Development of a fluorescence aptasensor for rapid and sensitive detection of Listeria monocytogenes in food

Li, Rui; Yun-lian, Zhang; Ali, Shujat; Haruna, Suleiman A.; He, Peihuan; Li, Huanhuan; Ouyang, Qin; Chen, Quansheng

doi:10.1016/j.foodcont.2020.107808

Cited by 63 publications

(19 citation statements)

References 49 publications

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“…The use of LAMP assay for detection in various food products has been reported ( Sun et al, 2015 ; Spielmann et al, 2019 ; Liu et al, 2021 ). However, applying LAMP to detect P. fluorescens in raw milk is rare.…”

Section: Discussionmentioning

confidence: 99%

Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Pseudomonas fluorescens in Raw Milk

Qiao

Zhai

et al. 2022

Front. Microbiol.

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Raw milk is susceptible to microbial contamination during transportation and storage. Pseudomonas fluorescens producing heat-resistant enzymes have become the most common and harmful psychrophilic microorganisms in the cold chain logistics of raw milk. To rapidly detect P. fluorescens in raw milk, the protease gene aprX was selected as a detection target to construct a set of primers with strong specificity, and a loop-mediated isothermal amplification (LAMP) assay was established. The detection thresholds of the LAMP assay for pure cultured P. fluorescens and pasteurized milk were 2.57 × 102 and 3 × 102 CFU/mL, respectively. It had the advantages over conventional method of low detection threshold, strong specificity, rapid detection, and simple operation. This LAMP assay can be used for online monitoring and on-site detection of P. fluorescens in raw milk to guarantee the quality and safety of dairy products.

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Section: Discussionmentioning

confidence: 99%

Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Pseudomonas fluorescens in Raw Milk

Qiao

Zhai

et al. 2022

Front. Microbiol.

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“…It also leads to maternal and neonatal infections, mainly manifesting as sepsis and neuropathy [ 20 ]. In recent years, electrochemiluminescence (ECL) [ 21 ], cell culture [ 22 ], ELISA, and oligonucleotide-based sensors [ 23 ] have been successfully established for the detection of Listeria monocytogenes in the food industry. Nonetheless, the detection sensitivity of the first three methods is not sufficient to determine trace levels of monocytobacter, thus limiting their utility.…”

Section: Biological Contaminationmentioning

confidence: 99%

Review of Electrochemical Biosensors for Food Safety Detection

et al. 2022

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Food safety issues are directly related to people’s quality of life, so there is a need to develop efficient and reliable food contaminants’ detection devices to ensure the safety and quality of food. Electrochemical biosensors have the significant advantages of miniaturization, low cost, high sensitivity, high selectivity, rapid detection, and low detection limits using small amounts of samples, which are expected to enable on-site analysis of food products. In this paper, the latest electrochemical biosensors for the detection of biological contaminants, chemical contaminants, and genetically modified crops are reviewed based on the analytes of interest, electrode materials and modification methods, electrochemical methods, and detection limits. This review shows that electrochemical biosensors are poised to provide miniaturized, specific, selective, fast detection, and high-sensitivity sensor platforms for food safety.

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“…6 It is crucial to develop a rapid, labor-saving, economical and visual detection method for L. monocytogenes. 7 A lateral ow immunoassay (LFIA) strip, as a rapid, userfriendly point-of-care test (POCT) technique, is suitable for the analytical detection of hormones, pathogens and drugs. 8,9 Meantime, various nanomaterials are used as signal labels for LFIA strips, such as colloidal gold, quantum dots and magnetic nanoparticles.…”

Section: Introductionmentioning

confidence: 99%

“…6 It is crucial to develop a rapid, labor-saving, economical and visual detection method for L. monocytogenes . 7…”

Section: Introductionmentioning

confidence: 99%

A novel lateral flow immunoassay strip based on a label-free magnetic Fe₃O₄@UiO-66-NH₂ nanocomposite for rapid detection of Listeria monocytogenes

Liu

et al. 2022

Anal. Methods

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Development of a fluorescence aptasensor for rapid and sensitive detection of Listeria monocytogenes in food

Cited by 63 publications

References 49 publications

Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Pseudomonas fluorescens in Raw Milk

Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Pseudomonas fluorescens in Raw Milk

Review of Electrochemical Biosensors for Food Safety Detection

A novel lateral flow immunoassay strip based on a label-free magnetic Fe₃O₄@UiO-66-NH₂ nanocomposite for rapid detection of Listeria monocytogenes

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Development of a fluorescence aptasensor for rapid and sensitive detection of Listeria monocytogenes in food

Cited by 63 publications

References 49 publications

Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Pseudomonas fluorescens in Raw Milk

Establishment and Evaluation of a Loop-Mediated Isothermal Amplification Assay for Rapid Detection of Pseudomonas fluorescens in Raw Milk

Review of Electrochemical Biosensors for Food Safety Detection

A novel lateral flow immunoassay strip based on a label-free magnetic Fe3O4@UiO-66-NH2 nanocomposite for rapid detection of Listeria monocytogenes

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A novel lateral flow immunoassay strip based on a label-free magnetic Fe₃O₄@UiO-66-NH₂ nanocomposite for rapid detection of Listeria monocytogenes