Development of a dose-response bioassay for stanniocalcin in fish

Wagner, Graham F.; Niu, Ping; Jaworski, Ewa; Radman, Dennis P.; Chiarot, Cameron

doi:10.1016/s0303-7207(97)04009-4

Cited by 19 publications

(11 citation statements)

References 26 publications

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“…This mechanism may be similar to the one proposed to initiate the release of the hypocalcemic hormone stanniocalcin (STC), in which the CaR is thought to be involved (34). The release of STC from the corpuscles of Stannius during acute hypercalcemia is a critical mechanism leading to rapid reductions in the rate of branchial Ca 2ϩ uptake (5,19,30,42) thought to involve modification of Ca 2ϩ conductance through existing Ca 2ϩ channels. The results of the present study demonstrate that chronic hypercalcemia may lower Ca 2ϩ uptake by an additional mechanism, a reduction in the number of ECaCs.…”

Section: Low Environmental Camentioning

confidence: 85%

Hormonal and environmental regulation of epithelial calcium channel in gill of rainbow trout (Oncorhynchus mykiss)

Shahsavarani

Perry

2006

American Journal of Physiology-Regulatory, Integrative and Comp

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Section: Low Environmental Camentioning

confidence: 85%

Hormonal and environmental regulation of epithelial calcium channel in gill of rainbow trout (Oncorhynchus mykiss)

Shahsavarani

Perry

2006

American Journal of Physiology-Regulatory, Integrative and Comp

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“…A bioassay originally developed for testing the effects of the hormone STC was used (Wagner et al 1997). This bioassay monitors the effects of the hormone on whole body 45 Ca uptake which is considered to be a reliable indicator of GCAT in fish.…”

Section: The Effects Of Salmon Ct On Gcat In Rainbow Troutmentioning

confidence: 99%

“…This bioassay monitors the effects of the hormone on whole body 45 Ca uptake which is considered to be a reliable indicator of GCAT in fish. For the present studies CT was used instead of STC, but otherwise the format of the bioassay was used as described (Wagner et al 1997).…”

Section: The Effects Of Salmon Ct On Gcat In Rainbow Troutmentioning

confidence: 99%

“…The rate of GCAT in these fish rises significantly as a result of the lower plasma STC levels. Because of these effects, phosphate-loading heightens the sensitivity of trout to exogenous STC in terms of its ability to inhibit GCAT (Wagner et al 1997). As our working hypothesis was that CT would inhibit GCAT, we reasoned that it would be preferable to reduce as much as possible the influence of STC in order to best observe any inhibitory effects of CT on GCAT.…”

Section: The Effects Of Salmon Ct On Gcat In Rainbow Troutmentioning

confidence: 99%

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Salmon calcitonin inhibits whole body Ca2+ uptake in young rainbow trout

Wagner

Jaworski²,

Radman³

1997

Journal of Endocrinology

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Gill Ca2+ transport (GCAT) in fish is regulated by a number of different hormones. Stanniocalcin (STC) from the corpuscles of Stannius (CS) is an inhibitor of GCAT, whereas pituitary-derived prolactin and cortisol stimulate GCAT. Other than this, however, little is known about the effects of other hormones on this important transport process. The role of calcitonin (CT) in calcium homeostasis in fish is still controversial. Whereas many studies have shown significant effects of CT on plasma calcium levels, an equal number of studies have failed to find any correlations between plasma calcium and CT levels in fish. Previous in vitro studies have shown that salmon CT has potent inhibitory effects on GCAT in isolated, perfused fish gill preparations, a finding that has never been corroborated in vivo. Therefore, in this report we examined the effects of salmon CT on whole body 45Ca uptake (as a measure of GCAT) in young rainbow trout. In support of the in vitro findings, we found that CT had significant inhibitory effects on GCAT. In parallel studies, we found that CT had no effects on STC secretion and only modest, stimulatory effects on STC mRNA levels in cultured trout CS cells. These finding suggest that both CT and STC function as negative regulators of GCAT in fish.

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“…Although stanniocalcin secreted from the corpuscles of Stannius is hypocalcemic (or antihypercalcemic factor) (46), there is strong evidence that parathyroid hormone-related protein (PTHrP) has strong short-term hypercalcemic actions on calcium balance (7,21,24). A variety of other endocrine factors described as having long-term calcitropic actions in fish include prolactin (18), vitamin D (42), estrogen (22,34), and cortisol (17).…”

mentioning

confidence: 99%

Regulation of calcium balance in the sturgeonAcipenser naccarii: a role for PTHrP

Fuentes

Haond²,

Guerreiro³

et al. 2007

American Journal of Physiology-Regulatory, Integrative and Comp

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Calcium regulation in sturgeon is of special interest because they are a representative of the ancient fishes possessing mainly cartilaginous skeletons and a supposedly low calcium demand. The present study aimed to characterize the effect of a chronic absence of dietary calcium and the effect of parathyroid hormone-related protein (PTHrPA) (1-34) (7) on calcium balance in juvenile sturgeon (Acipenser naccarii). At rest, sturgeon juveniles are in net positive calcium balance, since whole body calcium uptake is significantly higher than efflux and calcium accumulates in the body. To study the importance of dietary calcium, the sturgeon were kept on a calcium-free diet for 8 wk. This manipulation impaired growth as measured by failure to gain weight or increase in length and indicates that dietary calcium is important for growth in sturgeon. An increased whole body calcium uptake partially compensated dietary calcium deficiency and was associated with increased gill chloride cell number in lamellae and filaments in parallel with increased gill Na(+)K(+)-ATPase activity. In addition, a single injection of piscine PTHrP(1-34) significantly increased whole body calcium uptake and decreased whole body calcium efflux. Administration of PTHrP significantly increased circulating plasma calcium 4-24 h postinjection. The increase in net calcium transport and increased plasma levels of calcium is consistent with the actions of a hypercalcemic factor. It would appear that the sturgeon rely on calcium for growth and tightly regulate calcium transport. The action in calcium balance is consistent with PTHrP acting as a hypercalcemic factor in sturgeon.

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Development of a dose-response bioassay for stanniocalcin in fish

Cited by 19 publications

References 26 publications

Hormonal and environmental regulation of epithelial calcium channel in gill of rainbow trout (Oncorhynchus mykiss)

Hormonal and environmental regulation of epithelial calcium channel in gill of rainbow trout (Oncorhynchus mykiss)

Salmon calcitonin inhibits whole body Ca2+ uptake in young rainbow trout

Regulation of calcium balance in the sturgeonAcipenser naccarii: a role for PTHrP

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