Development of a clinical syndrome resembling haemorrhagic septicaemia in the buffalo following intravenous inoculation of Pasteurella multocida serotype B:2 endotoxin and the role of tumour necrosis factor-α

Horadagoda, N. U.; Hodgson, John; Moon, G.M.; Wijewardana, T.G.; Eckersall, P.D.

doi:10.1053/rvsc.2001.0538

Cited by 49 publications

(38 citation statements)

References 21 publications

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“…Lipopolysaccharide-protein complex (endotoxin) is an important constituent of the outer membrane of a P. multocida cell. Besides its toxic role in pathogenesis of the disease (Schimmel et al, 1982;Mendes et al, 1994;Kunkle and Rimler, 1998;Horadagoda et al, 2002), endotoxin is also immunogenic at low concentrations (Rimler and Phillips, 1986;Muniandy et al, 1998). LPS molecules are not toxic when they are bound to the bacterial outer membrane but after release from the bacterial wall they are exposed to immune cells and evoke an inflammatory response (Van Amersfoort et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Jaglič¹,

Kučerová²,

Nedbalcová³

et al. 2006

Vet. Med. - Czech

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Twenty seven Pasteurella multocida rabbit isolates were characterised by pulsed-field gel electrophoresis (PFGE) generated by restriction endonuclease ApaI, and examined for the presence of capsule and fimbriae, and tested of haemolytic activity and endotoxin release in presence of rabbit plasma. Among the isolates a high number (n = 20) of different PFGE types was observed. All isolates were found to be encapsulated and four of them also possessed fimbriae. Although the presence of the aphA gene, conferring a haemolytic phenotype for Escherichia coli under anaerobic conditions, was indicated in all of the isolates by PCR, clear haemolytic activity was observed in only one isolate when grown anaerobically. All of the isolates grew easily in the presence of rabbit plasma which showed a high capacity to bind the released endotoxin.

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Section: Discussionmentioning

confidence: 99%

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Jaglič¹,

Kučerová²,

Nedbalcová³

et al. 2006

Vet. Med. - Czech

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“…Most studies cease data collection within hours or a few days of LPS injection, which may explain why the present study was the first to demonstrate hyperzincaemia in the late phase of LPS response. A recent study of experimental endotoxin injection in buffalo calves showed hypozincaemia but failed to demonstrate hyperzincaemia, although the animals were observed for 120 h after the challenge [21]. The causes of this discrepancy are not known, but they may be related to differences between breeds or LPS types.…”

Section: Lps-induced Responsesmentioning

confidence: 96%

“…Several previous studies have reported wide inter-individual variation in susceptibility of cattle to systemic LPS challenge as an empirical finding [1,16,21,28,35], but to our knowledge this was the first attempt to statistically quantify the individual differences.…”

Section: Individual Variation In Lps-induced Responsesmentioning

confidence: 99%

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Dose dependency and individual variability in selected clinical, haematological and blood biochemical responses after systemic lipopolysaccharide challenge in cattle

Jacobsen¹,

Toelboell²,

Andersen³

2005

Vet. Res.

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-Previous studies have noted that susceptibility to systemic lipopolysaccharide (LPS) exposure seems to differ between individual cows. However, to date inter-individual variation in the response to intravenous injection of LPS has been reported only as an empirical finding, and its existence or extent has never been backed up by statistical analyses. The aim of the present study was therefore to investigate the dose-dependency of clinical, haematological and blood biochemical responses to intravenous LPS injection in dairy cattle and to determine the extent to which these responses differed between individual cows. Eight dairy cows each received three intravenous injections of Escherichia coli LPS (10, 100, and 1000 ng/kg, consecutively) at three-week intervals. All three LPS doses induced clinical, haematological, and blood biochemical responses lasting up to several days. The strength of all of the responses increased significantly with an increasing LPS dose. A statistically significant inter-individual variation was demonstrated for all clinical, haematological, and blood biochemical responses except for serum calcium concentrations. More than half of the statistical variation in white blood cell and thrombocyte counts could be attributed to the individual. The results of this study show that despite the existence of a dose-response relationship between LPS and ensuing clinical, haematological, and blood biochemical responses, the majority of responses to LPS differ significantly in strength and duration from cow to cow.lipopolysaccharide / susceptibility / dose-response / individual variation

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“…However, there are conflicting reports about the endotoxic properties of LPS isolated from P. multocida. LPS isolated from a serotype B:2 strain was shown to be endotoxic, and intravenously administered LPS could reproduce clinical signs of hemorrhagic septicemia in buffalo (24). However, turkey poults were found to be relatively resistant to the lethal effects of LPS isolated from serogroup A strains of P. multocida, although the inflammatory response and microscopic hepatic lesions were similar to those observed in mammalian hosts (29,34).…”

mentioning

confidence: 89%

A Heptosyltransferase Mutant of Pasteurella multocida Produces a Truncated Lipopolysaccharide Structure and Is Attenuated in Virulence

et al. 2004

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Pasteurella multocida is the causative agent of fowl cholera in birds. In a previous study using signaturetagged mutagenesis, we identified a mutant, AL251, which was attenuated for virulence in mice and in the natural chicken host. Sequence analysis indicated that AL251 had an insertional inactivation of the gene waaQ PM , encoding a putative heptosyl transferase, required for the addition of heptose to lipopolysaccharide (LPS) (M. Harper, J. D. Boyce, I. W. Wilkie, and B. Adler, Infect. Immun. 71:5440-5446, 2003). In the present study, using mass spectrometry and nuclear magnetic resonance, we have confirmed the identity of the enzyme encoded by waaQ PM as a heptosyl transferase III and demonstrated that the predominant LPS glycoforms isolated from this mutant are severely truncated. Complementation experiments demonstrated that providing a functional waaQ PM gene in trans can restore both the LPS to its full length and growth in mice to wild-type levels. Furthermore, we have shown that mutant AL251 is unable to cause fowl cholera in chickens and that the attenuation observed is not due to increased serum sensitivity.Pasteurella multocida is an encapsulated, gram-negative coccobacillus and is the causative agent of a wide range of animal diseases including avian fowl cholera. Several P. multocida virulence factors have previously been identified, including the capsule in serogroups A and B (4, 6), PMT toxin in strains causing atrophic rhinitis in pigs (18), putative filamentous hemagglutinins PfhB1 and PfhB2 (19), and several iron acquisition proteins such as TonB, ExbD and ExbB (3,19,27).During infections by gram-negative bacteria, the presence of lipopolysaccharide (LPS) stimulates the innate immune system whereby the inflammatory response plays a critical role in helping to clear the bacteria and prevent infection. This initial response to gram-negative bacteria can be elicited by a number of bacterial components, the most potent being lipid A, a component of the core structure of LPS. If the inflammatory response in the host is unable to clear the bacteria and the infection is allowed to proceed, the presence of large amounts of systemic LPS can result in endotoxic shock, in which an overproduction of inflammatory mediators causes damage to tissues, septic shock, organ failure, and death (31).LPS is considered to play an important role in the pathogenesis of disease due to P. multocida. Recently it has been shown that LPS from P. multocida assists in adhesion to neutrophils and transmigration through endothelial cells (20). However, there are conflicting reports about the endotoxic properties of LPS isolated from P. multocida. LPS isolated from a serotype B:2 strain was shown to be endotoxic, and intravenously administered LPS could reproduce clinical signs of hemorrhagic septicemia in buffalo (24). However, turkey poults were found to be relatively resistant to the lethal effects of LPS isolated from serogroup A strains of P. multocida, although the inflammatory response and microscopic hepatic lesions we...

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Development of a clinical syndrome resembling haemorrhagic septicaemia in the buffalo following intravenous inoculation of Pasteurella multocida serotype B:2 endotoxin and the role of tumour necrosis factor-α

Cited by 49 publications

References 21 publications

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Characterisation of Pasteurella multocida isolated from rabbits in the Czech Republic

Dose dependency and individual variability in selected clinical, haematological and blood biochemical responses after systemic lipopolysaccharide challenge in cattle

A Heptosyltransferase Mutant of Pasteurella multocida Produces a Truncated Lipopolysaccharide Structure and Is Attenuated in Virulence

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