Development of a Competitive Indirect Enzyme-Linked Immunosorbent Assay for Screening Phenylethanolamine A Residues in Pork Samples

Wang, Xiaomei; Liufu, Tongling; Beloglazova, Natalia V.; Luo, Pengjie; Qu, Jianwen; Jiang, Wenxiao

doi:10.1007/s12161-016-0500-z

Cited by 13 publications

(9 citation statements)

References 29 publications

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“…As depicted in Table , the frit-based immunoassay was able to correctly analyze milk contaminated with AFM 1 or PIR or with both over the cutoff values. The rates for false positive and false negative results were both below 5%, and the specificity and sensitivity rates were >95%, which fulfill the requirements set by the Commission Decision 2002/657/EC for a screening method, − that is, only those analytical techniques, for which it can be demonstrated in a documented, traceable manner that they are validated and have a false compliance rate of <5% at the level of interest shall be used for screening purposes.…”

Section: Resultssupporting

confidence: 61%

A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M₁ and Pirlimycin Residues in Milk

Jiang

Beloglazova

Luo

et al. 2017

J. Agric. Food Chem.

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Mycotoxins and antibacterial agents are the main chemical hazards that lead to several health problems. Nowadays, multiplex immunoassay is a primary goal throughout the world. Here, aflatoxin M and pirlimycin were selected as models, and a novel dual colorimetric encoded frit-based immunoassay was developed for simultaneously screening of aflatoxin M and pirlimycin residues in milk. This multiplex frit-based immunoassay combined two monoclonal antibodies to extend the spectrum of analytes and to enable detection of two classes of analytes in a single test. The cutoff values were 0.02 μg/kg for aflatoxin M and 0.5 μg/kg for pirlimycin, which satisfied the requirement to measure the maximum residue levels. The novel colorimetric frit-based immunoassay has the advantage of high throughput, short analysis time, reduced overall cost per assay, and can be used as a rapid screening technique for simultaneously detecting aflatoxin M and pirlimycin residues in milk.

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Section: Resultssupporting

confidence: 61%

A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M₁ and Pirlimycin Residues in Milk

Jiang

Beloglazova

Luo

et al. 2017

J. Agric. Food Chem.

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“…However, their excessive use in food animals for growth promotion may lead to the presence of their residues in food. The consumption of food contaminated by β‐agonists may cause serious adverse effects, such as food intoxication and cardiovascular and nervous system disorders (Wang et al, 2016). β‐agonists may also increase the risk of acute asthma attacks (Xu et al, 2019).…”

Section: Introductionmentioning

confidence: 99%

“…Despite being prohibited in some countries, these compounds are still being used illegally because they enhance the growth rates in food animals. In recent years, different analytical techniques for the determination of β‐agonist residues in food have been developed to monitor whether these compounds have been misused; these methods include enzyme‐linked immunosorbent assay (ELISA; Wang et al, 2016), GC–MS (Caban, Stepnowski, Kwiatkowski, Migowska, & Kumirska, 2011; Wang, Li, Zhou, & Yang, 2010) and LC–tandem mass spectrometry (LC–MS/MS) (Jia, Chu, Ling, Huang, & Chang, 2014; Kang et al, 2014). These techniques are commonly used for the detection of β‐agonist residues in food.…”

Section: Introductionmentioning

confidence: 99%

The determination of β‐agonist residues in bovine tissues using liquid chromatography–tandem mass spectrometry

Yikilmaz¹,

Kuzukiran

Erdogan³

et al. 2020

Biomedical Chromatography

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We aimed to develop a rapid, simple and reproducible method based on LC–tandem mass spectrometry (LC–MS/MS) to analyze β‐agonist residues (clenbuterol, zilpaterol, ractopamine and isoxsuprine) in bovine tissues. The method was validated in accordance with the European Council Decision 2002/657/EC. The samples were homogenized, and then 10 mL of an acetate buffer was added to a 5‐g sample. The sample was then centrifuged at 12,000 rpm and filtered. Sodium hydroxide (2 m) was added to adjust pH of the sample that was centrifuged again. The extract was filtered through a solid‐phase extraction column. The residue was re‐dissolved in 250 μL acetonitrile and then subjected to LC–MS/MS. The separation was done on a C18 column. The mobile phase consisted of 0.1% formic acid in deionized water and 0.1% formic acid in methanol. The mean recoveries of β‐agonists were in the range of 84.3%–119.1% with relative standard deviations (%RSDs) of 0.683%–4.05%. Decision limits and detection capabilities of the analytes ranged from 0.0960 to 4.9349 μg/kg and from 0.0983 to 5.0715, respectively. This method was used to detect four β‐agonists in 100 bovine muscle, 100 liver and 100 kidney tissues from a slaughterhouse. No residue was found above the maximum residue limit level.

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“…It has several related nerve-stabilizing effects on farm livestock. Intramuscular injection of this drug is used to relieve tension in animals and reduce their activity. − It can make animals indifferent to their environment and keep them in a quiet state for a long term, which is conducive to avoiding fighting when animals live together and in mixed groups. Therefore, it is often used for animals such as pigs during long-distance transportation. − With the widespread application of this drug in veterinary medicine, the problem of its residue in animal tissues and the direct harm to human health caused by its toxic side effects have attracted extensive attention.…”

Section: Introductionmentioning

confidence: 99%

Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver

Lü

Cui

et al. 2020

ACS Omega

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In this study, a highly sensitive and specific monoclonal antibody (mAb) against azaperone was produced. The mAb belonged to the IgG2b subtype and its half maximal inhibitory concentration (IC 50 ) was 0.46 ng/mL. The limit of detection value was 0.081 ng/mL and the linear range (IC 20 −IC 80 ) was 0.165−1.29 ng/mL. Based on this anti-azaperone mAb, a colloidal gold immunochromatographic strip assay method was established for the detection of azaperone in pork and pork liver samples. For pork samples, the cutoff value of the colloidal gold immunochromatographic strip assay was 10 ng/g, and the cutoff value was also 10 ng/g in pig liver samples. This colloidal gold immunochromatographic strip assay is a rapid, simple, and practical method for the detection of azaperone in pork and pork liver samples available in markets.

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Development of a Competitive Indirect Enzyme-Linked Immunosorbent Assay for Screening Phenylethanolamine A Residues in Pork Samples

Cited by 13 publications

References 29 publications

A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M₁ and Pirlimycin Residues in Milk

A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M₁ and Pirlimycin Residues in Milk

The determination of β‐agonist residues in bovine tissues using liquid chromatography–tandem mass spectrometry

Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver

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Development of a Competitive Indirect Enzyme-Linked Immunosorbent Assay for Screening Phenylethanolamine A Residues in Pork Samples

Cited by 13 publications

References 29 publications

A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M1 and Pirlimycin Residues in Milk

A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M1 and Pirlimycin Residues in Milk

The determination of β‐agonist residues in bovine tissues using liquid chromatography–tandem mass spectrometry

Colloidal Gold Immunochromatographic Strip Assay for the Detection of Azaperone in Pork and Pork Liver

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Product

Resources

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A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M₁ and Pirlimycin Residues in Milk

A Dual-Color Quantum Dots Encoded Frit-Based Immunoassay for Visual Detection of Aflatoxin M₁ and Pirlimycin Residues in Milk