Development of a Candidate Reference Measurement Procedure for the Analysis of Cortisol in Human Serum Samples by Isotope Dilution-Gas Chromatography-Mass Spectrometry
Abstract:A candidate reference measurement procedure involving isotope dilution coupled with gas chromatography-mass spectrometry (GC-MS) has been developed and critically evaluated. An isotopically labeled internal standard, cortisol-d2, was added to a serum sample. After equilibration, solid-phase extractions (SPE) for sample preparation and derivatization with heptafluorobutyric anhydride (HFBA) were performed for GC-MS analysis. The limit of detection (LOD) and the limit of quantification (LOQ) were 5 and 20 ng g -… Show more
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