2013
DOI: 10.3724/sp.j.1118.2012.00329
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Development of a fluorescent quantitative polymerase chain reaction technique for detection of grass carp reovirus HZ08 strain

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Cited by 4 publications
(5 citation statements)
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“…Vaccination and early diagnosis is the efficient way for preventing and controlling GCHD (Brudeseth et al, 2013). Numerous techniques have been documented for identifying GCRV infection, such as electron microscopy, virus isolation, nucleic acid sequence amplification plus ELISA (NASA-ELISA), LAMP, and RT-PCR (Liu et al, 2012;Zeng et al, 2013;Wang et al, 2020). Nonetheless, the above technologies are timeconsuming, labor-intensive, nonsensitive, impossible for quantification and need AGE of amplified product.…”
Section: Discussionmentioning
confidence: 99%
“…Vaccination and early diagnosis is the efficient way for preventing and controlling GCHD (Brudeseth et al, 2013). Numerous techniques have been documented for identifying GCRV infection, such as electron microscopy, virus isolation, nucleic acid sequence amplification plus ELISA (NASA-ELISA), LAMP, and RT-PCR (Liu et al, 2012;Zeng et al, 2013;Wang et al, 2020). Nonetheless, the above technologies are timeconsuming, labor-intensive, nonsensitive, impossible for quantification and need AGE of amplified product.…”
Section: Discussionmentioning
confidence: 99%
“…Thus, the GCRV HZ08 in fishes can be identified during the early stages of infection and the disease can be effectively controlled before the infection becomes severe. In a previous study, a real-time PCR was developed with a detection limit of six copies of viral genomic RNA (Liu et al ., 2012). Although the RT-LAMP assay is slightly less sensitive than real-time PCR, it is still considered to be superior as it is a comparatively simple and rapid method which can be performed both in the field and in the laboratory.…”
Section: Discussionmentioning
confidence: 99%
“…In order to test the sensitivity, RNA standards of known concentrations were prepared by fluorescent quantitative PCR technique as previously described (Liu et al ., 2012). A 10 fold series of dilutions spanning 1-10 5 copies per tube was used as template to test the sensitivity of RT-LAMP and RT-PCR for detection of GCRV HZ08.…”
Section: S E N S I T I V I T Y O F Rt -L a M P A N D Rt -P C Rmentioning
confidence: 99%
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“…Grass carp were kindly provided by a fish farm located in Guangzhou (Guangdong, China) with a mean total length and body weight of 20.0 ± 0.5 cm and 100.0 ± 0.5 g, respectively (data are presented as mean values ± SD). Possible viral contamination of the fish but also of the used food was checked by reverse transcription quantitative real‐time PCR (RT‐qPCR) to verify that they were free from different pathogens (Liu et al., ). Animals welfare procedures were performed in compliance with the guidelines of the Animal Experiment Committee, South China Agricultural University.…”
Section: Methodsmentioning
confidence: 99%