Development of a defined medium fermentation process for physostigmine production by Streptomyces griseofuscus

Zhang, J.; Marcin, C.; Shifflet, M. A.; Salmon, Peter; Brix, T.; Greasham, Randolph; Buckland, Barry C.; Chartrain, Michel

doi:10.1007/s002530050601

Cited by 9 publications

(9 citation statements)

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“…Thus, bioprocess intensification focuses on optimizing fermentation yields via media composition and feed strategies, dynamic control of physical conditions, induction, genetics, immobilization, and bioreactor engineering. Much of the work in antibiotic production has been carried out on nonmarine microbes, mainly fungi (Nagamune et al, 1988;Vanek et al, 1990;Zhang et al, 1996), which is in contrast to the small body of work published on marine microbes. This review highlights areas in which empirical and mechanistic knowledge of terrestrial antibiotic fermentations (both fungal and bacterial) could be applied to marine bacteria.…”

Section: Bioprocess Intensificationmentioning

confidence: 70%

“…Phosphate limitation was instrumental in stimulating phenazine-1-carboxylic acid synthesis by P. aurofaciens (Turner and Messenger, 1986) and phystigimine production by Streptomyces griseofuscus (Zhang et al, 1996). Phenazine production by P. phenazinium was found not to be regulated by phosphate level (Messenger and Turner, 1983), highlighting the fact that regulation of secondary metabolism can be species specific.…”

Section: Phosphatementioning

confidence: 71%

“…Actinomycete isopenicillin synthase was found to be sensitive to ammonium levels (Demain, 1986). However, Zhang et al (1996) found ammonium to stimulate an antibiotic produced by Streptomyces griseofuocus. Control of ammonia concentration during the mid-cycle was found to be important in the optimization of idiophase secondary metabolite production (Junker et al, 1998), though this may reflect the role of nitrogen in growth promotion.…”

Section: Nitrogenmentioning

confidence: 99%

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Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

1999

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: There is a lack of research into bioreactor engineering and fermentation protocol design in the field of marine bacterial antibiotic production. Most production strategies are carried out at the shake-flask level and lack a mechanistic understanding of the antibiotic production process, offering poor prospects for successful scale-up. This review shows that data need to be collated on media and physical optima differences between the trophophase and idiophase, along with investigations into the control mechanisms for biosynthesis, to allow implementation of novel fermentation protocols. Immobilization may play a part in bioprocess intensification of marine bacterial antibiotic production, through again this area is understudied. Similarly, mass transfer and shear stress data of fermentations are needed to provide the bioreactor design requirements to intensify antibiotic biosynthesis, with process scale-up in mind. The application of bioprocess intensification methods to the production of antibiotics (and other metabolites) from marine microbes will become an important strategy for improving supply of natural products, in order to assess their suitability as chemotherapeutic drugs.

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Section: Bioprocess Intensificationmentioning

confidence: 70%

Section: Phosphatementioning

confidence: 71%

Section: Nitrogenmentioning

confidence: 99%

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Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

1999

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“…In many studies, complex media (e.g., TB medium) are used as standard medium. However, for bioprocesses, mineral media are favored over complex media as they are generally cheaper, comprise more defined reaction conditions, and simplify downstream processing [77]. Moreover, auxotrophy selection markers present on the yeast expression vectors for cyp1a1 genes require the use of mineral media.…”

Section: Resultsmentioning

confidence: 99%

Comparison of microbial hosts and expression systems for mammalian CYP1A1 catalysis

Cornelissen

Julsing

Schmid

et al. 2012

Journal of Industrial Microbiology and Biotechnology

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Mammalian cytochrome P450 enzymes are of special interest as biocatalysts for fine chemical and drug metabolite synthesis. In this study, the potential of different recombinant microorganisms expressing rat and human cyp1a1 genes is evaluated for such applications. The maximum specific activity for 7-ethoxyresorufin O-deethylation and gene expression levels were used as parameters to judge biocatalyst performance. Under comparable conditions, E. coli is shown to be superior over the use of S. cerevisiae and P. putida as hosts for biocatalysis. Of all tested E. coli strains, E. coli DH5α and E. coli JM101 harboring rat CYP1A1 showed the highest activities (0.43 and 0.42 U g⁻¹(CDW), respectively). Detection of active CYP1A1 in cell-free E. coli extracts was found to be difficult and only for E. coli DH5α, expression levels could be determined (41 nmol g⁻¹(CDW)). The presented results show that efficient expression of mammalian cyp1a1 genes in recombinant microorganisms is troublesome and host-dependent and that enhancing expression levels is crucial in order to obtain more efficient biocatalysts. Specific activities currently obtained are not sufficient yet for fine chemical production, but are sufficient for preparative-scale drug metabolite synthesis.

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“…RSM is a powerful and efficient mathematical approach widely applied in the optimization of fermentation processes, e.g. medium components for enzyme production (Adinarayana and Ellaiah 2002;Bernal et al 2006;Anbu et al 2007;Cai and Zheng 2009;Sharma et al 2009), production of other metabolites (Zhang et al 1996) and biomass production (Lohomme and Roux 1991;Rucka et al 1998). The method can give information about the interaction between variables, provide information necessary for design and process optimization, reduce the number of experiments, and give multiple responses at the same time (Box and Behnken 1960;Chang et al 2002;Jain and Nian-fa 2007).…”

Section: Introductionmentioning

confidence: 99%

Optimizing the biodegradation of two keratinous wastes through a Bacillus subtilis recombinant strain using a response surface methodology

2010

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Statistical optimization of the biodegradation of two keratinous wastes directed by Bacillus subtilis recombinant cells was carried out by means of a response surface methodology. A Box-Behnken design was employed to predict the optimal levels of three variables namely, keratin percent, incubation time and inoculum size. Analysis of variance revealed that, only keratin percent had the highest significant effect. Canonical analysis and ridge max analysis were used to get the optimal levels of the three predictors along with the optimum levels of the responses. The optimal sets of predicted and validated levels of the three variables were [7.69% (w/v) feathers, 96.58 h and 1.28% (v/v) inoculum size] and [8% (w/v) feathers, 98.45 h, 3.9% (v/v) inoculum size] to achieve the highest levels of soluble proteins (1.25-1.7 mg/ml) and NH(2)-free amino groups (245.82-270.0 μmol leucine/ml), respectively upon using three optimized feathers-based media. These values represented 83.67-100% and 100% adequacy for the models of soluble proteins and NH(2)-free amino groups, respectively. While, [8.23% (w/v) sheep wool, 5.52% (v/v) inoculum size and 46.58 h] and [8.33% (w/v) sheep wool, 5.89% (v/v) inoculum size and 63.46 h] were the optimal sets of predicted and validated levels of the above variables to achieve the highest yields of soluble proteins (3.4-4.6 mg/ml) and NH(2)-free amino groups (290.9-302.0 μmol leucine/ml), respectively upon using three optimized sheep wool-based media. These values represented 100% adequacy for the models of soluble proteins and NH(2)-free amino groups. By the end of the optimization strategy, a fold enhancement (2.14-2.43 and 1.78-2.12) in the levels of released soluble proteins and NH(2)-free amino groups, respectively was obtained upon using three optimized feathers-based media. However, a fold enhancement (4.25-5.75 and 2.42-2.5) in the levels of soluble proteins and NH(2)-free amino groups, respectively was obtained upon using three optimized sheep wool-based media. Data would encourage pilot scale optimization of the biodegradation of these wastes.

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Development of a defined medium fermentation process for physostigmine production by Streptomyces griseofuscus

Cited by 9 publications

References 0 publications

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

Bioprocess Intensification for Production of Novel Marine Bacterial Antibiotics Through Bioreactor Operation and Design

Comparison of microbial hosts and expression systems for mammalian CYP1A1 catalysis

Optimizing the biodegradation of two keratinous wastes through a Bacillus subtilis recombinant strain using a response surface methodology

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