Development of a Detection Method against 11 Major Pathogens of Honey Bee using Amplification of Multiplex PCR and Specific DNA-chip

Rapid detection of deformed wing virus in honeybee using ultra-rapid qPCR and a DNA-chip

“…Generally, DNA-chip-based methods are suitable for a high degree of multiplexing, can be used to detect multiple pathogens, can simultaneously subtype the target agents, and can be easily adapted to accommodate viral variants [17]. Thus far, 11 major pathogens of honeybee have been detected by DNA-chip-based methods, using specific probes for each pathogen [21]. In this study, we used a DNA-chip on which DWV-specific probes had been spotted onto the chip surface and confirmed that Suwon and Yeosu honeybee samples contained DWV RNA molecules.…”

Application of ultra‐rapid qPCR and DNA chips for viral RNA detection and confirmation

“…The cross‐section of the DNA‐chip in the form of a glass rod was capable of fine‐aligning a total of 49 oligonucleotides (7 × 7), and a total of four HC probes were micro‐arrayed to identify positions. The glass rod of the DNA chip was immersed in the PCR amplification solution in the 200 μL centrifuge tube, and the signal was confirmed by the hybridization of the probe and the PCR product on the cross‐section of the glass rod . The probes used for the DNA‐chip are shown in Table .…”