Development of a cell culture system susceptible to measles, canine distemper, and rinderpest viruses

Key Parameters of Measles Virus Production for Oncolytic Virotherapy

“…Vero cells infected with the TYCSA strain produced a maximum titer of 3×10 6 TCID 50 mL −1 (Shishido et al, 1967), whereas the Leningrad-16 strain amplified in quail embryo cells (Boriskin et al, 1988;Sidorenko et al, 1989), resulted in maximum titers of 1×10 7 or 3.6×10 5 TCID 50 mL −1 (Boriskin et al, 1988). Finally, infection of MRC-5 cells with the AIK-C strain resulted in a maximum titer of 1×10 11 TCID 50 mL −1 (Trabelsi et al, 2010).…”

“…Maximum yields were about 3×10 6 for cell-associated and 5×10 5 TCID 50 mL −1 for released virus. Except for the end of the cultivation period, cell-associated virus titers were always one to two log 10 units higher than extracellular titers (Shishido et al, 1967). Accordingly, released virus in Vero and MA160 cells infected with the Edmonston strain represented only about 10% of the total amount of infectious virus (Baczko and Lazzarini, 1979).…”

“…In some studies MV were also produced at lower temperature (Kohn, 1962;Boriskin et al, 1988;Mendonca et al, 1994;, but MV production at 37°C is common as well (Black, 1959;Shishido et al, 1967, Nakai et al, 1969Romano and Scarlata, 1973;Brandriss et al, 1982). Consequently, the impact of the cultivation temperature during MV production has already been evaluated.…”

“…(Udem, 1984). Shishido et al (1967) infected Vero cells with a variant of the Toyoshima strain; MV titers started to increase for cell-associated virus about 12 h after infection, for extracellular virus about 18 h p.i. Titers decreased after 72 h p.i.…”

“…These are the Hallé strain (Dubois-Dalcq and Reese, 1975;Scott and Choppin, 1982), the TYCSA strain (a variant of the Toyoshima strain) (Shishido et al, 1967), the Leningrad-16 strain (Boriskin et al, 1988;Sidorenko et al, 1989) and the AIK-C strain (Trabelsi et al, 2010) (Table 2).…”