Development of a chicken-derived antivenom against the taipan snake (Oxyuranus scutellatus) venom and comparison with an equine antivenom

Navarro, Diego; Vargas, Mariángela; Herrera, María; Segura, Álvaro; Gómez, Aarón; Villalta, Mauren; Ramírez, Nils; Williams, David J.; Gutiérrez, José Marı́a; León, Guillermo

doi:10.1016/j.toxicon.2016.06.018

Cited by 20 publications

(13 citation statements)

References 52 publications

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“…Our method is more cost-effective than production of horse antivenom, which requires approximately 60 mg for two months. Previous studies also indicated that it is 30–40% less expensive to use chickens as an immunoglobulin source instead of horses [ 31 ]. Based on our results, a strong antibody response was elicited in chickens and lasted for at least six months according to Western blot ( Figure 1 A) and ELISA assays ( Figure 1 B,C).…”

Section: Discussionmentioning

confidence: 99%

Single Chain Antibody Fragment against Venom from the Snake Daboia russelii formosensis

Lee

et al. 2017

Toxins

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Russell’s vipers containing hemotoxic and neurotoxic venom commonly cause snake envenomation. Horse-derived antivenom is a specific antidote, but its production is expensive and has side effects. Developing a cost-effective and more tolerable therapeutic strategy is favorable. In this study, using glutaraldehyde-attenuated Daboia russelii formosensis (DRF) venom proteins to immunize chickens, polyclonal yolk-immunoglobulin (IgY) antibodies were generated and showed a specific binding affinity. Phage display technology was used to generate two antibody libraries of single-chain variable fragments (scFvs) containing 3.4 × 107 and 5.5 × 107 transformants, respectively. Phage-based ELISA indicated that specific clones were enriched after bio-panning. The nucleotide sequences of scFv-expressing clones were analyzed and classified into six groups in the short linker and four groups in the long linker. These scFv antibodies specifically bound to DRF proteins, but not other venom proteins. Mass spectrometric data suggested that these scFv antibodies may recognize phospholipase A2 RV-4 or RV-7. In vivo studies showed that anti-DRF IgY exhibited complete protective effects and mixed scFv antibodies increased the survival rate and time of mice challenged with a lethal dose of DRF proteins. These antibodies can be potentially applied in a rapid diagnostic method or for treatment in the future.

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Section: Discussionmentioning

confidence: 99%

Single Chain Antibody Fragment against Venom from the Snake Daboia russelii formosensis

Lee

et al. 2017

Toxins

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“…However, IgY raised against the venom of the snake Oxyuranus scutellatus was less effective than equine IgG, being unable to neutralize the neurotoxic and coagulant effects of the venom [98]. Nonetheless, this result cannot be extended to IgY produced against other venoms.…”

Section: Anti-venom Activitymentioning

confidence: 92%

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Pereira

Tilburg

Florean

et al. 2019

International Immunopharmacology

124

135

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A B S T R A C TEgg yolk constitutes a relevant alternative source of antibodies. It presents some advantages over mammalian serum immunoglobulins regarding productivity, animal welfare and specificity. The main immunoglobulin present in avian blood (IgY) is transmitted to their offspring and accumulates in egg yolks, which enables the non-invasive harvesting of high amounts of antibodies. Moreover, due to structural differences and phylogenetic distance, IgY is more suitable for diagnostic purposes than mammalian antibodies, since it does not react with certain components of the human immune system and displays greater avidity for mammalian conserved proteins. IgY has been extensively used in health researches, as both therapeutic and diagnostic tool. This article aims to review its applications in both human and veterinary health.

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“…Other studies also concluded that the cost of antibody production was 30%-40% cheaper in hens than horses, especially in developing countries [46]. Numerous studies on IgY used for immunotherapy in clinical and experimental treatments have been reported [24,25].…”

Section: Discussionmentioning

confidence: 99%

Chicken antibodies against venom proteins of Trimeresurus stejnegeri in Taiwan

Lee

Liu

Leu

et al. 2020

J. Venom. Anim. Toxins incl. Trop. Dis

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Background: The venom of bamboo vipers (Trimeresurus stejnegeri-TS), commonly found in Taiwan, contains deadly hemotoxins that cause severe envenomation. Equinederived antivenom is a specific treatment against snakebites, but its production costs are high and there are some inevitable side effects. The aim of the present work is to help in the development of an affordable and more endurable therapeutic strategy for snakebites. Methods: T. stejnegeri venom proteins were inactivated by glutaraldehyde in order to immunize hens for polyclonal immunoglobulin (IgY) antibodies production. After IgY binding assays, two antibody libraries were constructed expressing single-chain variable fragment (scFv) antibodies joined by the short or long linker for use in phage display antibody technology. Four rounds of biopanning were carried out. The selected scFv antibodies were then further tested for their binding activities and neutralization assays to TS proteins. Results: Purified IgY from egg yolk showed the specific binding ability to TS proteins. The dimensions of these two libraries contain 2.4 × 10 7 and 6.8 × 10 7 antibody clones, respectively. An increase in the titers of eluted phage indicated anti-TS clones remarkably enriched after 2 nd panning. The analysis based on the nucleotide sequences of selected scFv clones indicated that seven groups of short linkers and four groups of long linkers were identified. The recombinant scFvs showed significant reactivity to TS venom proteins and a cross-reaction to Trimeresurus mucrosquamatus venom proteins. In in vivo studies, the data demonstrated that anti-TS IgY provided 100% protective effects while combined scFvs augmented partial survival time of mice injected with a lethal amount of TS proteins. Conclusion: Chickens were excellent hosts for the production of neutralization antibodies at low cost. Phage display technology is available for generation of monoclonal antibodies against snake venom proteins. These antibodies could be applied in the development of diagnostic kits or as an alternative for snakebite envenomation treatment in the near future.

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Development of a chicken-derived antivenom against the taipan snake (Oxyuranus scutellatus) venom and comparison with an equine antivenom

Cited by 20 publications

References 52 publications

Single Chain Antibody Fragment against Venom from the Snake Daboia russelii formosensis

Single Chain Antibody Fragment against Venom from the Snake Daboia russelii formosensis

Egg yolk antibodies (IgY) and their applications in human and veterinary health: A review

Chicken antibodies against venom proteins of Trimeresurus stejnegeri in Taiwan

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