Development of a dual-aptamer-based multiplex protein biosensor

Xie, Shengnan; Walton, S. Patrick

doi:10.1016/j.bios.2010.04.034

Cited by 26 publications

(15 citation statements)

References 60 publications

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“…DNA biosensors based on hybridization of specific-sequence target DNA complementary to the probe sequence or single stranded DNA (ssDNA) have been used in clinical diagnostics, forensic sciences, and biomedical research (Ananthanawat et al 2010;Patel et al 2010;Xie and Walton 2010;Arora et al 2011). DNA is becoming widely used in chemical analysis and is the main component of gene chromosomes in cells (Bostrick et al 2007; J.…”

Section: Introductionmentioning

confidence: 99%

An Electrochemical Biosensor for the Determination ofGanoderma boninensePathogen Based on a Novel Modified Gold Nanocomposite Film Electrode

et al. 2014

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A sensitive approach for the determination of Ganoderma boninense DNA is reported based on an electrochemical affinity system using a modified gold sensor. Covalent attachment of probe DNA was achieved by attachment of the amine group to a carboxylic acid group of a 3,3'-dithiodipropionic acid monolayer on a nanocomposite film of gold nanoparticles bound to poly(3,4-ethylenedioxythiophen)-poly(styrenesulfonate) on a gold working electrode. The electrochemical detection of sequence-specific DNA of probe and target DNA hybridization was monitored using a new ruthenium complex [Ru(dppz) 2 (qtpy)Cl 2 ; dppz = dipyrido [3,2-a:2',3'-c] phenazine; qtpy = 2,2',-4,4".4'4"'-quarterpyridyl redox marker. The potential was selected through the study of the electrochemical behavior of trisaminomethane-hydrochloride containing a ethylenediaminetetraacetic acid supporting electrolyte on the bare and modified gold electrode. The effect of the hybridization temperature and time were measured. The sensor demonstrated specific detection for the target over a concentration range of 1.0 × 10 −15 M to 1.0 × 10 −9 M with a detection limit of 1.59 × 10 −17 M. Control experiments verified the specificity of the biosensor in the presence of a single mismatched DNA sequence. This detection technology was shown to be effective in terms of sensitivity and selectivity of hybridization events and is a promising device for early detection of Ganoderma boninense and other pathogenic threat agents.

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Section: Introductionmentioning

confidence: 99%

An Electrochemical Biosensor for the Determination ofGanoderma boninensePathogen Based on a Novel Modified Gold Nanocomposite Film Electrode

et al. 2014

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“…Two technical or biological explanations could explain this finding: (1) human PDGF-BB specific antibodies were used for capturing bovine PDGF-BB. As mentioned above, this GF is very similar between human and bovine [25]; however, similar PDGF-BB ELISA antibodies (from the same manufacturer) have been used for the same objective in other studies in which bovine PDGF-BB concentrations were measured [26, 27]. (2) It is possible that bovine PDGF-BB presented a high plasma concentration and a nonvariable concentration in PRP as a function of the PLT concentration.…”

Section: Discussionmentioning

confidence: 99%

Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma

Gutiérrez

López

Giraldo

et al. 2017

Veterinary Medicine International

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There is a lack of information about the methods used for bovine platelet-rich plasma (PRP)/platelet-rich gel (PRG) procurement, including information on platelet (PLT), white blood cell (WBC) in PRP, and growth factor release from PRG supernatants. The aims of this study were to compare and to correlate the PLT, WBC, transforming growth factor beta-1 (TGF-β1), and platelet-derived growth factor BB (PDGF-BB) concentrations in bovine whole blood, plasma, and four PRP layers and their respective PRG supernatants: A and B (obtained by a single centrifugation tube method at 720g/5 min) and C and D (obtained by a double centrifugation tube method, by using two centrifugation episodes at 720g/5 min). PLT and WBC counts were significantly higher in PRP-C, followed by whole blood, PRP-A, PRP-B, and PRP-D. TGF-β1 concentrations were significantly higher in PRG-B supernatants and its correspondent PRP-B lysate when compared to the other PRG supernatants and plasma. Supernatants from PRG-A, PRG-B, and PRG-D had equivalent TGF-β1 concentrations. PDGF-BB concentrations were not statistically different between the hemoderivatives. Significant Pearson correlations were noted between PLT counts and WBC counts (0.8) and between PLT counts and PLT distribution width (0.6). Further studies should be performed to assess the potential clinical applications of these PRPs.

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“…However, after the development of aptamer pairs are reported, a few sandwich-type biosensor applications using aptamer pairs have been continuously reported [12–15]. In other words, the successful development of aptamer pairs leads to the research on the development of appropriate sandwich-type biosensors for on-site diagnosis, similar to ELISA kits [16].…”

Section: Introductionmentioning

confidence: 99%

Aptamer-based sandwich-type biosensors

Seo

2017

J Biol Eng

111

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Sandwich-type biosensor platforms have drawn lots of attentions due to its superior features, compared to other platforms, in terms of its stable and reproducible responses and easy enhancement in the detection sensitivity. The sandwich-type assays can be developed by utilizing a pair of receptors, which bind to the different sites of the same target. In this mini-review paper, the sandwich-type biosensors using either pairs of aptamers or aptamer-antibody pairs are reviewed in terms of its targets and platforms, the schematic designs, and their analytical performance.

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Development of a dual-aptamer-based multiplex protein biosensor

Cited by 26 publications

References 60 publications

An Electrochemical Biosensor for the Determination ofGanoderma boninensePathogen Based on a Novel Modified Gold Nanocomposite Film Electrode

An Electrochemical Biosensor for the Determination ofGanoderma boninensePathogen Based on a Novel Modified Gold Nanocomposite Film Electrode

Study of a Two-Step Centrifugation Protocol for Concentrating Cells and Growth Factors in Bovine Platelet-Rich Plasma

Aptamer-based sandwich-type biosensors

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