Development of a cell line from primary cultures of rainbow trout, <i>Oncorhynchus mykiss</i> (Walbaum), gills

Bols, Niels C.; Barlian, Anggraini; Chirino‐Trejo, Manuel; Caldwell, Sarah; Goegan, Patrick; Lee, Lucy E. J.

doi:10.1111/j.1365-2761.1994.tb00258.x

Cited by 203 publications

(155 citation statements)

References 18 publications

(22 reference statements)

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“…Establishing permanent cell lines in vitro, either spontaneously or intentionally (Freshney, 2010), makes possible identification of the molecular mechanisms underlying a variety of biological functions, as these cell lines provide a readily available, stable and reproducible system for analyzing identical cells that genetically resemble the original tissues and species (Bols et al, 1994;Smith, 2006). Indeed, cell lines established from insects, amphibians, fish, experimental and livestock animals, as well as humans, have been used in many different types of biological studies such as drug discovery, toxicology, functional studies for genes and proteins, cancer research, and tissue engineering (Smagghe et al, 2009;Lakra et al, 2011;Sinzelle et al, 2012;Stacey, 2012).…”

Section: Introductionmentioning

confidence: 99%

Establishment and Characterization of Permanent Cell Lines from Oryzias dancena Embryos

Lee¹,

Kim²,

Nam³

et al. 2013

Fisheries and aquatic sciences

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The development of species-specific fish cell lines has become a valuable tool for biological research. In recent years, marine medaka Oryzias dancena has been recognized as a good experimental model fish but there are no reports of establishment of cell lines from this fish. In this study, two cell lines from O. dancena blastula embryos were established from 41 total trials (4.9%). The two cell lines displayed typical in vitro morphology and have been cultured for >121 passages, which corresponds to 293 days. The doubling times of the cell lines were 29.84 and 28.59 h, respectively, and both possessed the potential to expand in a clonal manner, albeit with significant differences between the two cell lines. The absence of any of the four main medium supplements; i.e., fish serum, fetal bovine serum, basic fibroblast growth factor, and medaka embryo extract, significantly inhibited growth. The proportion of cells possessing normal chromosome number was 45% and 46.7% of the cell lines, respectively. Taken together, two cell lines that proliferate continuously were established from marine medaka and these cell lines may provide a basic tool for characterizing the unique features of this fish species.

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Section: Introductionmentioning

confidence: 99%

Establishment and Characterization of Permanent Cell Lines from Oryzias dancena Embryos

Lee¹,

Kim²,

Nam³

et al. 2013

Fisheries and aquatic sciences

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“…RTL-W1 cells were maintained under similar conditions as RTgill-W1 but in L-15 containing 5% FBS (v/v). The RTgill-W1 cell line was originally prepared from a histologically normal gill lamella (Bols et al, 1994), whereas the RTL-W1 cells were derived from normal adult trout liver (Lee et al, 1993). The cells were allowed to attach to culture flasks and acclimate for 48 hours prior to chemical exposures.…”

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confidence: 99%

Comparative oxygen radical formation and toxicity of BDE 47 in rainbow trout cell lines

Shao

Eckert

Lee

et al. 2008

Marine Environmental Research

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The polybrominated diphenyl ethers (PBDEs) constitute a class of flame retardants whose residues have markedly increased in fish and human tissues during the last decade. In particular, the levels of certain PBDE congeners in salmon have raised concern regarding potential risks associated with dietary PBDE exposures. However, little is known regarding PBDE-mediated cell injury in relevant in vitro cell models. We conducted a comparative study of oxyradical production and cell injury in rainbow trout gill (RTgill-W1) and trout liver cells (RTL-W1) exposed to 2,2′,4,4′-tetrabromodiphenyl ether (BDE 47), a predominant BDE residue found in fish tissues such as salmonids. Exposure to low micromolar concentrations of BDE 47 elicited a significant loss in RTgill-W1 and RTL-W1 cell viability as measured by alamarBlue assay. The dose-response of BDE toxicity differed among the two cell lines, with the RTL-W1 liver cells showing greater resistance to toxicity at lower BDE 47 doses, but a more dramatic loss of viability relative to gill cells when challenged with higher (50 μM) doses. The sensitivity of the trout liver cells at higher BDE 47 exposures was reflected by a higher basal production of oxygen radical production by 6-carboxy-2′,7′-dichlorodihydrofluorescein diacetate (DCFH-DA) fluorescence that was markedly enhanced in the presence of BDE 47, suggesting an overwhelming of trout liver cell antioxidant defense pathways. Collectively, our data indicate that RTgill-W1 and RTL-W1 liver cells are sensitive to BDE 47-mediated cell injury through a mechanism that may involve oxidative stress. Our data also provide an in vitro basis for potential tissue differences in BDE 47-mediated cell injury.

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“…This epithelial cell line was derived from gill explants of trout (Oncorhynchus mykiss) [25]. According to Lee et al [36] this cell line allows the detailed study of both the cytotoxicity and biotransformation of chemicals that cause cytotoxicity, with a greater efficiency than an in vivo study of the gills.…”

Section: Discussionmentioning

confidence: 99%

In vitro and in vivo toxicity evaluation of the freshwater cyanobacterium Heteroleiblenia kuetzingii

et al. 2013

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Cyanobacteria are prokaryotic organisms characterized by their ability to produce secondary metabolites with different biological activities. The aim of this work was to evaluate the in vitro and in vivo toxicity of the cosmopolitan freshwater cyanobacterium H. kuetzingii. An extract from H. kuetzingii and cyanobacterial growth media were assessed for presence of intracellular and extracellular toxins by in vitro tests using primary cell cultures from mouse kidney and fibroblasts, cell lines A549 and 3T3, a fish cell line RTgill-W1 as well as by a traditional in vivo mouse bioassay. The presence of toxicity was compared with the ELISA and HPLC data for corresponding cyanotoxins. In vitro tests showed pronounced cytotoxicity of the cyanobacterium extract and growth medium in which H. kuetzingii released potential extracellular toxic compounds as the mammalian cells were significantly more sensitive to exposure compared to the fish cells. Histopathological analyses of the liver and kidneys of treated mice showed pathological changes such as leukocyte infiltration and necrosis, changes in the proximal and distal convoluted tubules, lack of differentiation of Bowman's space, enlarged Bowman's capsules and massive hemorrhages. ELISA and HPLC analyses confirmed the presence of saxitoxins and microcystins at low concentrations. In addition, the histological analyses suggest that H. kuetzingii produces other, yet unknown toxic metabolites. Monitoring efforts are therefore required to evaluate the potential hazard for the freshwater aquatic systems and possible public health implications associated with this cyanobacterium.

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Development of a cell line from primary cultures of rainbow trout, Oncorhynchus mykiss (Walbaum), gills

Cited by 203 publications

References 18 publications

Establishment and Characterization of Permanent Cell Lines from Oryzias dancena Embryos

Establishment and Characterization of Permanent Cell Lines from Oryzias dancena Embryos

Comparative oxygen radical formation and toxicity of BDE 47 in rainbow trout cell lines

In vitro and in vivo toxicity evaluation of the freshwater cyanobacterium Heteroleiblenia kuetzingii

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