Development of a direct and visual isothermal method for meat adulteration detection in low resource settings

Yan, Chao; Wang, Xiudan; Zhao, Xiaoli; Wei, Manman; Shi, Chao; Ma, Cuiping

doi:10.1016/j.foodchem.2020.126542

Cited by 8 publications

(5 citation statements)

References 39 publications

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“…This method could detect the presence of 0.1% foreign meat and showed no cross-reactivity with 14 other animal species. Yan et al developed a test to identify duck meat admixture [50]. The authors used the SEA method as the base of the test system.…”

Section: Meat Identification Methods That Do Not Use Chromatographic ...mentioning

confidence: 99%

“…On the other Figure 2. The example of a scheme for identifying the presence of foreign admixtures in a semi-finished meat product using SEA [50] Figure 3. The example of a proteome obtained with the help of gel electrophoresis: 1 -raw pork, 2 -heat-treated pork [14] hand, the emergence of new types of sorbents for thin layer chromatography will also contribute to a better separation of the components being under study, which sorbents will improve the performance of the developed test systems.…”

Section: Prospects For Further Developmentmentioning

confidence: 99%

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Review of new technologies used for meat identification

Kornienko

Fomina²,

Minaev

2022

Teor. prakt. pererab. mâsa (Print)

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The present article represents an analysis of trends in development of test-systems for identification of meat. These test systems are commonly used in food production and research laboratories. The relevance of development of methods for identification of meat kinds is related not only to the food restrictions, which are practiced in some religions and related to consumption of certain types of meat, but also with the hygienic aspects of food production. Also, this research is inspired also by the acute issue of food products adulteration and the replacement of one type of meat with another one. The article considers the trends in the develop ment of microanalysis method that use immunochromatic research, i. e. methods based on molecular biology. Also this article considers the devices that do not use chromatographic methods of analysis. Examples of the development of test systems based on various methods of analysis for the identification of meat are given below. Attention is focused on the prospects of combining these methods, including colorimetric methods for identification of meat. It is also specified that the emergence of new dyes and new enzyme systems, suitable for use in enzyme-immunoassay, can enhance the sensitivity of these test systems. It is also noted that the development of technologies associated with sorbents can contribute to a better separation of the test substrates and this way to increase the sensitivity of the test in case of small amounts of test substrate. It is also noted that the use of various types of iso thermal amplification can reduce the analysis time necessary for meat identification. Various schemes of devices for microanalysis are given; their advantages and disadvantages are listed. An example of proteomes application for meat identification is given. It is shown that this method can also be applied in the heat treatment of meat. The prospects for the development of such devices are analyzed. It is concluded that the development of systems for microanalysis in the form of quick tests is quite relevant and promis ing. It is indicated that theoretically in the future such analytical systems, due to the use of microfluidic technologies, will be able to combine several methods. The authors proposed to use machine-aided cognition methods to analyze data obtained from similar test systems in order to increase their sensitivity.

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Section: Meat Identification Methods That Do Not Use Chromatographic ...mentioning

confidence: 99%

Section: Prospects For Further Developmentmentioning

confidence: 99%

Review of new technologies used for meat identification

Kornienko

Fomina²,

Minaev

2022

Teor. prakt. pererab. mâsa (Print)

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“…Some antibody‐independent NA‐LFA methods have also been developed in which biotinylated probes or amplicons have been used with SA or poly(dT) sequence as a binding molecule (Kortli et al., 2020; Magiati, Myridaki, Christopoulos, & Kalogianni, 2019; Ngom, Guo, Wang, & Bi, 2010). A number of NA‐LFAs or NA‐LFS or NA‐LFD devices have been developed for POC detection of animal species in different meat products (Figure 1) (Li et al., 2019; Yan et al., 2020; Zhang, Zhang, et al., 2019; Zhao, Wang, et al., 2020; Zheng et al., 2020).…”

Section: Poc Testing Devicesmentioning

confidence: 99%

“…The method had the sensitivity of 0.1% horse meat and showed no cross‐reactivity with 14 other animal species. A visual on‐site detection method was developed for the identification of duck meat (Yan et al., 2020). The method was based on denaturation bubble‐mediated SEA.…”

Section: Recent Developments In Poc Devices For Meat Species Identifimentioning

confidence: 99%

Rapid point‐of‐care testing methods/devices for meat species identification: A review

Kumar

Narsaiah

2020

Comp Rev Food Sci Food Safe

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The authentication of animal species is an important issue due to an increasing trend of adulteration and mislabeling of animal species in processed meat products. Polymerase chain reaction is the most sensitive and specific technique for nucleic acid‐based animal species detection. However, it is a time‐consuming technique that requires costly thermocyclers and sophisticated labs. In recent times, there is a need of on‐site detection by point‐of‐care (POC) testing methods and devices under low‐resource settings. These POC devices must be affordable, sensitive, specific, user‐friendly, rapid and robust, equipment free, and delivered to the end users. POC devices should also confirm the concept of micro total analysis system. This review discusses POC testing methods and devices that have been developed for meat species identification. Recent developments in lateral flow assay‐based devices for the identification of animal species in meat products are also reviewed. Advancements in increasing the efficiency of lateral flow detection are also discussed.

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“…9,10 Different from other NAT methods, this method excels at the amplification of short fragments and has been widely used in the detection of foodborne pathogens. [11][12][13] For infectious pathogens with high variation rates, it is tough to find a long, unique and conserved sequence for primer or probe designing. The advantage of the SEA technique in amplifying short targets will shed light on the detection of infectious pathogens with high variation rates.…”

Section: Introductionmentioning

confidence: 99%