Development of a dot immunobinding assay to detect <i>Phytophthora</i> spp. in naturally dark‐rooted woody plants

Hahn, Renate; Werres, Sabine

doi:10.1111/j.1744-7348.1997.tb07674.x

Cited by 6 publications

(2 citation statements)

References 18 publications

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“…After the introduction of antibodies as research tools, Phytophthora researchers were quick to investigate the potential use of antibodies in the detection and diagnosis of Phytophthora species. A number of studies of P. cinnamomi , like those of other Phytophthora species, raised polyclonal antibodies against mycelial extracts, but they failed to give species‐specific reactions (Ferraris et al ., 2004; Hahn and Werres, 1997; MacDonald and Duniway, 1979; Malajczuk et al ., 1975). However, in the mid‐1980s, two groups raised monoclonal antibodies against Phytophthora components.…”

Section: P Cinnamomi Identification and Diagnosticsmentioning

confidence: 99%

Phytophthora cinnamomi

Hardham

2005

Molecular Plant Pathology

265

221

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SUMMARYPhytophthora cinnamomi Rands was first isolated from cinnamon trees in Sumatra in 1922. The pathogen is believed to have originated near Papua New Guinea but now has a worldwide distribution. P. cinnamomi is heterothallic with A1 and A2 mating types; however, even in areas in which both mating types are present, it appears that genetic diversity arises asexually rather than as a result of sexual recombination. P. cinnamomi can grow saprophytically in the soil for long periods, rapidly capitalizing on the advent of favourable conditions to sporulate and produce vast numbers of asexual, biflagellate zoospores. The motile zoospores are attracted to suitable infection sites, where they attach and invade the plant. Within a few days, hyphae ramify throughout the tissues of susceptible plants, forming sporangia on the plant surface and rapidly amplifying the disease inoculum. Over the last 10 -15 years, molecular analyses have clarified details of the phylogeny of P. cinnamomi and other Oomycetes. Research on P. cinnamomi has given rise to a more comprehensive understanding of the structure and function of the motile zoospores. New methods have been developed for P. cinnamomi identification and diagnosis. Long-term studies of diseased sites, particular those in southern Australia, have produced a better understanding of the epidemiology of P. cinnamomi diseases. Research has also increased our knowledge of the mode of action and efficacy of inhibitors of P. cinnamomi diseases, especially the phosphonates. This review will present an overview of the advances these studies have made in our understanding of P. cinnamomi pathogenicity, epidemiology and control.Taxonomy: Phytophthora cinnamomi Rands; kingdom Chromista; phylum Oomycota; order Peronosporales; family Peronosporaceae; genus Phytophthora .Host range: Likely to infect in excess of 3000 species of plants including over 2500 Australian native species, and crops such as avocado, pineapple, peach, chestnut and macadamia.Disease symptoms: A root pathogen which usually causes rotting of fine and fibrous roots but which can also cause stem cankers. Often causes dieback of young shoots and is thought to do so as a result of interference with transpiration from roots to shoots.

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Section: P Cinnamomi Identification and Diagnosticsmentioning

confidence: 99%

Phytophthora cinnamomi

Hardham

2005

Molecular Plant Pathology

265

221

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“…Several serological tests have been developed for rapid detection of Phytophthora species (Hardham et al ., 1985; Gabor et al ., 1993; Cahill & Hardham, 1994a,b; Hahn & Werres, 1997; Wakeham et al ., 1997). These tests employ either enzyme‐linked immunosorbent assay (ELISA), membrane trapping assay or a dipstick format.…”

Section: Introductionmentioning

confidence: 99%

Rapid detection of Phytophthora cinnamomi using PCR with primers derived from the Lpv putative storage protein genes

2003

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Phytophthora cinnamomi is an ecologically and economically important pathogen. In this study, PCR assays were developed with primer pair LPV2 or LPV3 for rapid detection and identification of this organism. Both primer pairs were selected from putative storage protein genes. The specificity of these primer pairs was evaluated against 49 isolates of P. cinnamomi , 102 isolates from 30 other Phytophthora spp., 17 isolates from nine Pythium spp. and 43 isolates of other water moulds, bacteria and true fungi. PCR with both primer pairs amplified the DNA from all isolates of P. cinnamomi regardless of origin. The LPV3 primers showed adequate specificity among all other species tested. The LPV2 primers cross-reacted with some species of Pythium and true fungi, but not with any other Phytophthora species. PCR with the LPV3 primers detected the pathogen at levels of a single chlamydospore or 10 zoospores in repeated tests. The PCR assay was at least 10 times more sensitive than the plating method for detection of the pathogen from artificially infested soilless medium, and, to a lesser extent, from naturally infected plants. PCR with LPV3 primers can be a useful tool for detecting P. cinnamomi from soilless media and plant tissues at ornamental nurseries, whereas the LPV2 primers can be an effective alternative for identification of this species from pure culture. Applications of these assays for detection of P. cinnamomi in other environments were also discussed.

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