Development of a Dual Reporter System to Simultaneously Visualize Ca2+ Signals and AMPK Activity
Yusuf C. Erdoğan,
Johannes Pilic,
Benjamin Gottschalk
et al.
Abstract:In this study, we introduce a new separation of phases-based activity reporter of kinase (SPARK) for AMP-activated kinase (AMPK), named AMPK-SPARK, which reports the AMPK activation by forming bright fluorescent clusters. Furthermore, we introduce a dual reporter system, named GCaMP-AMPK-SPARK, by incorporating a single-fluorescent protein (FP)-based Ca 2+ biosensor, GCaMP6f, into our initial design, enabling simultaneous monitoring of Ca 2+ levels and AMPK activity. This system offers the essential quality of… Show more
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