be glucuronidated extensively in mice.…”

“…To date, only one literature has been reported regarding the pharmacokinetics (PK) properties of BCI in mice, in which BCI can be rapidly absorbed but it can be extensively converted to a more polar metabolite [7]. Unfortunately, neither the metabolic pathway nor the major metabolite(s) of BCI in both mice and humans have been identified to date.…”

Characterization of plasma protein binding dissociation with online SPE-HPLC

“…Solid phase extraction (SPE) 13 14 is a sample preparation process developed to enrich and isolate the analytes of interest from matrix. Recently, the fully automated online SPE coupled with high performance liquid chromatography (HPLC) has been established for the quantitative determination of drug concentration in plasma for pharmacokinetic study 15 .…”