Development of a cytometric bead array screening tool for the simultaneous detection of pro-inflammatory cytokines in porcine plasma

Multiplex analysis of pro-inflammatory cytokines in serum of Actinobacillus pleuropneumoniae-infected pigs

“…The applicability of the CBA 3-plex assay for serum samples was evaluated prior to use. The limit of detection (LOD) as well as intraand inter-assay coefficients of variation (CVs) were assessed and compared to the values previously obtained in porcine plasma (Wyns et al, 2013).…”

“…Validation parameters for serum samples are summarized in Table 1 and compared to those previously obtained in porcine plasma by our group (Wyns et al, 2013).…”

“…In contrast to humans and rodent species, multiplex assays for porcine research are scarce. During the past decade, several multiplex bead-based assays have been in-house developed to characterize the immune response in pigs (Johannisson et al, 2006;Bjerre et al, 2009;Lawson et al, 2010;Bongoni et al, 2013;Wyns et al, 2013).…”

“…The present study was designed to test out serum samples of A. pleuropneumoniae-infected pigs at the onset of infection for proinflammatory cytokines using a novel developed cytometric bead array (CBA) 3-plex assay for a fast and simultaneous profiling of TNF-α, IL-1β and IL-6, as previously described for porcine plasma in an LPS-induced inflammation model by Wyns et al (2013), with special attention to the IL-6 response.…”

“…With the exception of the bead selection (A4, C7 and D9 for IL-6, IL-1β and TNF-α, respectively), antibodies, beads and standards were applied as previously described (Wyns et al, 2013). Standard curves of 0.0, 0.125, 0.25, 0.5, 1.0, 2.5, 5.0, 7.5, 10.0, 20.0 and 50.0 ng/mL were …”