Development of a EUCAST disk diffusion method for the susceptibility testing of rapidly growing anaerobic bacteria using Fastidious Anaerobe Agar (FAA): a development study using Bacteroides species

Bavelaar, Herjan; Justesen, Ulrik Stenz; Morris, Trefor; Anderson, Bethan; Copsey-Mawer, Sarah; Stubhaug, Tore Taksdal; Kahlmeter, Gunnar; Matuschek, Erika

doi:10.1016/j.cmi.2021.03.028

Cited by 15 publications

(5 citation statements)

References 21 publications

(11 reference statements)

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“…EUCAST are currently developing a method for the disk diffusion tests of anaerobes, 15,16 which would be cheaper and better standardised with a precise methodology and quality control.…”

Section: Discussionmentioning

confidence: 99%

Trends in the prevalence and antibiotic susceptibility of anaerobic Gram-negative bacteria causing clinical infections in Estonia

Lõivukene

Kermes

Sepp

et al. 2023

Sri Lankan J Infec Dis

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Objective:The aim of our study was to describe the species distribution and changes in the resistance profile of anaerobic bacteria isolated at Tartu University Hospital. Methods:The data for 2010, 2016 and 2020 were analysed retrospectively. The strains were identified by Vitek2 (2010) and MALDI-TOF MS (2016MS ( , 2020; and the MIC values for the antibiotics were determined using gradient tests. Resistance was interpreted using EUCAST breakpoints. Results:The average number of anaerobic cultures received during the 10 years increased from 1551 to 5983; (386%).. The most common pathogens were Gram negative anaerobic rodshaped bacteria (Bacteroides fragilis, Bacteroides spp., Fusobacterium spp. and Prevotella spp.). The percentage of susceptible strains in the three years studied was stable for the majority of drug-bug combinations tested, except beta-lactam antibiotic sensitivity (Bacteroides spp. sensitivity to imipenem and ampicillin-sulbactam, Fusobacterium spp. and Prevotella spp. to penicillin).There were significant differences in the MIC values (p < 0.05) when comparing 2020 with 2016. The MIC was higher among Bacterioides spp. ampicillin-sulbactam, Bacteriodes fragilis for imipenem and Prevotella for imipenem, ampicillin-sulbactam and clindamycin in 2020 than in 2016. The same was observed in 2020 vs 2010 among Prevotella spp. MIC for metronidazole, penicillin and cefoxitin. In contrast, the MIC values were surprisingly lower in 2020 than in 2010 for other Gram-negative rods.Conclusions: There was no shift in the spectrum of microbial groups as causative agents of clinical infections during the 2010-2020 period. However, due to the improvement of identification methods, the number of identified species increased. The resistance pattern of anaerobes was stable, but the changes in MIC values may indicate a further steady increase in 2 SLJID • www. http://sljol.info/index.php/SLJID • Vol. 13, No. 1, April 2023 resistance. The surveillance of antibiotic resistance of anaerobes is important to predict the efficiency of empirical treatment.

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“…EUCAST are currently developing a method for the disk diffusion tests of anaerobes, 15,16 which would be cheaper and better standardised with a precise methodology and quality control.…”

Section: Discussionmentioning

confidence: 99%

Trends in the prevalence and antibiotic susceptibility of anaerobic Gram-negative bacteria causing clinical infections in Estonia

Lõivukene

Kermes

Sepp

et al. 2023

Sri Lankan J Infec Dis

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“…Specific structural features, such as functional groups, aromatic systems, stereochemistry, and size/shape, influence the compound's ability to interact with microbial targets and inhibit microbial growth. Understanding this relationship is crucial for identifying key structural determinants and designing effective antimicrobial agents [ [16] , [17] , [18] , [19] ].…”

Section: Methodsmentioning

confidence: 99%

“…The biological activity of the extracts was evaluated as antimicrobial agents against Salmonella typhimurium (S. typhimurium) ATCC 14028 (Gram-negative bacterium), MRSA, ATCC 43300 (Gram-positive bacterium resistant to antibiotics) and Candida albicans (C. albicans) ATCC 60193 (pathogenic yeast) using disk diffusion and two-fold broth microdilution assays according to Bavelaar et al (2021) [ 16 ], Caso Coelho et al (2021) [ 17 ] and Yücesoy et al (2001) [ 18 ]. Briefly, for the disk diffusion method, sterile disks (6 mm) were loaded with 10 mg of the extract and the disk gently was placed on the surface of Mueller–Hinton agar (Scharlab, S.L., Spain) inculcated by tested microorganisms.…”

Section: Methodsmentioning

confidence: 99%

Ephedra alte extracts' GC-MS profiles and antimicrobial activity against multidrug-resistant pathogens (MRSA)

Ayyal Salman,

Yaakop,

Al-Rimawi

et al. 2024

Heliyon

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“…The antibacterial activity of AgNPs prepared from the carex meyeriana Kunth powder was measured using a disk diffusion method (Bavelaar et al, 2021) and the inhibition zone revealed an antibacterial effect. The antibacterial detection medium was autoclaved at 121°C for 15 min.…”

Section: Antibacterial Activity Of Agnps and Agnps Loaded Pva Hydrogelmentioning

confidence: 99%

Preparing and Antimicrobial Activity of Hydrogel with Biosynthesized Silver Nanoparticles Using Carex Meyeriana Kunth

Shen¹,

Zhu²,

Zhou³

et al. 2023

American Journal of Biochemistry and Biotechnology

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In this study, a simple and environmentally stable method was developed to synthesize silver Nanoparticles (AgNPs). In addition, carex meyeriana kunth was used as a reducing and stabilizing agent with good bacterial inhibitory effects against Escherichia coli (E. coli) and Bacillus subtilis at very low doses. AgNPs were added to the Polyvinyl Alcohol (PVA) solution as an antibacterial agent and AgNPs-loaded PVA hydrogel (gel) was formed using the freeze-thaw method. The hydrogel containing AgNPs had a powerful antibacterial effect and the PVA/Ag hydrogel was tested as a trauma dressing, which is a promising composite material for development.

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Development of a EUCAST disk diffusion method for the susceptibility testing of rapidly growing anaerobic bacteria using Fastidious Anaerobe Agar (FAA): a development study using Bacteroides species

Abstract: Development of a EUCAST disk diffusion method for the susceptibility testing of rapidly growing anaerobic bacteria using Fastidious Anaerobe Agar (FAA) -Development study using Bacteroides species, Clinical Microbiology and Infection,

Cited by 15 publications

References 21 publications

Trends in the prevalence and antibiotic susceptibility of anaerobic Gram-negative bacteria causing clinical infections in Estonia

Trends in the prevalence and antibiotic susceptibility of anaerobic Gram-negative bacteria causing clinical infections in Estonia

Ephedra alte extracts' GC-MS profiles and antimicrobial activity against multidrug-resistant pathogens (MRSA)

Preparing and Antimicrobial Activity of Hydrogel with Biosynthesized Silver Nanoparticles Using Carex Meyeriana Kunth

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