Development of a cross-priming isothermal amplification assay based on the glycoprotein B gene for instant and rapid detection of feline herpesvirus type 1

Tan, Yurong; Dong, Guoying; Xu, Hefeng; Niu, Jiangting; Lu, Wei; Wang, Kai; Dong, Hao; Zhang, Shuang; Huang, Hailong; Hu, Guang‐Wan

doi:10.1007/s00705-020-04526-5

Cited by 8 publications

(6 citation statements)

References 25 publications

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“…FHV191071 and FHV191072 particles were similar in size and shape to those of herpesviruses isolated from animals (15,18). PCR detection methods (e.g., real-time and priming isothermal amplification assays) have previously been used to detect FHV-1 (4,12). PCR amplification of FHV-1 based on the TK and gB genes yielded bands of the expected size, which confirmed the identity of FHV-1.…”

Section: Discussionmentioning

confidence: 59%

“…The diagnosis of FHV-1 infection is based on virus isolation with cell cultures, pathological findings, the direct fluorescent antibody test, polymerase chain reaction (PCR), real-time PCR, and serological assays (4,12,13). Virus neutralizing antibody titers measured with the virus neutralization assay are used to monitor sero-epidemiological status or to evaluate the immunogenicity of vaccination (14).…”

Section: Introductionmentioning

confidence: 99%

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Isolation and Molecular Characterization of Feline Herpesvirus 1 from Naturally Infected Korean Cats

Yang

Kim

Park

et al. 2020

jbv

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Feline herpesvirus type 1 (FHV-1) causes respiratory and ocular disease in cats. Although isolates of FHV-1 circulating in cats have been reported worldwide, Korean FHV-1 isolates and their features have not been reported thus far. We aimed to investigate the biological and molecular characterization of two FHV-1 isolates based on the nucleotide sequence of thymidine kinase (TK) and glycoprotein B (gB) gene. In total, 48 samples from 12 cats were prepared for virus isolation. For the diagnosis, virus isolation, indirect fluorescence assay (IFA), electron microscopy (EM), and polymerase chain reaction (PCR) and for the molecular characterization, cloning and sequencing were used. Based on many methods such as virus isolation with specific cytopathic effects, IFA, EM, and PCR, two isolates were confirmed as FHV-1 and they showed the highest viral titer (10 8.3 to 10 8.5 TCID 50 /mL) in the Crandell-Rees Feline Kidney cells at 48 h after inoculation, but did not grow in MDCK and Vero cells. The nucleotide and amino acid sequences of the full TK and gB gene of FHV191071 and FHV191072 isolates were determined and compared with those of other herpesvirus strains. Two isolates possessed the same nucleotide sequences belonging to FHV-1 group and had the highest similarity (99.9%) with the KANS-02 strain, which was isolated from shelter in USA in 2016. Two isolates were confirmed as FHV-1 and they will be a useful basic resource for evaluating current FHV-1 vaccine and developing diagnostic tools.

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Section: Discussionmentioning

confidence: 59%

Section: Introductionmentioning

confidence: 99%

Isolation and Molecular Characterization of Feline Herpesvirus 1 from Naturally Infected Korean Cats

Yang

Kim

Park

et al. 2020

jbv

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“…(III) Peptide synthesis and serum samples detection: the serum of TB patients and control serum (non-TB patients and healthy people) were used to evaluate the serological detection value of different peptides to obtain effective antibody-binding peptide and its detection method. For specific methods, refer to our published article (22,23).…”

Section: Screening Of Antibody Binding Peptidesmentioning

confidence: 99%

Accuracy of multitarget indirect enzyme-linked immunoassay assay for detection of tuberculosis antibody

Hu¹,

Liu²,

Hu³

et al. 2021

Ann Transl Med

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Background: Diagnosis of tuberculosis (TB) is still difficult. The development of rapid and sensitive laboratory tools for the diagnosis of tuberculosis is a priority. This study aimed to develop an indirect enzyme-linked immunoassay (ELISA) assay for detection of TB antibody and explore its diagnostic value in patients with pulmonary tuberculosis (PTB) via a multi-center clinical evaluation. Methods:The specific antigen, fusion antigen, and specific antibody peptide were obtained using molecular cloning and phage peptide library screening. An indirect ELISA assay was developed using multiple target materials. Further, the assay was validated in six institutions with clinically confirmed TB patients, non-TB patients with pulmonary disease, and healthy controls as research subjects.Results: An indirect ELISA assay was developed with 16 kD antigen, 11,488 (CFP10-MPT48-TB8.4) fusion antigen, and TB18 and pl2 as target antigens against TB antibody. The results of this multicenter study showed that the sensitivity, specificity, and the area under the receiver operating characteristic curve (AUC) of the assay were 48.25% [95% confidence interval (CI): 45.5-51.1%], 92.20% (95% CI: 90.7-93.5%) and 0.724 (95% CI: 0.707-0.741), respectively, and the cut-off value was 0.119. According to the meta-analysis, the combined ROC was 0.736 (95% CI: 0.692-0.779), I 2 =83.73%. The sensitivity of the sputum-positive PTB group (culture or smear positive) was 58.75% (95% CI: 52.96-65.00%); the sensitivity in sputum-negative group (culture or smear negative) was 37.38% (95% CI: 32.71-42.52%), respectively; the sensitivity of the sputum-positive group was significantly higher than that of sputum-negative group (OR =1.57, 95% CI: 1.29-1.92, P<0.001).Conclusions: Multitarget indirect ELISA assay based on specific-TB antigen, fusion antigen, and antibody peptide is of value for the diagnosis of PTB and can be used as an auxiliary rapid diagnostic tool to improve the sensitivity of sputum-negative TB.

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“…( Ou et al., 2014 ; Wang et al., 2018 ) CPA technology has been used for rapid diagnosis of many infectious diseases, such as babesia bovis infection, scale drop disease virus infetion, avian leukosis virus subgroup J infection, african swine fever virus and feline herpesvirus type 1 (FHV-1) infecton, Bacillus cereus infection, COVID-19 infetion and so on. ( Woźniakowski et al., 2018 ; Tan et al., 2020 ; Zhai et al., 2020 ; Prasitporn et al., 2021 ; Wang et al., 2021 ; Wu et al., 2021 ; Xiang et al., 2021 ) To ensure sensitivity and specificity, it targets the multiple-copy gene IS6110 of MTBC and is performed at a constant temperature 63°C. It has been reported that CPA can be used to diagnose pulmonary tuberculosis and peripheral tuberculosis.…”

Section: Introductionmentioning

confidence: 99%

Research of childhood tuberculosis in suspected populations by molecular methods: A multicenter study in China

Wang²,

Huang³

et al. 2022

Front. Cell. Infect. Microbiol.

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The research of childhood tuberculosis is inadequate in china. The cross-priming amplification (CPA) of specific DNA in clinical samples is increasingly adopted for the diagnosis of childhood tuberculosis. In this study, a multicenter research was performed to investigate the incidence and characteristics of childhood tuberculosis in suspected populations mainly by CPA method. 851 children suspected of tuberculosis were enrolled in seven centers across China. All samples were tested by a CPA method and 159 subjects were tested by Xpert MTB/RIF and liquid culture method in parallel to assess the reliability of the CPA method. A positive result in any one of the three methods provided a definitive diagnosis of Mycobacterium tuberculosis complex (MTBC) infection. The MTBC-positive rate was 9.5% (81/851) by the combined methods; 93.8% of the cases were detected by CPA technology (76/81). The rate of pulmonary infection was significantly higher than that of extrapulmonary infection (7.1%, 60/851 vs 2.5%, 21/851; P < 0.001). Scrofula was the predominant type of extrapulmonary tuberculosis. The MTBC positive rates in 12-18-year-old group (middle school), was 28.4% (23/81), higher than in those under-six-year-old (preschool; 39/525) and the 6~11-year-old (primary school; 18/235) groups combined (P < 0.001). The MTBC positive rate in patients with a clear history of tuberculosis exposure was significantly higher than in cases in which there was no history of tuberculosis contact(35.3%, 18/51 vs 7.8%, 61/782; P < 0.001). In conclusion, this multicenter investigation showed that pulmonary tuberculosis and extrapulmonary tuberculosis are not uncommon in children in China, with teenagers being particularly susceptible to infection. The incidence of pulmonary tuberculosis in children is higher than that of extrapulmonary tuberculosis. History of exposure to tuberculosis is a high risk factor for childhood tuberculosis.

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Development of a cross-priming isothermal amplification assay based on the glycoprotein B gene for instant and rapid detection of feline herpesvirus type 1

Cited by 8 publications

References 25 publications

Isolation and Molecular Characterization of Feline Herpesvirus 1 from Naturally Infected Korean Cats

Isolation and Molecular Characterization of Feline Herpesvirus 1 from Naturally Infected Korean Cats

Accuracy of multitarget indirect enzyme-linked immunoassay assay for detection of tuberculosis antibody

Research of childhood tuberculosis in suspected populations by molecular methods: A multicenter study in China

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