Development of a clinical laboratory data base of hyper and hypo alpha lipoproteins in Campinas-SP and neighboring region

Abstract

Introduction: The development of research for diagnosis, prevention and treatment of atherosclerotic cardiovascular disease is of utmost importance due to the fact that it is the main cause of morbidity and mortality in Brazil. Objective: To demonstrate the phases of the selection process for candidates with the aim to develop a clinical-laboratorial database of hyper alpha lipoproteinemic patients (hyper A)-high density lipoprotein cholesterol (HDL-C) ≥ 68 mg/dl) and hypo alpha lipoproteinemic patients (hypo A)-HDL-C ≤ 39 mg/dl. Material and methods: The volunteers were contacted after selection of lipid profiles from individuals treated at the Sistema Único de Saúde (SUS), Campinas-SP and neighboring area. Afterwards, the selected patients went through blood collection, clinical examinations and answered questionnaires on dietary frequency and physical activity. After this preliminary evaluation, some individuals were convened to another blood collection and, subsequently, were submitted to an ultrasonographic exam of the carotid arteries. Results: Only 0.6% and 0.3% from 598,288 lipid profiles were selected for hyper A and hypo A groups, respectively, including gender disparity. Lack of effective questionnaires (75%), missing calls (60%) and non-inclusion were the major hindrances in the construction of this database. Discussion: The difficulties to obtain eligible candidates were also due to the low prevalence of both groups hypo A and hyper A and the high prevalence of pathologies that contribute to non-genetic variations of HDL-C. Conclusion: In spite of the obstacles in the development of this database, this study brought about several scientific publications. Furthermore, the development of molecular analyzes and functionality will shortly generate other findings, contributing to the diagnosis and follow-up of HDL dyslipidemias.

Lipase C, Hepatic Type −250A/G (rs2070895) Variant Enhances Carotid Atherosclerosis in Normolipidemic and Asymptomatic Individuals from Brazil

“…Finally, we did not find difference in the physical activity indexes among LIPC rs2070895 genotypes. Curiously, a previous study from our group using a larger number of individuals showed that the physical activity index is significantly higher in individuals with increased levels of HDL‐C (≥68 mg/dL) compared to the group with lower levels of HDL‐C (≤39 mg/dL), even after the adjustment for age, sex, and BMI (Parra et al, ). In addition, in the present study we measured the HDL size using DLS.…”

“…Our study comprised 285 normolipidemic and asymptomatic participants of both genders (51% of females) aged from 19 to 75 years, who underwent free health checkups in primary health care centers during 4 years in Campinas and Americana (São Paulo, Brazil), as previous described by Parra et al () (Fig. ).…”

“…BMI, body mass index; LDL‐C, low‐density lipoprotein cholesterol; n, number of participants; TAG, triacylglycerol. Adapted from Parra et al ()…”

“…During the visit 1 the physical activity indexes were estimated through a questionnaire adapted from Baecke et al () and published by Parra et al (). The physical activity questionnaire is structured by 16 self‐analysis questions, allowing the estimative of three indexes of habitual physical activities in the last 12 months: (1) occupational or labor physical activity index; (2) free time physical activity index, and (3) free time and locomotion activity index.…”

Asymptomatic individuals with high HDL-C levels overexpress ABCA1 and ABCG1 and present miR-33a dysregulation in peripheral blood mononuclear cells

“…Nonsmokers, asymptomatic individuals with body mass index (BMI) lower than 30 kg/m 2 , without regular use of any medications that interfere with lipid metabolism and daily intake of alcohol lower than 14 g, as previously described, were included (Parra et al, 2013).…”