Development of a direct quantitative detection method for Meloidogyne incognita and M. hapla in andosol and analysis of relationship between the initial population of Meloidogyne spp. and yield of eggplant in an andosol

Abstract

Baermann method, while that of M. incognita and M. hapla from 0.6 to 713 J2 equivalent (J2eq)/20 g soil and from 0.0 to 115 J2eq/20 g soil, respectively, with the real-time PCR method. The yield was determined by the sum of commercial sized eggplants harvested for 3 months of the cultivation period. The yield decreased in the plots with an initial population of RKNs more than 2 J2/20 g soil with the Baermann method. In real-time PCR, the yields were low in the plots with the sum of initial M. incognita and M. hapla more than 128 J2eq/20 g soil. The present study established a quantification method with real-time PCR for M. incognita and M. hapla in andosol and evaluated the relationship between the initial population of Meloidogyne spp. and the yield of eggplant. Nematol. Res. 43(2), 21-29 (2013)

Differences in parasitism of Meloidogyne incognita and two genotypes of M. arenaria on Solanum torvum in Japan

“…in the tropics (Sasser, 1979). Recently, Watanabe et al (2013) reported that the yield of eggplant was decreased by an initial population of Meloidogyne spp. consisting of more than two-second-stage juveniles per 20 g soil.…”

Reproduction of <i>Meloidogyne incognita </i>on eggplant rootstock cultivars and effect of eggplant rootstock cultivation on nematode population density

“…(M. incognita is dominant) in the tropics (Sasser, 1979) and the economic threshold level of eggplant is reported to be 5.4 M. incognita second-stage juveniles (J2) per 100 g soil (Netcher and Sikora, 1990). Recently, Watanabe et al (2013) reported that soil densities of two J2 per 20 g soil or greater with the Baermann technique have been shown to significantly reduce eggplant yield (cultivar Senryo 2go) at 50 days after planting.…”

“…(M. incognita is dominant) in the tropics (Sasser, 1979) and the economic threshold level of eggplant is reported to be 5.4 M. incognita second-stage juveniles (J2) per 100 g soil (Netcher and Sikora, 1990). Recently, Watanabe et al (2013) reported that soil densities of two J2 per 20 g soil or greater with the Baermann technique have been shown to significantly reduce eggplant yield (cultivar Senryo 2go) at 50 days after planting.Most plant nematode control methods rely on chemical agents. Although soil fumigants such as 1,3-dichloropropene are commonly used, the use of such chemicals is likely to be subject to increasing restriction owing to environmental and safety concerns.…”

A real-time PCR based assay as an alternative to a seedling assay to evaluate potential risk of <i>Meloidogyne incognita </i>

“…Density of plant parasitic nematodes is measured with real-time PCR and relations between densities and damage to crops have been evaluated e.g. in Pratylenchus penetrans (Cobb) Filipjev and Schuurmans Stekhoven against radish , M. incognita against eggplant (Watanabe et al, 2013) and Hirschmanniella diversa Sher against lotus (Koyama et al, 2013). However, the relationship between the density of M. incognita and damage to tomato is not yet established.…”

“…Watanabe et al (2013) reported that eggplant' s yield losses were observed in plots with an initial population of M. incognita and M. hapla Chitwood more than 128 J2eq/20 g soil measured with real-time PCR.…”