Development of a capture ELISA to determine kinetics of soluble CD25 following in vitro and in vivo stimulation of duck peripheral blood monocytes

Huang, Zhenyu; Fang, Jie; Gu, Jianyou; Yan, Yan; Zhou, Jiyong

doi:10.1016/j.vetimm.2010.11.021

Cited by 8 publications

(3 citation statements)

References 47 publications

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“…Chicken sera were diluted 1:2 in PBS-T, 100 μl added to the wells and incubated for 2 h at room temperature. The plates were washed with PBS-T, and 100 μl/well of peroxidase-conjugated rabbit anti-α-toxin or NetB toxin antibodies were added and incubated for 30 min followed by development with 3,3′,5,5′-tetramethylbenzidine substrate as described previously ( 26 , 27 ) . Optical densities at 450 nm were measured and serum α-toxin or NetB toxin concentrations were determined using a standard curve generated with known concentrations of each purified recombinant protein.…”

Section: Methodsmentioning

confidence: 99%

Dietary supplementation of young broiler chickens with Capsicum and turmeric oleoresins increases resistance to necrotic enteritis

et al. 2013

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The Clostridium-related poultry disease, necrotic enteritis (NE), causes substantial economic losses on a global scale. In the present study, a mixture of two plant-derived phytonutrients, Capsicum oleoresin and turmeric oleoresin (XT), was evaluated for its effects on local and systemic immune responses using a co-infection model of experimental NE in commercial broilers. Chickens were fed from hatch with a diet supplemented with XT, or with a non-supplemented control diet, and either uninfected or orally challenged with virulent Eimeria maxima oocysts at 14 d and Clostridium perfringens at 18 d of age. Parameters of protective immunity were as follows: (1) body weight; (2) gut lesions; (3) serum levels of C. perfringens a-toxin and NE B-like (NetB) toxin; (4) serum levels of antibodies to a-toxin and NetB toxin; (5) levels of gene transcripts encoding pro-inflammatory cytokines and chemokines in the intestine and spleen. Infected chickens fed the XT-supplemented diet had increased body weight and reduced gut lesion scores compared with infected birds given the non-supplemented diet. The XT-fed group also displayed decreased serum a-toxin levels and reduced intestinal IL-8, lipopolysaccharide-induced TNF-a factor (LITAF), IL-17A and IL-17F mRNA levels, while cytokine/chemokine levels in splenocytes increased in the XT-fed group, compared with the animals fed the control diet. In conclusion, the present study documents the molecular and cellular immune changes following dietary supplementation with extracts of Capsicum and turmeric that may be relevant to protective immunity against avian NE.

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Section: Methodsmentioning

confidence: 99%

Dietary supplementation of young broiler chickens with Capsicum and turmeric oleoresins increases resistance to necrotic enteritis

et al. 2013

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“…The symptoms are similar to clinical signs infected other bacteria in ducks. Vaccine could be used to control R. anatipestifer infection ( Liu et al., 2013 ; Chu et al., 2015 ), and antibody level was used to evaluate the effect of immunization with ELISA ( Huang et al., 2011 ). It is difficult to distinct antibody levels in vaccinated and wild-type R. anatipestifer- infected ducks.…”

Section: Discussionmentioning

confidence: 99%

Development of a colloidal gold immunochromatographic strip for rapid detection of Riemerella anatipestifer in ducks

et al. 2020

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Riemerella anatipestifer is one of the major bacterial pathogens of ducks and causes significant economic losses in poultry agriculture. Usually, methods for detecting R. anatipestifer infection need specialized equipment and highly skilled personnel. In this study, a novel colloidal gold immunochromatographic strip was developed for rapid detection of R. anatipestifer in ducks. The monoclonal antibodies 2D5 and 2A6 against R. anatipestifer were used as colloidal gold-labeled protein and capture protein, respectively, to recognize the bacteria in tryptic soy broth medium culture and in hearts of infected ducks. The goat anti-mouse IgG antibody was labeled on nitrocellulose membrane as a control for C line. The labeling pH was optimized as 10.0, and the concentration of 2D5 labeled to colloidal gold particles was optimized as 18 μg/mL. The strip specifically detected serotypes 1, 2, and 10 R. anatipestifer strains and showed no cross-reaction with Escherichia coli , Salmonella enterica, and Pasteurella multocida strains. The sensitivity of the strip for detecting R. anatipestifer was 1.0 × 10 6 colony forming unit. The strips remained stable for up to 8 mo at 4°C, and the detection can be completed within 15 min. The strip can detect R. anatipestifer in hearts of the ducks experimentally infected with R. anatipestifer but not infected with E. coli , which were also confirmed with bacterial isolation followed by multiplex polymerase chain reaction. These results suggested that the strips are reliable methods for identification of R. anatipestifer in laboratories and in duck farms.

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“…Horseradish peroxidase-labeled goat anti-duck IgY (Luoyang Bai Aotong Experimental Materials Center, Henan, China) was used as a secondary antibody to measure serum IgY antibody levels in immunized ducks. Incubation with the secondary antibody was performed for 1 h at 37°C ( Huang et al, 2002 ; Lobbedey and Schlatterer, 2003 ; Jia et al, 2009 ; Huang et al, 2011 ).…”

Section: Methodsmentioning

confidence: 99%

Immunogenicity of live phoP gene deletion strain of Riemerella anatipestifer serotype 1

Li¹,

Zhang²,

Wang³

et al. 2023

Poultry Science

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Development of a capture ELISA to determine kinetics of soluble CD25 following in vitro and in vivo stimulation of duck peripheral blood monocytes

Cited by 8 publications

References 47 publications

Dietary supplementation of young broiler chickens with Capsicum and turmeric oleoresins increases resistance to necrotic enteritis

Dietary supplementation of young broiler chickens with Capsicum and turmeric oleoresins increases resistance to necrotic enteritis

Development of a colloidal gold immunochromatographic strip for rapid detection of Riemerella anatipestifer in ducks

Immunogenicity of live phoP gene deletion strain of Riemerella anatipestifer serotype 1

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