Development of a combined technique using a rapid one-step immunochromatographic assay and indirect competitive ELISA for the rapid detection of baicalin

Electrochemical behavior and determination of baicalin on a glassy carbon electrode modified with molybdenum disulfide nano-sheets

“…9B). The detection limit (S/N = 3) was 5.0 × 10 −8 M, which is lower than that reported previously by Paudel and his co-workers by immunochromatographic assay and indirect competitive ELISA method (0.6 μg/mL) [1]. The improved sensitivity could be attributed to that MoS 2 nano-sheets provided not only a large specific surface area to load more baicalin but also an outstanding electric conductivity to accelerate the electron transport, and ultimately resulted in an amplifying electrochemical signal.…”

“…1), as one of the most important bioactive constituent in Scutellariae Radix, has been reported to have anti-inflammatory [3], anti-allergic [4], anxiolytic [5], antioxidant [6], and free-radical scavenging effect [7,8]. Baicalin may also have the potential for treating hepatitis, asthma, HIV, and cancer [1]. In China, the Public Health Department requires that the content of baicalin in Scutellariae Radix should not be b 8.0% in crude drugs [9], and in Japan, the concentration of baicalin defined by the 15th edition of the Japanese pharmacopoeia should be ≥10% (w/w) in Scutellariae Radix for clinical usage [1].…”

“…Baicalin may also have the potential for treating hepatitis, asthma, HIV, and cancer [1]. In China, the Public Health Department requires that the content of baicalin in Scutellariae Radix should not be b 8.0% in crude drugs [9], and in Japan, the concentration of baicalin defined by the 15th edition of the Japanese pharmacopoeia should be ≥10% (w/w) in Scutellariae Radix for clinical usage [1]. Therefore, it is important to establish some accurate, rapid, sensitive and cost-effective methods for the determination of baicalin in traditional medicines.…”

“…Scutellariae Radix, the dried root of the medicinal plant Scutellaria baicalensis Georgi, is a well-known traditional medicine in East Asian countries including China, Japan, Korea and so on [1]. It is one of the most important crude drugs, which widely used in traditional Chinese medicine and Kampo medicines for thousands of years [2].…”

Immunochemical Analysis of the Antimalarial Drugs Artemisinin and Artesunate

“…Immunoassays are convenient and enable the rapid analysis of multiple samples [25][26][27][28][29][30][31][32][33]. We have developed quality control methods for preparing AM from an Artemisia spp.…”