“…In raw shrimp shells, protein (26.0 ± 0.53%) and ash (47.4 ± 2.2%) content were preferably high ( Table 1 ), which are consistent with the previous studies ( Ghorbel-Bellaaj et al, 2011 ; Hamdi et al, 2017a , b ). The existence of proteins and ash with main components of minerals make shrimp shell hard, which blocks the release of chitin from shrimp shells.…”
Section: Resultssupporting
confidence: 91%
“…Where M O and M R are ash content (%) before and after fermentation; O and R represent the mass (g) of the original sample and fermented residue on a dry weight basis, respectively ( Hamdi et al, 2017a , b ).…”
As an environmentally friendly and efficient method, successive two-step fermentation has been applied for extracting chitin from shrimp shells. To screen out the microorganisms for fermentation, a protease-producing strain, Exiguobacterium profundum, and a lactic acid-producing strain, Lactobacillus acidophilus, were isolated from the traditional fermented shrimp paste. Chitin was extracted by successive two-step fermentation with these two strains, and 85.9 ± 1.2% of protein and 95 ± 3% of minerals were removed. The recovery and yield of chitin were 47.82 and 16.32%, respectively. Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy (SEM) were used to characterize the chitin. The crystallinity index was 54.37%, and the degree of deacetylation was 3.67%, which was lower than that of chitin extracted by the chemical method. These results indicated that successive two-step fermentation using these two bacterial strains could be applied to extract chitin. This work provides a suitable strategy for developing an effective method to extract chitin by microbial fermentation.
“…In raw shrimp shells, protein (26.0 ± 0.53%) and ash (47.4 ± 2.2%) content were preferably high ( Table 1 ), which are consistent with the previous studies ( Ghorbel-Bellaaj et al, 2011 ; Hamdi et al, 2017a , b ). The existence of proteins and ash with main components of minerals make shrimp shell hard, which blocks the release of chitin from shrimp shells.…”
Section: Resultssupporting
confidence: 91%
“…Where M O and M R are ash content (%) before and after fermentation; O and R represent the mass (g) of the original sample and fermented residue on a dry weight basis, respectively ( Hamdi et al, 2017a , b ).…”
As an environmentally friendly and efficient method, successive two-step fermentation has been applied for extracting chitin from shrimp shells. To screen out the microorganisms for fermentation, a protease-producing strain, Exiguobacterium profundum, and a lactic acid-producing strain, Lactobacillus acidophilus, were isolated from the traditional fermented shrimp paste. Chitin was extracted by successive two-step fermentation with these two strains, and 85.9 ± 1.2% of protein and 95 ± 3% of minerals were removed. The recovery and yield of chitin were 47.82 and 16.32%, respectively. Fourier transform infrared spectroscopy, X-ray diffraction, and scanning electron microscopy (SEM) were used to characterize the chitin. The crystallinity index was 54.37%, and the degree of deacetylation was 3.67%, which was lower than that of chitin extracted by the chemical method. These results indicated that successive two-step fermentation using these two bacterial strains could be applied to extract chitin. This work provides a suitable strategy for developing an effective method to extract chitin by microbial fermentation.
“…Chitosan (Cs), with acetylation degree of 8%, as characterized by 13 C NMR, molecular weight Mw of 115 kDa and intrinsic viscosity [η] of 3432 ml/g, based on the size exclusion chromatography, was obtained from blue crab shells chitin, through N-deacetylation with NaOH 12.5 M, at a ratio of 1/10 (w/v), for h at °C, as reported in our previous study (Hamdi et al, 2018).…”
Section: Extraction Of Blue Crab Chitosanmentioning
This work aimed to modify blue crab chitosan-based films through the Maillard reaction (MR) as a novel alternative to improve their functional and biological properties. To this end, different saccharides (glucose (aldohexose), fructose (ketohexose), xylose (aldopentose) and arabinose (aldopentose)), at different weight ratios 0.5, 1.0 and 2.0% (g/100 g polymer), were studied, and films were heated at 90 °C for 24 h. Based on color changes and browning index measurements, the extent of MR was the highest with aldopentoses, whereas hexoses and particularly ketohexoses, exhibited a relative crosslinking rate. These findings were further reflected with an improvement in treated films mechanical properties and thermal degradation temperatures, and advantageously, barrier properties against UV light and water. In addition, the MR-modified Cs-based films antioxidant activity was interestingly enhanced with mainly aldopentoses. Consequently, MR crosslinked chitosan-based films are promising alternative for active and functional packaging able of food oxidation hindering, especially using aldopentoses.
“…CD-g-CS exhibited antimicrobial activity against S. xylosus and E. coli as a result of the prominent antimicrobial properties of CS, as reported by Chen et al (2017). Previous studies have also reported the antimicrobial activity of CS against bacteria (Hamdi et al, 2018). The difference in the antibacterial ability of CD-g-CS with Q CD = 0.643 × 10 3 and 0.6 × 10 2 μmol/g may be attributed to the amount of CS.…”
Section: Resultsmentioning
confidence: 92%
“…The amino content of CD-g-CS was determined as described by Hamdi et al (2018) with a few modifications. CD-g-CS (30 mg) was dissolved in 5 mL of HCl (0.1 mol/L).…”
We synthesized chitosan grafted with β-cyclodextrin (CD-g-CS) from mono-6-deoxy-6-(p-toluenesulfonyl)-β-cyclodextrin and chitosan. Two different amounts of immobilized β-cyclodextrin (β-CD) on CD-g-CS (QCD: 0.643 × 103 and 0.6 × 102 μmol/g) were investigated. The results showed that the amino contents of CD-g-CS with QCD = 0.643 × 103 and 0.6 × 102 μmol/g were 6.34 ± 0.072 and 9.41 ± 0.055%, respectively. Agar diffusion bioassay revealed that CD-g-CS (QCD = 0.6 × 102 μmol/g) was more active against Staphylococcus xylosus and Escherichia coli than CD-g-CS (QCD = 0.643 × 103 μmol/g). Cell membrane integrity tests and scanning electron microscopy observation revealed that the antimicrobial activity of CD-g-CS was attributed to membrane disruption and cell lysis. Uptake tests showed that CD-g-CS promoted the uptake of doxorubicin hydrochloride by S. xylosus, particularly for CD-g-CS with QCD = 0.6 × 102 μmol/g, and the effect was concentration dependent. CD-g-CS (QCD = 0.6 × 102 and 0.643 × 103 μmol/g) also improved the aqueous solubilities of sulfadiazine, sulfamonomethoxine, and sulfamethoxazole. These findings provide a clear understanding of CD-g-CS and are of great importance for reducing the dosage of antibiotics and antibiotic residues in animal-derived foods. The results also provide a reliable, direct, and scientific theoretical basis for its wide application in the livestock industry.
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