Development of a Dynamic Stem Cell Culture Platform for Mesenchymal Stem Cell Adhesion and Evaluation

Ferlin, Kimberly M.; Prendergast, Margaret E.; Miller, Makenzie L.; Nguyen, Bao-Ngoc B.; Kaplan, David S.; Fisher, John P.

doi:10.1021/mp500062n

Cited by 15 publications

(6 citation statements)

References 40 publications

(102 reference statements)

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“…Three-dimensional cell culture on scaffolds may also be improved by other fabrication-independent parameters such as polarity of cell culture plates, culture media and time [ 26 ], as well as different cell culture types, including a dynamic model [ 27 ]. This optimization will be the focus of further studies as we attempt to improve cell attachment percentages.…”

Section: Discussionmentioning

confidence: 99%

Design of a Scaffold Parameter Selection System with Additive Manufacturing for a Biomedical Cell Culture

et al. 2018

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Open-source 3D printers mean objects can be quickly and efficiently produced. However, design and fabrication parameters need to be optimized to set up the correct printing procedure; a procedure in which the characteristics of the printing materials selected for use can also influence the process. This work focuses on optimizing the printing process of the open-source 3D extruder machine RepRap, which is used to manufacture poly(ε-caprolactone) (PCL) scaffolds for cell culture applications. PCL is a biocompatible polymer that is free of toxic dye and has been used to fabricate scaffolds, i.e., solid structures suitable for 3D cancer cell cultures. Scaffold cell culture has been described as enhancing cancer stem cell (CSC) populations related to tumor chemoresistance and/or their recurrence after chemotherapy. A RepRap BCN3D+ printer and 3 mm PCL wire were used to fabricate circular scaffolds. Design and fabrication parameters were first determined with SolidWorks and Slic3r software and subsequently optimized following a novel sequential flowchart. In the flowchart described here, the parameters were gradually optimized step by step, by taking several measurable variables of the resulting scaffolds into consideration to guarantee high-quality printing. Three deposition angles (45°, 60° and 90°) were fabricated and tested. MCF-7 breast carcinoma cells and NIH/3T3 murine fibroblasts were used to assess scaffold adequacy for 3D cell cultures. The 60° scaffolds were found to be suitable for the purpose. Therefore, PCL scaffolds fabricated via the flowchart optimization with a RepRap 3D printer could be used for 3D cell cultures and may boost CSCs to study new therapeutic treatments for this malignant population. Moreover, the flowchart defined here could represent a standard procedure for non-engineers (i.e., mainly physicians) when manufacturing new culture systems is required.

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Section: Discussionmentioning

confidence: 99%

Design of a Scaffold Parameter Selection System with Additive Manufacturing for a Biomedical Cell Culture

et al. 2018

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“…Additionally, Fisher and colleagues recently demonstrated that MSC attachment followed by dynamic substrate movement (stretching) provides one more step in the “education” that MSCs may experience in vivo. 108 The growth of MSCs on soft materials may also preserve the regenerative properties of the cells into later passages or allow them to recover from tissue culture-related aging. 109 The biomaterials-MSC field is actively producing new data, especially in relation to 3D tissue mimetics which may have a profound effect on MSCs, and these data are needed for many growing in vivo applications.…”

Section: Msc Responses—outside-in Signaling On Hard Vs Soft Substratesmentioning

confidence: 99%

Mesenchymal stem cell perspective: cell biology to clinical progress

et al. 2019

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The terms MSC and MSCs have become the preferred acronym to describe a cell and a cell population of multipotential stem/progenitor cells commonly referred to as mesenchymal stem cells, multipotential stromal cells, mesenchymal stromal cells, and mesenchymal progenitor cells. The MSCs can differentiate to important lineages under defined conditions in vitro and in limited situations after implantation in vivo. MSCs were isolated and described about 30 years ago and now there are over 55,000 publications on MSCs readily available. Here, we have focused on human MSCs whenever possible. The MSCs have broad anti-inflammatory and immune-modulatory properties. At present, these provide the greatest focus of human MSCs in clinical testing; however, the properties of cultured MSCs in vitro suggest they can have broader applications. The medical utility of MSCs continues to be investigated in over 950 clinical trials. There has been much progress in understanding MSCs over the years, and there is a strong foundation for future scientific research and clinical applications, but also some important questions remain to be answered. Developing further methods to understand and unlock MSC potential through intracellular and intercellular signaling, biomedical engineering, delivery methods and patient selection should all provide substantial advancements in the coming years and greater clinical opportunities. The expansive and growing field of MSC research is teaching us basic human cell biology as well as how to use this type of cell for cellular therapy in a variety of clinical settings, and while much promise is evident, careful new work is still needed.

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“…The oversimplified system in static culture might lead to misleading results, which in most cases contradict results obtained from in vivo animal experiments. This is because cells under fluid flow behave differently than in static culture due to the activation of flow responsive pathways that might influence their cellular adhesion and nanomaterial uptake (Ferlin et al, 2014;Ye et al, 2018). Photothermal therapy, which kills the cancer cells by heat (Jiang et al, 2020), might remove the tumor completely; hence, it is more superior compared to surgical removal.…”

Section: Introductionmentioning

confidence: 99%

Characterization of MXene as a Cancer Photothermal Agent Under Physiological Conditions

Shurbaji

Manaph

Ltaief

et al. 2021

Front. Nanotechnol.

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A growing interest has recently emerged in the use of nanomaterials in medical applications. Nanomaterials, such as MXene, have unique properties due to their 2D ultra-thin structure, which is potentially useful in cancer photothermal therapy. To be most effective, photothermal agents need to be internalized by the cancer cells. In this study, MXene was fabricated using chemical reactions and tested as a photothermal agent on MDA-231 breast cancer cells under static and physiological conditions. Fluid shear stress (∼0.1 Dyn/cm2) was applied using a perfusion system to mimic the physiological tumor microenvironment. The uptake of MXene was analyzed under fluid flow compared to static culture using confocal microscopy, scanning electron microscopy (SEM), energy dispersive spectrometer (EDS), and transmission electron microscopy (TEM). Furthermore, a viability assay was used to assess cell’s survival after exposing the treated cells to photothermal laser at different power densities and durations. We showed that when incubated with cancer cells, 2D MXene nanoparticles were successfully internalized into the cells resulting in increased intracellular temperatures when exposed to NIR laser. Interestingly, dynamic culture alone did not result in a significant increase in uptake suggesting the need for surface modifications for enhanced cellular uptake under shear stress.

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Development of a Dynamic Stem Cell Culture Platform for Mesenchymal Stem Cell Adhesion and Evaluation

Cited by 15 publications

References 40 publications

Design of a Scaffold Parameter Selection System with Additive Manufacturing for a Biomedical Cell Culture

Design of a Scaffold Parameter Selection System with Additive Manufacturing for a Biomedical Cell Culture

Mesenchymal stem cell perspective: cell biology to clinical progress

Characterization of MXene as a Cancer Photothermal Agent Under Physiological Conditions

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