Development of a fluorescent label tool based on lanthanide nanophosphors for viral biomedical application

Abstract

We report for the first time the preparation of luminescent lanthanide nanomaterial (LLN) linked bioconjugates and their application as a label tool for recognizing virus in the processing line of vaccine industrial fabrication. Several LLNs with the nanostructure forms of particles or rods/wires with europium (III) and terbium (III) ions in lattices of vanadate, phosphate and metal organic complex were prepared to develop novel fluorescent conjugates able to be applied as labels in fluorescence immunoassay analysis of virus/vaccine.With regard to the LLNs, we have successfully synthesized nanoparticles around 10 nm of YVO 4 : Eu(III), with high emission in the red spectral region, nanorod and nanowire of TbPO 4 • H 2 O and Eu 1−x Tb x PO 4 • H 2 O, width 5-7 nm and length 300 nm, showing very bright luminescence in green, and core/shell nanosized Eu(III) and Tb(III)/Eu(III) complexes with naphthoyl trifluoroacetone and tri-n-octylphosphineoxide (Eu.NTA.TOPO@PVP, Eu X Tb 1−X .NTA.TOPO). The appropriated core/shell structures can play a double role, one for enhancing luminescence efficiency and another for providing nanophosphors with better stability in water media for facilitating the penetration of nanophosphor core into a biomedical environment.The organic functionalizations of the obtained LLNs were done through their surface encapsulation with a functional polysiloxane including active groups such as amine (NH 2 ), thiocyanate (SCN) or mecarpto (SH). The properties of functional sol-gel matrix have great influence on the luminescence properties, especially luminescence intensity of YVO 4 : Eu(III), Eu.NTA.TOPO@PVP, TbPO 4 • H 2 O and Eu x Tb 1−x PO 4 • H 2 O. Bioconjugation processes of the functionalized LLNs have been studied with some bioactive molecules such as biotin, protein immunoglobulin G (IgG) or bovine serum albumin (BSA).The results of LLN-bioconjugate linking with IgG for recognizing virus (vaccine) will be presented in brief. It is consistent to state that the LLN bioconjugates prepared from YVO 4 : Eu(III)-nanoparticles, TbPO 4 • H 2 O nanorod or wire and EuNTA.TOPO@PVP nanosized core/shell complex could be used as labels for recognizing virus in diagnosis or in vaccine production by use of the fluorescence immunoassay (FIA) method. The fluorescence images of the incubated specimens consisting of LLN bioconjugate and vaccine fabricate could be obtained well in terms of sharpness, reproductivity and stability.

Cancer Stem Cell Target Labeling and Efficient Growth Inhibition of CD133 and PD-L1 Monoclonal Antibodies Double Conjugated with Luminescent Rare-Earth Tb3+ Nanorods

“…Among the lanthanides, Tb is a typical lanthanide with strong green fluorescence and has potential for biomedical labeling or imaging. This material has also been studied for use as a carrier for drugs such as a measles virus antibody [15] or cobra venom antigens [10]. Due to the advantages of Tb, we chose this material to produce a nanocomplex, TMC, which is Tb 3+ nanorods double conjugated with CD133 and PD-L1 mAb for the purpose of CSC labeling and therapeutic solution.…”

Advances in Research on Biomedical Nanomaterials in Vietnam

“…Only one year 2012 eleven articles [1][2][3][4][5][6][7][8][9][10][11] have been published. In reference Ha P T et al [1] presented the results of their research on paclitaxel-loaded poly(lactide)-d-α-tocopheryl polyethylene glycol 1000 succinate (PLA-TPGS) nanoparticles prepared by a modified solvent extraction/evaporation technique.…”

“…The output signal of the immunosensors was measured with the change of conductivity resulting from the specific binding of JEV antigens and serum antibodies immobilized on the electrodes with the help of horseradish peroxidax (HRP)-labeled secondary anti-body against JEV. In reference Le QM et al [7] presented the results of the preparation of the luminescent lanthanide nanophosphore (LLN) and bioconjugates linked with LLN as well as their application as label tool for recognizing virus in the processing line of vaccine industrial production. Several LLNs with the nanostructure forms of particles or rods/wires with europium (III) and terbium (III) ions in lattices of vanadate, phosphate and metal organic complex were prepared to develop novel fluorescent conjugates able to be applied as labels in fluorescence immunoassay analysis of virus/ vaccine.…”

Continuous Liquid Phase Synthesis of Europium and Bismuth Co-Doped Yttrium Vanadate Nanophosphor Using Microwave Heating

“…Nanophosphors including quantum dots (QDs) have been extensively investigated during the last two decades due to their potential for display [1]- [5], photovoltaic cells [6]- [8], bio-labels [9], [10], security [11] and so on. Especially, QDs-utilized light emitting diode (LED) backlights realize a wide color gamut display due to their excitonic narrow emission bands [4].…”

“…Nanophosphors are also normally produced via liquid-phase synthses [4], [6], [7], [9], [11], [23]. To obtain an efficient nanophosphor, a long time solvothermal synthesis using an autoclave, which leads a batch reaction with low yield and wide distribution of size and physical properties, is commonly used.…”