Development of a DNA sensor using a molecular logic gate

Bhattacharjee, D.; Dey, Dibyendu; Chakraborty, Santanu; Hussain, Syed Arshad; Sinha, Sangram

doi:10.1007/s10867-012-9295-3

Cited by 18 publications

(11 citation statements)

References 39 publications

(80 reference statements)

Supporting

Mentioning

Contrasting

Order By: Relevance

“…This is an indication that the presence of DNA enhances the FRET. It is interesting to mention in this context that in one of our previous work we have investigated the influence of DNA in the FRET between Acf and RhB in solution phase [24]. Based on that investigation we have demonstrated a DNA sensor [24].…”

Section: Fret Between Acf and Rhb In Presence And Absence Of Dnamentioning

confidence: 94%

Sensing of DNA conformation based on change in FRET efficiency between laser dyes

Dey

Saha

Roy

et al. 2014

Sensors and Actuators B: Chemical

Self Cite

View full text Add to dashboard Cite

This communication reports the effect of DNA conformation on fluorescence resonance energy transfer (FRET) efficiency between two laser dyes in layer by layer (LbL) self assembled film. The dyes Acraflavine and Rhodamine B were attached onto the negative phosphate backbones of DNA in LbL film through electrostatic attraction. Then FRET between these dyes was investigated. Increase in pH or temperature causes the denaturation of DNA followed by coil formation of single stranded DNA. As a result the FRET efficiency also changed along with it. These observations demonstrated that by observing the change in FRET efficiency between two laser dyes in presence of DNA it is possible to detect the altered DNA conformation in the changed environment.

show abstract

Section: Fret Between Acf and Rhb In Presence And Absence Of Dnamentioning

confidence: 94%

Sensing of DNA conformation based on change in FRET efficiency between laser dyes

Dey

Saha

Roy

et al. 2014

Sensors and Actuators B: Chemical

Self Cite

View full text Add to dashboard Cite

show abstract

“…Over the last two decades a numerous sophisticated fluorescence based spectroscopic assays have been developed in order to study biological process and to study the behaviour of biomolecules both in vitro (freely diffusing or immobilized molecules) and in living cells . Among these assays, FRET between two fluorophores has gained much interest due to its potential to probe the different biomolecular processes [2][3][4][5][6][7]. FRET has been widely used in all applications of fluorescence including medical diagnostics, DNA analysis, optical imaging and for various sensing properties [2][3][4][5][6][7].…”

Section: Introductionmentioning

confidence: 99%

“…Among these assays, FRET between two fluorophores has gained much interest due to its potential to probe the different biomolecular processes [2][3][4][5][6][7]. FRET has been widely used in all applications of fluorescence including medical diagnostics, DNA analysis, optical imaging and for various sensing properties [2][3][4][5][6][7]. Generally, fluorescence-based sensors adopt three different strategies: (a) fluorescence quenching (turn-off), (b) fluorescence enhancement (turn-on) and (c) ratiometric FRET.…”

Section: Introductionmentioning

confidence: 99%

Fluorescence resonance energy transfer (FRET) as biomarkers

Paul

Suklabaidya

Hussain

2021

Materials Today: Proceedings

View full text Add to dashboard Cite

“…DNA‐based logic gates have been reported to perform Boolean logic operations, such as XOR or AND, where the resulting output is either 0 or 1 depending on the applied inputs . NOT, AND, and XOR logical operations were also accomplished by DNAzymes in which the presence or absence of a Förster/fluorescence resonance energy transfer (FRET) signal was used as output .…”

Section: Introductionmentioning

confidence: 99%

Construction of a Fuzzy and Boolean Logic Gates Based on DNA

Zadegan

Jepsen

Hildebrandt

et al. 2015

Small

View full text Add to dashboard Cite

Logic gates are devices that can perform logical operations by transforming a set of inputs into a predictable single detectable output. The hybridization properties, structure, and function of nucleic acids can be used to make DNA-based logic gates. These devices are important modules in molecular computing and biosensing. The ideal logic gate system should provide a wide selection of logical operations, and be integrable in multiple copies into more complex structures. Here we show the successful construction of a small DNA-based logic gate complex that produces fluorescent outputs corresponding to the operation of the six Boolean logic gates AND, NAND, OR, NOR, XOR, and XNOR. The logic gate complex is shown to work also when implemented in a three-dimensional DNA origami box structure, where it controlled the position of the lid in a closed or open position. Implementation of multiple microRNA sensitive DNA locks on one DNA origami box structure enabled fuzzy logical operation that allows biosensing of complex molecular signals. Integrating logic gates with DNA origami systems opens a vast avenue to applications in the fields of nanomedicine for diagnostics and therapeutics.

show abstract

Development of a DNA sensor using a molecular logic gate

Cited by 18 publications

References 39 publications

Sensing of DNA conformation based on change in FRET efficiency between laser dyes

Sensing of DNA conformation based on change in FRET efficiency between laser dyes

Fluorescence resonance energy transfer (FRET) as biomarkers

Construction of a Fuzzy and Boolean Logic Gates Based on DNA

Contact Info

Product

Resources

About