Development of a fluorimetric detection method for cinnabarinic acid using <i>ortho</i>‐tolyl hydrazine as the derivatization reagent

Iizuka, Hideaki; Obata, Kensaku; Yajima, Takeaki; Ichiba, Hideaki; Fukushima, Takeshi

doi:10.1002/bmc.1281

Cited by 4 publications

(2 citation statements)

References 18 publications

(13 reference statements)

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“…Pretreatment of rat plasma sample was carried out according to previous paper with minor modification [18,19]. Briefly, rat plasma (10 L) was added to 10 L of 200-M 8-aminocaprylic acid (internal standard, I.S.)…”

Section: Pretreatment Procedures Of Rat Plasmamentioning

confidence: 99%

“…On the basis of the results of the aforementioned studies of d-Trp metabolism, this study attempted to clarify the in vivo pharmacokinetic parameters of d-Trp itself following its administration as compared to that of its enantiomer, l-Trp. Recently, we reported an HPLC method for enantiomeric separation of d,l-Trp and d,l-KYN [18] by pre-column derivatization with [19].…”

Section: Introductionmentioning

confidence: 99%

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Fluorescence determination of d- and l-tryptophan concentrations in rat plasma following administration of tryptophan enantiomers using HPLC with pre-column derivatization

Iizuka

Ishii

Hirasa

et al. 2011

Journal of Chromatography B

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Section: Pretreatment Procedures Of Rat Plasmamentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Fluorescence determination of d- and l-tryptophan concentrations in rat plasma following administration of tryptophan enantiomers using HPLC with pre-column derivatization

Iizuka

Ishii

Hirasa

et al. 2011

Journal of Chromatography B

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Analytical Derivatization Techniques

Parkinson

2012

Comprehensive Sampling and Sample Preparation

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Analytical Derivatization Techniques ☆

Parkinson¹

2014

Reference Module in Chemistry, Molecular Sciences and Chemical Engineering

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For the last 40 years or so, analytical chemists have borrowed well-known reaction techniques from organic and organometallic fields to perform derivatization reactions so as to enhance detection of the elusive analytes they seek. The derivatization mantra has been that, for the technique to be worthwhile, it should be simple to perform, fast (usually less than 15 min), selective, and that the derivatizing reagent should react quantitatively with the analyte and only give one final product. So where are we now? Some derivatization reactions are indeed fast and some are not; some are very selective and some are more universal. However, with a certain care and careful control of optimized conditions, quantitative enhancement outcomes and increased separative power and detection can be very worthwhile. The number of derivatization reagents commercially available itself suggests that there must be some merit to derivatization, that overcomes the associated extra costs: in time (to incorporate the extra reaction step), and in the expense of acquiring, handling, and storing derivatizing reagents. This chapter attempts to show that with proper consideration, it has been feasible to push a chosen analytical technique to give increased selective detection and signal enhancement by derivatization. In many cases, this has made possible the detection of trace components from difficult matrices in a number of scenarios, including on-site analysis, in quality control/continuous analysis methods, or through some remote-sensing platforms. Further, the incorporation of derivatization in an extraction method can be simple and friendly to automated throughput for multiple sample analysis.The fundamental idea of derivatization is that the basic chemical or physical structure of the analytical moiety is changed through a suitable reaction. For example, by labeling compounds for a specific detection purpose, or by changing a functional group to enhance certain chromatographic characteristics, better detection and separation (respectively) can be achieved via the ensuing instrumental techniques. The type of derivatization is highly dependent upon the analytical method and upon the nature of the compound analyzed. There are a variety of good review articles, technical reports, 1 text chapters, 2 and full texts 3-6 that address in detail many specific applications used in analytical derivatization. There are several extraction methods available that are compatible with derivatization. For many analyses, the choice of the best extraction technique is not clear, as many techniques can extract analytes equally well, based on simplicity, applicability, solvent use, expense, detection limit, precision, and time taken to perform the operation. 7 Derivatization techniques have been incorporated into many sample preparation and extractions schemes, to include, but not limited to, total sample headspace, single-drop microextraction (SDME), 8 solid-phase microextraction (SPME), needle trap device (NTD), purge and trap, solid-phase extraction (SPE)...

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Development of a fluorimetric detection method for cinnabarinic acid using ortho‐tolyl hydrazine as the derivatization reagent

Cited by 4 publications

References 18 publications

Fluorescence determination of d- and l-tryptophan concentrations in rat plasma following administration of tryptophan enantiomers using HPLC with pre-column derivatization

Fluorescence determination of d- and l-tryptophan concentrations in rat plasma following administration of tryptophan enantiomers using HPLC with pre-column derivatization

Analytical Derivatization Techniques

Analytical Derivatization Techniques ☆

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