Development of a Dual Mode UCNPs-MB Biosensor in Combination with PCR for Sensitive Detection of Salmonella

Abstract

In recent years, the high prevalence of Salmonella has emerged as a serious threat to public safety, prompting attempts to utilize accurate, rapid, and direct methods to ensure food safety. In this study, a multifunctional platform featuring dual-mode detection channels (colorimetric-fluorescence) combined with polymer chain reaction (PCR) was proposed for the sensitive and rapid detection of Salmonella. Additionally, the colorimetric measurements were achieved by color changes induced by methylene blue (MB) insertion into the double-stranded DNA, and the fluorescence measurements were performed by internal filter effect (IFE)-induced fluorescence quenching of upconversion nanoparticles (UCNPs) by MB. The results showed that the IFE and PCR amplification processes improved the sensitivity of the sensor towards Salmonella detection, with a limit of detection (LOD) of 21.8 CFU/mL. Moreover, this colorimetric-fluorescence dual-mode PCR biosensor was applied to determine Salmonella in food samples, such as chicken, egg, and fish, which produced satisfactory results. Overall, the present study results demonstrate the potential for combining PCR amplification with IFE to develop an efficient and reliable dual-mode analysis platform to safeguard food security.

Nanotechnology’s frontier in combatting infectious and inflammatory diseases: prevention and treatment

“… 715 A dual-mode detection method (colorimetric-fluorescence) combining PCR has been designed to achieve rapid and sensitive detection of Salmonella. 716 CRISPR-based detection systems, such as SHERLOCK, DETECTR, and STOPCovid, are becoming important emerging tools for viral detection due to their speed, specificity, and ease of use. 717 It should be noted that the SHERLOCK test kit and DETECTR test kit have received EUA from the U.S. FDA and can be used for the detection of the SARS-CoV-2 virus.…”