Development of a cost-effective medium for the large scale production of Bacillus thuringiensis var israelensis

Prabakaran, G.; Balaraman, K

doi:10.1016/j.biocontrol.2005.09.018

Cited by 55 publications

(35 citation statements)

References 11 publications

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“…3). Medium 3 had the highest biomass production with high toxicity compared to the conventional and commercial lab medium (Table 2), similarly to what was observed by Prabakaran & Balaraman (2006). All media showed a decrease in cell mass production around 72h of fermentation (Fig.…”

Section: Resultssupporting

confidence: 77%

Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide

Valicente¹,

Mourão²

2008

Neotrop. entomol.

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Section: Resultssupporting

confidence: 77%

Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide

Valicente¹,

Mourão²

2008

Neotrop. entomol.

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“…The salts contained in the mixture have been one of the most frequently found in the culture media optimization for the production of spores [12]- [14]; because they are involved in the gene expression for dipicolinic acid synthesis, a specific component of bacterial endospores which is responsible of spore heat resistance [15]- [17].…”

Section: Sporulation Induction With Individual Factorsmentioning

confidence: 99%

Improvement of Sporulation Conditions of a New Strain of <i>Bacillus amyloliquefaciens</i> in Liquid Fermentation

Díaz-García¹,

García-Riaño²,

Zapata-Narvaez³

2015

ABB

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The desirable active ingredients for the development of bioproducts based in Bacillus sp. for the control of soil pathogens are the spores because these structures exhibit more resistance and stability to conditions present during the fermentation, formulation, and storage processes. To improve the sporulation of a native strain of Bacillus amyloliquefaciens (Bs006) using liquid fermentation, some modifications in the concentrations of the components in a previously standardized culture media were made. Subsequently, five sporulation inducers (iron nitrate, mixture of salts, peroxide hydrogen, temperature, and initial cell concentration) were evaluated. The treatment with a mixture of salts in combination with an initial cell concentration of 1 × 10 8 cells/ml was selected because a final spore concentration of 1.05 × 10 10 spores/ml after 66 hours with a fully substrate consumption and sporulation efficiency of 88.7% was obtained. To demonstrate the biological activity of B. amyloliquefaciens Bs006 in Cape gooseberry seedlings, a greenhouse bioassay was conducted, and statistical differences in plant growth-promoting parameters compared with previous media were not found. Additionally, the proposed modified media (coded as JM) presented a benefit-cost ratio 2.65 times higher compared with the baseline media.

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“…En el Himalaya han encontrado más aplicaciones relacionadas con esta enzima, por cuanto presenta buenos resultados como aditivo a detergentes debido a la compatibilidad que muestra con el cloro (36,37), y a la recuperación de radiografías usadas. En la purificación de dos nuevas proteasas extracelulares fue encontrada la serin proteasa (38) con una actividad proteasa del 98%, por lo que nuevos estudios se han encaminado a la biosíntesis de esta enzima a gran escala, su producción es dependiente de la fuente de nitrógeno del medio, por lo cual se han utilizado extractos de levadura, peptona y glucosa como alternativas nutricionales que han mostrado buenos resultados (39,40).…”

Section: Enzimasunclassified

Bacillus sphaericus: biocontrolador de vectores que producen malaria, fiebre amarilla y dengue

Romero

Rodríguez

Ramírez

2009

nova

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ResumenEl aumento en la incidencia de enfermedades de interés en salud pública como malaria, dengue y fiebre amarilla asociado a los efectos secundarios del uso de plaguicidas químicos y la resistencia generada, ha impulsado a la búsqueda de nuevas alternativas para la erradicación de sus vectores. Una opción viable como bacteria entomopatógena es el Bacillus sphaericus, descrita por primera vez en 1904 por Neide y Meyer, quienes descubrieron una bacteria formadora de esporas, que existe en la naturaleza y que crece fácilmente tanto in vitro como en el cadáver de las larvas.Su actividad larvicida se debe a la producción de una inclusión cristalina denominada toxina binaria A y B de 41,9 y 51,4 kDa, respectivamente, las cuales, al ser liberadas en el intestino de los insectos susceptibles en su fase larvaria y tras ser solubilizada por el pH alcalino en la porción media del intestino, activan las proteasas causando deshidratación y muerte a los vectores. Es considerada como un biolarvicida altamente potencial y eficaz para el control biológico de Anopheles spp., Aedes spp. y Culex spp. Comercialmente puede producirse por fermentación sumergida a pequeña escala con materiales proteínicos de desecho. Este producto ha sido utilizado y registrado en numerosos países de América Latina, donde se han obtenido buenos resultados después de su aplicación.Palabras clave: Bacillus sphaericus, biocontrolador, biolarvicida, toxina binaria Bin A y Bin B. Abstract Bacillus sphaericus: vector control strategies that produce malaria, yellow fever, and dengue The increased incidence of diseases of public health such as malaria, dengue and yellow fever associated to side effects of the use of chemical pesticides and the resistance generated has prompted the search for new alternatives for the elimination of its vectors. A viable option is the entomopathogenic bacteria Bacillus sphaericus, first described in 1904 by Neide and Meyer who discovered spore-forming bacteria that exists in nature and is easily grown in vitro and in the body of the larvae.Its larvicidal activity is due to the production of a crystalline inclusion, called binary toxin A and B of 41.9 and 51.4 kDa, respectively, which, when released in the gut of susceptible insects in their larval ARTÍCULO DE REVISIÓN

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Development of a cost-effective medium for the large scale production of Bacillus thuringiensis var israelensis

Cited by 55 publications

References 11 publications

Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide

Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide

Improvement of Sporulation Conditions of a New Strain of <i>Bacillus amyloliquefaciens</i> in Liquid Fermentation

Bacillus sphaericus: biocontrolador de vectores que producen malaria, fiebre amarilla y dengue

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Development of a cost-effective medium for the large scale production of Bacillus thuringiensis var israelensis

Cited by 55 publications

References 11 publications

Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide

Use of by-products rich in carbon and nitrogen as a nutrient source to produce Bacillus thuringiensis (Berliner)-based biopesticide

Improvement of Sporulation Conditions of a New Strain of &lt;i&gt;Bacillus amyloliquefaciens&lt;/i&gt; in Liquid Fermentation

Bacillus sphaericus: biocontrolador de vectores que producen malaria, fiebre amarilla y dengue

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Improvement of Sporulation Conditions of a New Strain of <i>Bacillus amyloliquefaciens</i> in Liquid Fermentation