Development of a Dual Recombinant Vaccine To Protect Small Ruminants against Peste-des-Petits-Ruminants Virus and Capripoxvirus Infections

Berhe, Gebretsadik; Minet, Cécile; Goff, Christian Le; Barrett, Thomas; Ngangnou, A.; Grillet, Colette; Libeau, Geneviève; Fleming, M.; Black, D. N.; Diallo, Adama

doi:10.1128/jvi.77.2.1571-1577.2003

Cited by 110 publications

(69 citation statements)

References 29 publications

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“…A VACV double recombinant expressing H and F glycoproteins of RPV has been shown to protect goats against PPR [67] though the animals developed virus-neutralising antibodies only against RPV and not against PPRV. Capripox recombinants expressing the H or F protein of RPV or the F protein of PPRV conferred protection against PPR in goats, but without production of PPRV-neutralizing antibodies [115] or PPRV antibodies detectable by ELISA [22]. Results of these studies suggested that cell-mediated immune responses could play a crucial role in the protection.…”

Section: Immunitymentioning

confidence: 68%

“…The H and F protein genes of several morbilliviruses have been expressed in various vector systems and they can be used as effective sub-unit vaccines. Following this approach, the F protein of PPRV was inserted into the genome of an attenuated capripox vaccine virus candidate [22]. The resulting recombinant virus expressed the PPRV F protein on the surface of infected cells, which was recognized by an anti-F MAb.…”

Section: Recombinant Vaccinesmentioning

confidence: 99%

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Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

130

118

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Peste des petits ruminants (PPR) is an acute, highly contagious, world organization for animal health (OIE) notifiable and economically important transboundary viral disease of sheep and goats associated with high morbidity and mortality and caused by PPR virus. PPR is considered as one of the main constraints in augmenting the productivity of small ruminants in developing countries and particularly severely affects poor farmer's economy. The disease is clinically manifested by pyrexia, oculo-nasal discharges, necrotizing and erosive stomatitis, gastroenteritis, diarrhoea and bronchopneumonia. The disease can be diagnosed from its clinical signs, pathological lesions, and specific detection of virus antigen/antibodies/genome in the clinical samples by various serological tests and molecular assays. PPR is the one of the priority animal diseases whose control is considered important for poverty alleviation in enzootic countries. Availability of effective and safe live attenuated cell culture PPR vaccines and diagnostics have boosted the recently launched centrally sponsored control programme in India and also in other countries. This review article primarily focus on the current scenario of PPR diagnosis and its control programme with advancement of research areas that have taken place in the recent years with future perspectives.

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Section: Immunitymentioning

confidence: 68%

Section: Recombinant Vaccinesmentioning

confidence: 99%

Diagnosis and control of peste des petits ruminants: a comprehensive review

Balamurugan¹,

Hemadri²,

Gajendragad³

et al. 2013

VirusDis.

130

118

View full text Add to dashboard Cite

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“…The common immunogenic properties of these viruses have been used for the preparation of live attenuated vaccines that protect all ruminants against CaPV infection (Kitching et al, 1987). Recombinant CaPVs have also been developed for multivalent vaccination purposes (Berhe et al, 2003;Perrin et al, 2007;Romero et al, 1993;Wade-Evans et al, 1996;Wallace et al, 2006). However, although they are antigenically closely related, restriction enzyme pattern analysis, cross-hybridization studies and, more recently, nucleic acid sequencing have shown that nearly all CaPVs can be grouped according to their host origins Cao et al, 1995;Gershon & Black, 1988;Kitching et al, 1989;Tulman et al, 2002).…”

mentioning

confidence: 99%

Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination

Goff

Lamien

Fakhfakh

et al. 2009

Journal of General Virology

140

142

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The genus Capripoxvirus within the family Poxviridae comprises three closely related viruses, namely goat pox, sheep pox and lumpy skin disease viruses. This nomenclature is based on the animal species from which the virus was first isolated, respectively, goat, sheep and cattle. Since capripoxviruses are serologically identical, their specific identification relies exclusively on the use of molecular tools. We describe here the suitability of the G-protein-coupled chemokine receptor (GPCR) gene for use in host-range grouping of capripoxviruses. The analysis of 58 capripoxviruses showed three tight genetic clusters consisting of goat pox, sheep pox and lumpy skin disease viruses. However, a few discrepancies exist with the classical virus-host origin nomenclature: a virus isolated from sheep is grouped in the goat poxvirus clade and vice versa. Intra-group diversity was further observed for the goat pox and lumpy skin disease virus isolates. Despite the presence of nine vaccine strains, no genetic determinants of virulence were identified on the GPCR gene. For sheep poxviruses, the addition or deletion of 21 nucleic acids (7 aa) was consistently observed in the 59 terminal part of the gene. Specific signatures for each cluster were also identified. Prediction of the capripoxvirus GPCR topology, and its comparison with other known mammalian GPCRs and viral homologues, revealed not only a classical GPCR profile in the last three-quarters of the protein but also unique features such as a longer N-terminal end with a proximal hydrophobic a-helix and a shorter serine-rich C-tail.3These authors contributed equally to this work.Two supplementary tables are available with the online version of this paper.Journal of General Virology (2009), 90, 1967-1977 DOI 10.1099 Davies, 1981Davies, , 1982Diallo & Viljoen, 2007). CaPV infections lead to similar clinical signs in sheep and goats, mainly characterized by fever, excess salivation, conjunctivitis and rhinitis with ocular and nasal discharges. These symptoms are followed by eruption of pox lesions throughout the skin. LSD is a subacute to acute cattle disease with appearance of skin nodules. During epizootics of CaPVs in domestic animals, disease in wild ungulates has never been reported (Davies, 1991), although serology and isolated cases suggests that wildlife species have a possible role in virus maintenance.CaPVs are generally considered to be host-specific, leading to outbreaks in one preferred host. This is partially true since some SPPV and GTPV isolates are capable of causing severe diseases in both sheep and goats (Davies, 1976;Kitching et al., 1989). An in-depth epidemiological investigation to specifically identify the viruses involved in acute small ruminant pox diseases cannot be achieved by serological testing alone due to the very close antigenic relationship between CaPVs, with the existence of only one serotype (Kitching et al., , 1989. The common immunogenic properties of these viruses have been used for the preparation of live attenuated vaccines that ...

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“…The use of filtration and sonication to select for homogeneous LSDV recombinants has proven reproducible and successful in the hands of others (Berhe et al, 2003) and at present remains the method of choice in our laboratory. Advances in selection strategies, which promise to make the selection for homogeneous recombinants even more efficient, such as described by Timiryasova et al (2001), are currently under investigation for use with LSDV.…”

Section: Discussionmentioning

confidence: 99%

“…As for other members of the Poxviridae family, attenuated vaccine strains of LSDV are being developed as vectors for recombinant vaccines for use in the veterinary field. The Kenya Sheep-1 strain, KS-1 ( [Kitching et al, 1987] and [Kitching et al, 1989]), has been utilised for the development of recombinant rinderpest, bluetongue and peste-des-petits-ruminants virus vaccines ( [Romero et al, 1993], [Romero et al, 1994a], [Romero et al, 1994b], [Romero et al, 1994c], [WadeopenUP (December 2007) Evans et al., 1996], [Ngichabe et al, 1997] and [Berhe et al, 2003]). The southern African LSDV vaccine strain, SA-Neethling, was developed from a virulent field isolate, and is a highly host-restricted vaccine that offers long-term protection (Weiss, 1968).…”

Section: Introductionmentioning

confidence: 99%

Improved method for the generation and selection of homogeneous lumpy skin disease virus (SA-Neethling) recombinants

Wallace

Weyer

Nel

et al. 2007

Journal of Virological Methods

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Lumpy skin disease virus (LSDV) is being developed as a vector for recombinant vaccines against diseases of veterinary importance. A strategy for generating viral thymidine kinase (TK) gene-disrupted recombinants which are stable and homogeneous using the South African Neethling vaccine strain of LSDV as vector has been developed.To assist with the selection process, the Escherichia coli β-galactosidase (lacZ) visual marker gene was incorporated into the constructs. However, the use of lacZ has certain limitations. An improved strategy was then devised substituting lacZ with the enhanced green fluorescent protein (EGFP) under control of the vaccinia virus (VV) P11K late promoter. The EGFP marker was found to enhance the selection process, and with the inclusion of additional sonication and filtration steps the number of passages required to select recombinants to homogeneity has been reduced. In support of the improved method for generation and selection of recombinants described, three different LSDV openUP recombinants expressing the glycoprotein genes of bovine ephemeral fever virus, RiftValley fever virus and rabies virus were prepared and characterised.

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Development of a Dual Recombinant Vaccine To Protect Small Ruminants against Peste-des-Petits-Ruminants Virus and Capripoxvirus Infections

Abstract: A recombinant capripoxvirus vaccine containing a cDNA of the peste-des-petits-ruminants virus (PPRV) fusion protein gene was constructed. A quick and efficient method was used to select a highly purified recombinant virus clone. A trial showed that a dose of this recombinant as low as 0

Cited by 110 publications

References 29 publications

Diagnosis and control of peste des petits ruminants: a comprehensive review

Diagnosis and control of peste des petits ruminants: a comprehensive review

Capripoxvirus G-protein-coupled chemokine receptor: a host-range gene suitable for virus animal origin discrimination

Improved method for the generation and selection of homogeneous lumpy skin disease virus (SA-Neethling) recombinants

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