Development of a CZE Method for the Determination of Olmesartan Medoxomil in Tablets

Çelebi̇er, Mustafa; Altınöz, Sacide

doi:10.1365/s10337-007-0424-2

Cited by 27 publications

(21 citation statements)

References 13 publications

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“…In micellar electrokinetic chromatography (MEKC), largely used in the analysis of neutral pharmaceuticals, a surfactant is added to the running buffer in a concentration higher than its critical micelle concentration, generating a pseudo stationary phase, in which solute partition takes place. 17 Currently, there are published methods using CE for the determination of OLM in coated tablets, 18 its degradation kinetic, 19 and combined with other drugs, 20,21 but no methods are available using MEKC. Thus, the goal of this study was to develop and validate a stability-indicating method using MEKC for the determination of this drug in solid pharmaceutical dosage forms, according to the current International Conference on Harmonization (ICH) guidelines, 22,23 performing a comparison with the previously validated LC method, thereby contributing to the development of new alternatives techniques for the quality control of pharmaceutical formulations.…”

Section: Introductionmentioning

confidence: 99%

Stability-indicating comparative methods using mekc and lc for determination of olmesartan medoxomil

et al. 2013

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Recebido em 24/2/12; aceito em 25/7/12; publicado na web em 11/12/12 A stability-indicating method using MEKC was validated for the analysis of olmesartan medoxomil in tablets. Successful separation was achieved using a fused silica capillary (40 cm x 50 μm i.d.); background electrolyte consisted of a combination of 10 mmol L -1 borate buffer and 5 mmol L -1 anionic detergent sodium dodecyl sulfate (95:5; v/v) pH 6.5; hydrodynamic mode at 50 mBar for 5 s; 25 kV separation voltage at 25 °C; and column temperature 25 °C with detection at 257 nm. The proposed method, validated following ICH guidelines, was applied to the determination of this antihypertensive with good results compared with an LC method.

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Section: Introductionmentioning

confidence: 99%

Stability-indicating comparative methods using mekc and lc for determination of olmesartan medoxomil

et al. 2013

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“…A RP-HPLC assay was reported for the separation of related substances in olmesartan medoxomil 6 . Celebier et al presented an UV-VIS spectrophotometry and Capillary Zone Electrophoresis methods for the determination of olmesartan medoxomil in tablets [7][8] . A validated absorption factor spectrophotometric and HPLC methods for analysis of olmesartan medoxomil in combined pharmaceutical formulations were published 9,10 .…”

Section: Introductionmentioning

confidence: 99%

Acid- Alkali Degradation Study on Olmesartan Medoxomil and Development of Validated Stability-Indicating Chromatographic Methods

Moussa

Mohamed

Youssef

2010

J. Chil. Chem. Soc.

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A reversed phase liquid chromatography (RP-HPLC) and thin layer chromatography (HPTLC) densitometry methods as a stability indicating assays of olmesartan medoxomil in presence of its acid or alkaline induced degradation products were developed. Olmesartan medoxomil and its degradation products were analyzed by HPLC equipped with UV-Variable wave length detector at 257 nm where quantitation was achieved by isocratic elution on Agilent, Exclipse XDB-C 18 column with mobile phase composed of acetonitrile: methanol: water: glacial acetic acid (40:35:25:0.1 v/v/v/v) at flow rate 1ml min.-1 . HPTLC was performed on aluminum packed Nano silica gel 60 F 254 TLC plates as stationary phase with significant difference in Rƒ values between olmesartan medoxomil and its degradates using chloroform: methanol: formic acid (8:1.5:0.5 v/v/v) as mobile phase. Densitometric evaluation of intact drug was carried out at 260 nm. The calibration curve of olmesartan medoxomil in bulk form was linear from 0.5-10 µg ml -1 and 0.05-1 mg ml -1 with mean percentage accuracy 99.97 ± 1.085 % and 100.35 ± 1.060 % for HPLC and HPTLC methods, respectively. The two proposed methods were successfully applied for the determination of olmesartan medoxomil in drug substance and in drug product. Methods validation was tested for linearity; accuracy; precision; selectivity and robustness, according to USP guidelines.

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“…A literature survey revealed that several analytical methods were reported for its determination. These methods include high-performance thin-layer chromatography (3), liquid chromatography (3)(4)(5)(6)(7)(8)(9), and capillary zone electrophoresis (10). Also, a few spectrophotometric methods have been reported for the analysis of OLM in pharmaceutical tablets (11)(12)(13).…”

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confidence: 99%

High throughput microwell spectrophotometric assay for olmesartan medoxomil in tablets based on its charge-transfer reaction with DDQ

Darwish¹,

Wani²,

Khalil³

et al. 2014

Acta Pharmaceutica

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The study describes the development and validation of a new microwell-based spectrophotometric assay for determination of olmesartan medoxomil (OLM) in tablets. The formation of a colored charge-transfer (CT) complex between OLM as an n-electron donor and 2,3-dichloro--5,6-dicyano-1,4-benzoquinone (DDQ) as a p-electron acceptor was investigated, and employed as the basis for the development of the new assay. The proposed assay was conducted in 96-microwell plates. The absorbance of the colored-CT complex was measured at 460 nm with a microplate reader. Optimum conditions of the reaction and the analytical procedures of the assay were established. Under the optimum conditions, a linear relationship with a good correlation coefficient was found between the absorbance and the concentration of OLM in the range of 2-200 µg per well. The limits of detection and quantitation were 0.53 and 1.61 µg per well, respectively. No interference was observed from the excipients present in OLM tablets or from hydrochlorothiazide and amlodipine besylate that were co-formulated with OLM in some of its formulations. The assay was successfully applied to the analysis of OLM in tablets with good accuracy and precision. The assay described herein has a great practical value in the routine analysis of OLM in quality control laboratories, since it has a high throughput property and consumes low volumes of organic solvent. It thus offers a reduction in the exposure of analysts to the toxic effects of organic solvents, as well as a reduction in the cost of analysis.

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Development of a CZE Method for the Determination of Olmesartan Medoxomil in Tablets

Cited by 27 publications

References 13 publications

Stability-indicating comparative methods using mekc and lc for determination of olmesartan medoxomil

Stability-indicating comparative methods using mekc and lc for determination of olmesartan medoxomil

Acid- Alkali Degradation Study on Olmesartan Medoxomil and Development of Validated Stability-Indicating Chromatographic Methods

High throughput microwell spectrophotometric assay for olmesartan medoxomil in tablets based on its charge-transfer reaction with DDQ

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