Development of a Capture ELISA for Rapid Detection of Salmonella enterica in Food Samples

Febo, Tiziana Di; Schirone, Maria; Visciano, Pierina; Portanti, Ottavio; Armillotta, Gisella; Persiani, Tiziana; Giannatale, Elisabetta Di; Tittarelli, Manuela; Luciani, Mirella

doi:10.1007/s12161-018-1363-2

Cited by 42 publications

(13 citation statements)

References 29 publications

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“…Recently, immunological methods and molecular biology methods are popular for detecting Salmonella pathogens. Main immunological detection includes ELISA and colloidal gold immunochromatographic test methods, the former detection limit is about 10 3 –10 6 CFU/ml, the detection time is 3–4 hr (Brandao, Liebana, & Pividori, 2015; Di Febo et al, 2019), the latter detection limit is 10 5 CFU/ml, the detection time is about 15 min (Duan et al, 2017); however, Salmonella pathogen often contaminates food only at low levels, which often resulted in negative results. Molecular biology methods have lower detection limits, especially PCR, the detection limit can be up to 10 2 CFU/ml (Alves, Hirooka, & de Oliveira, 2016; Y. J. Li, 2016), the detection time is 5 hr or so, but using the traditional PCR to detect microorganisms often needs extracting DNA, amplification and electrophoresis, and so on, and the operation are relatively complicated.…”

Section: Resultsmentioning

confidence: 99%

Development of a rapid FLISA detection of Salmonella spp. based on CdTe/ZnS quantum dots

Xiang-yang

Liu

2020

Journal of Food Safety

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Salmonella pathogen is old and difficult to control in food safety. The study was aimed to develop a sensitive and rapid CdTe/ZnS quantum dots (QDs) mediated fluorescence-linked immunosorbent assay (FLISA) of Salmonella spp. The optical properties and structure of the CdTe/ZnS QDs were characterized by UV-Vis absorption spectroscopy and transmission electron microscopy. The emission peak wavelength is 575 nm. The CdTe/ZnS QDs-labeled secondary antibody (goat anti-rabbit IgG) was formed by biotin-avidin system. The detection limits of S. Enteritidis and artificial simulated samples are 1.0 × 10 3 CFU/ml and 1.78 × 10 3 CFU/ml, respectively. The study of FLISA stability shows that the antibody with QDs had very good activity when it was stored in the dark at 4 C for at least 35 days. The FLISA provides a new and easy-operated method for the rapid detection of Salmonella spp. and has broad application in food safety.

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Section: Resultsmentioning

confidence: 99%

Development of a rapid FLISA detection of Salmonella spp. based on CdTe/ZnS quantum dots

Xiang-yang

Liu

2020

Journal of Food Safety

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“…In their study, ELISA selectivity and sensitivity increased significantly by increasing the number of antibodies used. Febo et al (2019) applied capture ELISA to detect Salmonella enterica in dairy products, meat, pasta, flour, eggs, and animal feed, and indicated that results by ELISA showed agreement with ISO method with 100, 81, and 90.5% of sensitivity, specificity, and accuracy, respectively. Zhao et al (2020) developed wax‐printed paper‐based ELISA and applied to detect E .…”

Section: Enzyme‐linked Immunosorbent Assaymentioning

confidence: 99%

Bacterial pathogen detection by conventional culture‐based and recent alternative (polymerase chain reaction, isothermal amplification, enzyme linked immunosorbent assay, bacteriophage amplification, and gold nanoparticle aggregation) methods in food samples: A review

Kim

2020

Journal of Food Safety

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The rapid, sensitive, and selective detection of foodborne pathogens is important to ensure food safety. Culture medium‐based methods for bacteria detection have long been used since Robert Koch's first finding. These methods are simple and cheap but have limitations, such as being time‐consuming, labor‐intensive, and having low selectivity. In this regard, several alternative detection methods have been reported. Among these, recent studies related to the application of polymerase chain reaction, isothermal amplification, bacteriophage amplification, enzyme‐linked immunosorbent assay, and gold nanoparticle aggregation for detection of pathogens in food are discussed in this review. The principles, advantages, and disadvantages of alternative methods are covered, including their rapidity, sensitivity, and selectivity. Finally, regulations related to bacterial pathogen detection in the United States and South Korea were compared with remarks for their progress.

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“…At present, quite a lot of attention has been devoted to the research for the rapid detection of E. coli O157:H7 ( Park et al, 2020 ) The conventionally used plate counting method is reliable to some extent but inevitably limited owing to the time-consumption ( Sieuwerts et al, 2008 ; Zhao et al, 2014 ). Technological advances introduced and proposed new methods and techniques, such as polymerase chain reaction (PCR) ( Amagliani et al, 2004 ; Zhou et al, 2022 ) and enzyme-linked immunosorbent assay (ELISA) ( Di Febo et al, 2019 ; Hu Y. et al, 2021 ), but the requirement of high precision and accuracy as well as the need of highly professional trainers limited their use to some extent. To address all these issues related to conventional and advanced techniques, biosensors have been developed ( Aziz et al, 2022 ).…”

Section: Introductionmentioning

confidence: 99%

Boosting the Electrochemical Performance of PI-5-CA/C-SWCNT Nanohybrid for Sensitive Detection of E. coli O157:H7 From the Real Sample

et al. 2022

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Redox activity is an important indicator for evaluating electrochemical biosensors. In this work, we have successfully polymerized indole-5-carboxylic acid into poly-5-carboxyindole nanomaterials (PI-5-CA), using its superior redox activity, and introduced carboxylated single-walled carbon nanotubes (C-SWCNTs) to synthesize a composite material. Finally, a synthesized composite material was used for the modification of the glass carbon electrode to fabricate the PI-5-CA/C-SWCNTs/GCE-based immunosensor and was successfully applied for the sensitive detection of E. coli O157:H7. The fabricated immunosensor exhibited an outstanding electrocatalytic activity toward the detection of E. coli O157:H7 with a remarkably lowest limit of detection (2.5 CFU/ml, LOD = 3 SD/k, n = 3) and has a wide linear range from 2.98×101 to 2.98×107 CFU/ml. Inspired from the excellent results, the fabricated electrode was applied for the detection of bacteria from real samples (water samples) with a good recovery rate (98.13–107.69%) as well as an excellent stability and specificity. Owing to its simple preparation, excellent performance, and detection time within 30 min, our proposed immunosensor will open a new horizon in different fields for the sensitive detection of bacteria from real samples.

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Development of a Capture ELISA for Rapid Detection of Salmonella enterica in Food Samples

Cited by 42 publications

References 29 publications

Development of a rapid FLISA detection of Salmonella spp. based on CdTe/ZnS quantum dots

Development of a rapid FLISA detection of Salmonella spp. based on CdTe/ZnS quantum dots

Bacterial pathogen detection by conventional culture‐based and recent alternative (polymerase chain reaction, isothermal amplification, enzyme linked immunosorbent assay, bacteriophage amplification, and gold nanoparticle aggregation) methods in food samples: A review

Boosting the Electrochemical Performance of PI-5-CA/C-SWCNT Nanohybrid for Sensitive Detection of E. coli O157:H7 From the Real Sample

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