Development of a double‐layer microfluidic chip with flow medium for chemotherapy resistance analysis of lung cancer

Meng, Qiang; He, Zhijun; Zhang, Lichuan; Zhao, Liang; Li, Encheng; Zhang, Qianqian; Zhang, Xiaolin; Yang, Dongxia; Zou, Lijuan; Gao, Zhancheng; Wang, Qi

doi:10.1002/elps.201100086

Cited by 6 publications

(2 citation statements)

References 32 publications

(28 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The standard CTCs purification protocol would leave few WBCs among them and thus they remains to be identified. To accomplish this, rat-tail collagen (0.012 mg/mL, Solarbio, China) was used to coat the chamber, because CTCs, based on antigen-antibody immune affinity, adhered better on the coated surface 50 .…”

Section: Methodsmentioning

confidence: 99%

An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells

Jiang

Zhao

Wang

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

Recently there has been a more focus on the development of an efficient technique for detection of circulating tumor cells (CTCs), due to their significance in prognosis and therapy of metastatic cancer. However, it remains a challenge because of the low count of CTCs in the blood. Herein, a rapid and high-sensitivity approach for CTCs detection using an integrated microfluidic system, consisting of a deterministic lateral displacement (DLD) isolating structure, an automatic purifying device with CD45-labeled immunomagnetic beads and a capturing platform coated with rat-tail collagen was reported. We observed high capture rate of 90%, purity of about 50% and viability of more than 90% at the high throughput of 1 mL/min by capturing green fluorescent protein (GFP)-positive cells from blood. Further capturing of CTCs from metastatic cancers patients revealed a positive capture rate of 83.3%. Furthermore, our device was compared with CellSearch system via parallel analysis of 30 cancer patients, to find no significant difference between the capture efficiency of both methods. However, our device displayed advantage in terms of time, sample volume and cost for analysis. Thus, our integrated device with sterile environment and convenient use will be a promising platform for CTCs detection with potential clinical application.

show abstract

Section: Methodsmentioning

confidence: 99%

An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells

Jiang

Zhao

Wang

et al. 2017

Sci Rep

Self Cite

View full text Add to dashboard Cite

show abstract

“…Microstructured membranes deployed inside microfluidic channels offer unique advantages in terms of entrapment of cells and analysis of cellular contents. [37][38][39][40][41] The goal of this study was to incorporate aptasensors for cell analysis reported by us previously 25,29 into reconfigurable microfluidic devices. As shown in the schematic of Fig.…”

Section: Microfluidic Device Actuationmentioning

confidence: 99%

Reconfigurable microfluidics with integrated aptasensors for monitoring intercellular communication

et al. 2014

View full text Add to dashboard Cite

We report the development of a microsystem integrating anti-TNF-α aptasensors with vacuum-actuatable microfluidic devices that may be used to monitor intercellular communications. Actuatable chambers were used to expose to mitogen a group of ∼600 cells while not stimulating another group of monocytes only 600μm away. Co-localizing groups of cells with miniature 300μm diameter aptamer-modified electrodes enabled monitoring TNF-α release from each group independently. The microsystem allowed to observe the sequence of events that included 1) mitogenic activation of the first group of monocytes to produce TNF-α, 2) diffusion of TNF-α to the location of the second group of cells and 3) activation of the second group of cells resulting in production of TNF-α by these cells. Thus we were able to experimentally verify reciprocal paracrine cross-talk between the two groups of cells secretion the same signalling molecule. Given the prevalence of such cellular communications during injury, cancer or immune response and the dearth of available monitoring techniques, the microsystem described here is envisioned to have significant impact on cell biology.

show abstract

Microfluidic Organ‐on‐a‐Chip Technology for Advancement of Drug Development and Toxicology

Caplin

Granados

James

et al. 2015

Adv Healthcare Materials

178

124

View full text Add to dashboard Cite

In recent years, the exploitation of phenomena surrounding microfluidics has seen an increase in popularity, as researchers have found a way to use their unique properties to create superior design alternatives. One such application is representing the properties and functions of different organs on a microscale chip for the purpose of drug testing or tissue engineering. With the introduction of "organ-on-a-chip" systems, researchers have proposed various methods on various organ-on-a-chip systems to mimic their in vivo counterparts. In this article, a systematic approach is taken to review current technologies pertaining to organ-on-a-chip systems. Design processes with attention to the particular instruments, cells, and materials used are presented.

show abstract

Development of a double‐layer microfluidic chip with flow medium for chemotherapy resistance analysis of lung cancer

Cited by 6 publications

References 32 publications

An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells

An integrated microfluidic device for rapid and high-sensitivity analysis of circulating tumor cells

Reconfigurable microfluidics with integrated aptasensors for monitoring intercellular communication

Microfluidic Organ‐on‐a‐Chip Technology for Advancement of Drug Development and Toxicology

Contact Info

Product

Resources

About