Development of a Cheap Media for Bacillus thuringiensis Growth

Hasanain, Amnah M.

doi:10.25141/2475-3432-2017-6.0216

Cited by 3 publications

(2 citation statements)

References 17 publications

(30 reference statements)

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“…Afterwards, the bacteria enter the stationary phase, during which spores and parasporal crystals are produced and released in the culture medium, accompanied by an increase in pH due the consumption of the acetate [34]. In fact, it was previously shown that the optimum for δ-endotoxin production is a culture pH between 6.5 and 7.5, with a slight shift towards an alkaline pH at the end of the sporulation phase, due to cell lysis and release of alkaline metabolites [35]. As per previous experiments, in unbuffered medium, at a pH lower than 6, crystal production is partially inhibited.…”

Section: Response Parameter: δ-Endotoxins Concentrationmentioning

confidence: 99%

Optimization of Culture Conditions and Wheat Bran Class Selection in the Production of Bacillus thuringiensis-Based Biopesticides

et al. 2022

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Bacillus thuringiensis is the leading microbial-based biopesticide, thanks to its parasporal crystal proteins or δ-endotoxins, which are toxic to insect larvae upon ingestion. Once in the insect larvae midgut, the crystal is solubilized by the alkaline pH and the δ-endotoxins activated by proteolytic cleavage. Thanks to its high efficiency as a biopesticide, several efforts have been made to enhance its growth and δ-endotoxins production, in various types of culture media. In this study, a culture medium based on wheat bran (WB), the by-product of cereal grain milling, was used to grow Bacillus thuringiensis and produce δ-endotoxins. Using the response surface methodology (RSM), the effects of three variables were evaluated: WB particles granulometry, their concentration, and their agitation in a 48-h shake-flask culture at 30 °C. Three response parameters were targeted: δ-endotoxins production, final culture pH, and dry-matter consumption. According to the RSM results, the optimum would be at 3.7 g WB/50 mL, with a granulometry above 680 μm and agitation between 170 and 270 rpm. This study is key to developing natural and cheap culture media that can be used at an industrial level for Bacillus thuringiensis-based biopesticides.

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Section: Response Parameter: δ-Endotoxins Concentrationmentioning

confidence: 99%

Optimization of Culture Conditions and Wheat Bran Class Selection in the Production of Bacillus thuringiensis-Based Biopesticides

et al. 2022

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“…Bakteri endofit Bacillus sp. yang ditemukan kemudian dimurnikan dalam medium NA dan diinkubasi pada suhu kamar selama 48 jam (Hasanain, 2017). Jamur R. solani dimurnikan dalam PDA dan diinkubasi selama 7 hari pada suhu kamar (Sharma et al, 2013).…”

Section: Pemurnian Bakteri Endofit Dan Jamur Patogenunclassified

EKSPLORASI DAN UJI VIRULENSI BAKTERI Bacillus sp. ENDOFIT JAGUNG TERHADAP PENYAKIT BUSUK PELEPAH JAGUNG

Saputri

Soesanto

Umayah

et al. 2020

JIPI

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[EXPLORATION AND VIRULENT TEST OF MAIZE ENDOPHYTE Bacillus sp. AGAINST MAIZE SHEATH BLIGHT]. Capability of endophytic bacterial, effectivity, and its effect on R. solani. and on maize seedlings growth were investigated from April 2018 to January 2019. Exploration of endophytes bacteria in maize was taken from Banyumas Regency (Sumbang, Kembaran, Baturraden) and Purbalingga Regency (Padamara, Bojongsari, Pratin). Taking plant samples using Purposive Random Sampling and Diagonal Sampling methods. Completely randomized design was used in in vitro test with 16 treatments repeated twice. Completely randomized block design was used in in planta experiment with 5 treatments repeated 5 times. The treatment consisted of control, fungiside (mankozeb), and 2 isolates of endophytes bacteria performing the best in vitro result. Variables observed included characteristics of endophytic bacteria and pathogenic fungi, inhibition diameter, incubation period, disease intensity, incidence of disease, AUDPC, plant height, leaf number, root length, plant fresh weight, canopy fresh weight, and root fresh weight. Resultshowed that the exploration obtained 15 endophytic Bacillus sp. isolates. The PD A.4 and BK A.1 isolates werw able to inhibit the growth of pathogenic fungi in-vitro by 56.93 and 51.5%, respectively. The soaking treatment using BK A1 was able to reduce disease intensity by 59.377%, and AUDPC value 34.19%. Endophytic bacteria influence plant height, plant fresh weight, canopy fresh weight, and fresh weight of roots respectively as 89.17 cm, 126.06 g, 106.67 g and 19.4 g.

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Optimization of growth media for maximal production of insecticidal vegetative protein (vip3A) from Bacillus thuringiensis and its activity against black cutworm (Agrotis ipsilon)

Alshehrei¹,

El-Ghareeb²,

Baz³

et al. 2021

Afr. J. Biotechnol.

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Bacillus thuringiensis is a Gram-positive bacterium naturally found in soil, water and grain dust. B. thuringiensis has been used for decades as biological control for pests of economical and medical importance. The aim of this study was to test different growth media for the optimal production of vegetative insecticidal protein (Vip3) by B. thuringiensis recovered from the local environment. The local B. thuringiensis was grown under shaker conditions in commercially based media and commonly known growth based media to produce Vip3. The optimal C/N ratio for Vip3 production (3.88) was achieved with 20 g/l of glucose as carbon source and 15 g/l of soybean as nitrogen source. Different combinations of carbon and nitrogen sources were examined. Glucose and soybean were the best carbon and nitrogen source, respectively, for maximal Vip3 production. Terrific Broth supplemented with 20 g/l glucose was found to yield optimum growth significantly (P 0.01, t-test) after 12, 14, 16, 18 and 20 h compared to a formulated medium with optimized C/N ratio of 3.88. The highest vegetative cell count was achieved after 22 h, thus indicating a higher production of Vip3 protein. The method described in this study will facilitate the production of biopesticides in a cost-effective manner.

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Development of a Cheap Media for Bacillus thuringiensis Growth

Cited by 3 publications

References 17 publications

Optimization of Culture Conditions and Wheat Bran Class Selection in the Production of Bacillus thuringiensis-Based Biopesticides

Optimization of Culture Conditions and Wheat Bran Class Selection in the Production of Bacillus thuringiensis-Based Biopesticides

EKSPLORASI DAN UJI VIRULENSI BAKTERI Bacillus sp. ENDOFIT JAGUNG TERHADAP PENYAKIT BUSUK PELEPAH JAGUNG

Optimization of growth media for maximal production of insecticidal vegetative protein (vip3A) from Bacillus thuringiensis and its activity against black cutworm (Agrotis ipsilon)

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