Development of a capillary electrophoresis assay for the determination of carvedilol enantiomers in serum using cyclodextrins

Clohs, Lilian; McErlane, Keith M.

doi:10.1016/s0731-7085(00)00468-4

Cited by 50 publications

(37 citation statements)

References 35 publications

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“…In the past two decades, CE has emerged as a powerful and versatile separation tool due to its high sensitivity, resolution, and ability to detect minute quantities of samples [1][2][3][4][5][6][7][8][9][10][11]. CE differentiates charged species on the basis of mobility differences under the influence of an applied electric field.…”

Section: Introductionmentioning

confidence: 99%

Response surface examination of the relationship between experimental conditions and product distribution in electrophoretically mediated microanalysis

2008

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This work presents the first known use of response surface methodology (RSM) in electrophoretically mediated microanalysis. This concept is demonstrated by examining the optimization of reaction conditions for the conversion of nicotinamide adenine dinucleotide to nicotinamide adenine dinucleotide, reduced form by glucose-6-phosphate dehydrogenase (G6PDH, EC 1.1.1.49) in the conversion of glucose-6-phosphate to 6-phosphogluconate. Experimental factors including voltage, enzyme concentration, and mixing time of reaction at the applied voltage were selected at three levels and tested in a Box-Behnken response surface design. Upon migration in a capillary under CE conditions, plugs of substrate and enzyme are injected separately in buffer and allowed to react at variable conditions. Extent of reaction and product ratios were subsequently determined by CE. The model predicted results are shown to be in good agreement (7.1% discrepancy difference) with experimental data. The use of chemometric RSM provides a direct relationship between electrophoretic conditions and product distribution of microscale reactions using CE, thereby offering a new and versatile approach to optimizing enzymatic experimental conditions.

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Section: Introductionmentioning

confidence: 99%

Response surface examination of the relationship between experimental conditions and product distribution in electrophoretically mediated microanalysis

2008

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“…HPLC methods using a chiral stationary phase have been reported for the analysis of carvedilol enantiomers 6,7 . The clean-up procedures for the extraction of carvedilol from biological matrix consist of protein precipitation 5,6,10,12 , solid-phase extraction (SPE) 2,3,9 , liquid-liquid extraction (LLE) 6,7,[11][12][13] , combinations of protein precipitation with SPE 4 or combinations of LLE with back-extraction 1,8 . Those methods use a large amount of biological samples (0.15-1.0 mL plasma or 2-5 mL urine samples) in order to obtain the high sensitivity or include time-consuming extraction procedures and/or relatively long run time.…”

Section: Introductionmentioning

confidence: 99%

Analysis of Carvedilol and Its Metabolite in Human Plasma Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

Janjanam¹,

Bimireddy²

2017

IJPTR

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: A rapid, sensitive and selective method for the determination of Carvedilol and its metabolite in human plasma was developed using liquid chromatography with tandem mass spectrometry (HPLC-MS/MS). Carvedilol, its metabolite 4-OH carvedilol and abacavir (internal standard) were extracted from human plasma by solid phase extraction technique (SPE) and analyzed on an Discovery column (C8, 50 × 4.6 mm, 5µ) with the mobile phase of acetonitrile -0.1% formic acid in water (70:30 v/v). The analytes were detected using an electro spray ionization tandem mass spectrometry in the multiple reaction monitoring mode. The standard curve was linear (r = 0.9998) over the concentration range of 0.5-100 ng/mL and 0.3-40 ng/mL for carvedilol and 4-OH carvedilol respectively. The lower limit of quantification for carvedilol was 0.5 ng/mL and 0.3 ng/mL for carvedilol and 4-OH carvedilol respectively using 500 µL plasma samples. The coefficient of variation and relative error for intra-and inter-assay at four QC levels were 3.37 to 9.53 %, 4.76 to 7.01% and 3.31 to 6.91%, 5.23 to 6.64 % respectively. The matrix effect for carvedilol, 4-OH carvedilol and abacavir were practically absent. The extraction recoveries of carvedilol, 4-OH carvedilol and abacavir were 78.90, 83.25 and 85.20%, respectively.

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“…Different columns such as Phenomenex 3022 (Torrance, CA) [14], Chiralpak AD (Daicel Chemical Industries, Tokyo, Japan) [15], and Chiralcel OD-RH (Chiral Technologies Europe, France) [16] coupled to an HPLC system with fluorescence detection have been described for the analysis of Carvedilol enantiomers in plasma. Capillary electrophoresis using hydroxypropyl-beta-cyclodextrin as chiral selector has also been used for the enantioselective analysis of Carvedilol in serum using ultraviolet detector [17], or laser-induced fluorescence detector [18].…”

Section: Introductionmentioning

confidence: 99%

HPLC Method Development and Validation of S(-)-Carvedilol from API and Formulations

Swetha¹,

Chandupatla²,

Veeresham³

2015

AJAC

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A simple chiral HPLC method was developed and validated for quantification of S(-)-Carvedilol in Active Pharmaceutical Ingredient (API) and marketed tablet formulation of racemic Carvedilol. Chiral resolution of enantiomers of Carvedilol was achieved on Phenomenex Lux-cellulose-4 (250 mm × 4.6 mm; 5 µ particle size) chiral column by using a mobile phase, Isopropanol and n-Heptane (60:40 v/v), at a flow rate of 1.0 ml/min and by employing UV detection at 254 nm wavelength. The method was validated according to the ICH guidelines and was proved to be specific, linear, precise and accurate for the analysis of S(-)-Carvedilol.

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Development of a capillary electrophoresis assay for the determination of carvedilol enantiomers in serum using cyclodextrins

Cited by 50 publications

References 35 publications

Response surface examination of the relationship between experimental conditions and product distribution in electrophoretically mediated microanalysis

Response surface examination of the relationship between experimental conditions and product distribution in electrophoretically mediated microanalysis

Analysis of Carvedilol and Its Metabolite in Human Plasma Using Liquid Chromatography Coupled with Tandem Mass Spectrometry

HPLC Method Development and Validation of S(-)-Carvedilol from API and Formulations

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