Development of a CAFs-related gene signature to predict survival and drug response in bladder cancer

Zhang, Zhao; Liang, Zhijuan; Li, Dan; Wang, Liping; Chen, Yuanbin; Liang, Ye; Jiao, Wei; Niu, Haitao

doi:10.1007/s13577-022-00673-w

Cited by 14 publications

(8 citation statements)

References 43 publications

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“…Recently, molecular biology and genetic technology have played an important role in the diagnosis and prognosis of metastasis bladder cancer. Highly expressed CD164, CD133 + , CD24 + , and cafs genes were associated with metastasis and invasion of bladder cancer[ 22 – 24 ]. Overexpression of SOX30 inhibits the proliferation, invasion, and migration of bladder cancer[ 25 ].…”

Section: Discussionmentioning

confidence: 99%

Constructing and validating nomograms to predict risk and prognostic factors of distant metastasis in urothelial bladder cancer patients: a population-based retrospective study

Chen

Luo

et al. 2022

BMC Urol

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Background Urothelial carcinoma is the most common type of bladder cancer worldwide and it has a poor prognosis for patients with distant metastasis. Nomograms are frequently used in clinical research, but no research has evaluated the diagnostic and prognostic factors of distant metastasis in urothelial bladder cancer (UBC). Methods The Surveillance, Epidemiology, and End Results database was used to analyze all patients diagnosed with UBC between 2000 and 2017. Lasso regression was used to identify the potential risk predictive factors for distant metastasis in UBC. Univariate and multivariate Cox proportional hazard regression analyses were performed to determine independent prognostic factors for distant metastasis urothelial bladder cancer (DMUBC). Subsequently, two nomograms were constructed based on the above models. The receiver operating characteristic (ROC), and calibration curves were performed to evaluate the two nomograms. Results The study included 73,264 patients with UBC, with 2,129 (2.9%) having distant metastasis at the time of diagnosis. In the diagnostic model, tumor size, histologic type, and stage N and T were all important risk predictive factors for distant metastasis of UBC. In the prognostic model, age, tumor size, surgery, and chemotherapy were independent factors affecting the prognosis of DMUBC. DCA, ROC, calibration, and Kaplan–Meier (K–M) survival curves reveal that the two nomograms can effectively predict the diagnosis and prognosis of DMUBC. Conclusion The developed nomograms are practical methods for predicting the occurrence risk and prognosis of distant metastasis urothelial bladder cancer patients, which may benefit the clinical decision-making process.

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Section: Discussionmentioning

confidence: 99%

Constructing and validating nomograms to predict risk and prognostic factors of distant metastasis in urothelial bladder cancer patients: a population-based retrospective study

Chen

Luo

et al. 2022

BMC Urol

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“…APQ3 expression is strongly related to skin epidermal homeostasis and wound healing [ 36 ]. IVL encodes involucrin, which is essential for skin barrier formation and integrity [ 37 ]. Thus, our findings suggest that the reduction in EDAR expression can lead to the impairment of wound healing and that controlling EDAR expression can be a potential wound-healing strategy.…”

Section: Resultsmentioning

confidence: 99%

Fine Wrinkle Improvement through Bioactive Materials That Modulate EDAR and BNC2 Gene Expression

Lee,

Ye,

Kim

et al. 2024

Biomolecules

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Skin aging is a multifaceted biological phenomenon influenced by a combination of intrinsic or extrinsic factors. There is an increasing interest in anti-aging materials including components that improve skin wrinkles. Despite the availability of several such wrinkle-improving materials, the demand for ingredients with outstanding efficacy is increasing. Therefore, this study aimed to explore the mechanisms of wrinkle-related genes reported in previous genome-wide association studies (GWASs), identify materials that regulate these genes, and develop an effective anti-wrinkle formula containing the active ingredients that regulate the expression of these genes. We selected two candidate genes, EDAR and BNC2, that are reportedly related to periorbital wrinkles. We investigated their functions in the skin through in vitro experiments using human skin cell lines (keratinocytes and fibroblasts). Moreover, we identified ingredients that regulate the expression of these two genes and confirmed their efficacy through in vitro experiments using the skin cell lines. Finally, we developed a formula containing these ingredients and confirmed that it enhanced dermal collagen in the 3D skin and improved fine wrinkles under the eyes more effectively than retinol in humans, when applied for 8 weeks. Our results are significant and relevant, as we have discovered a special formula for wrinkle improvement with reliable efficacy that surpasses the efficacy of retinol and does not cause side-effects such as skin irritation.

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“…High expression of EFEMP1 was significantly associated with high pathological stage, high histological grade, poor prognosis and lymph node metastasis of BLCA ( Chen et al, 2021 ). It has been reported that LAMA2 is not only associated with the prognosis of bladder cancer, but also strongly related to drug sensitivity ( Gu et al, 2022 ; Zhang et al, 2022b ). A study showed that SLC1A6 expression levels in BLCA correlated with the proportion of T cells and neutrophils in the TIME and is an unfavorable prognostic factor for patients (27).…”

Section: Discussionmentioning

confidence: 99%

Uncovering the potential functions of lymph node metastasis-associated aberrant methylation differentially expressed genes and their association with the immune infiltration and prognosis in bladder urothelial carcinoma

Gao¹,

Zhang²,

Tian³

et al. 2023

PeerJ

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Background Bladder urothelial carcinoma (BLCA) is a malignant tumor of the urinary system. This study aimed to explore the potential role of lymph node metastasis-associated aberrant methylation differentially expressed genes (DEGs) in BLCA. Methods CHAMP and limma packages were used to identify lymph node metastasis-associated aberrant methylation DEGs. Univariate Cox analysis and Lasso analysis were performed to identify the signature genes, and multivariate Cox analysis was used to construct the risk score. Subsequently, the molecular characteristics of the signature genes and the relationship between risk score and prognosis, clinical characteristics and immune cell infiltration were analyzed. The signature gene AKAP7 was selected for functional verification. Results A novel risk score model was constructed based on 12 signature genes. The risk score had a good ability to predict overall survival (OS). The nomogram constructed based on age, N stage and risk score had a higher value in predicting the prognosis of patients. It was also found that stromal activation in TIME may inhibit the antitumor effects of immune cells. Functional enrichment analysis revealed that ECM receptor interaction and focal adhesion were two important pathways involved in the regulation of BLCA. Immunohistochemistry showed that AKAP7 may be associated with the occurrence, clinical stages and grades, and lymph node metastasis of BLCA. In vitro cell experiments showed that the migration and invasion ability of EJ cells was significantly inhibited after AKAP7 overexpression, while the migration and invasion ability of T24 cells was significantly promoted after AKAP7 knockdown. Conclusion The risk score model based on lymph node metastasis-associated aberrant methylation DEGs has a good ability to predict OS and is an independent prognostic factor for BLCA. It was also found that stromal activation in TIME may inhibit the antitumor effects of immune cells. This implicates aberrant methylation modifications as an important factor contributing to the heterogeneity and complexity of individual tumor microenvironments. Functional enrichment analysis revealed that ECM receptor interaction and focal adhesion were two important pathways involved in the regulation of BLCA, which contributed to the exploration of the pathological mechanism of BLCA. In addition, immunohistochemistry showed that AKAP7 may be associated with the occurrence, progression and lymph node metastasis of BLCA. In vitro cell experiments showed that AKAP7 could also inhibit the migration and invasion of cancer cells.

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Development of a CAFs-related gene signature to predict survival and drug response in bladder cancer

Cited by 14 publications

References 43 publications

Constructing and validating nomograms to predict risk and prognostic factors of distant metastasis in urothelial bladder cancer patients: a population-based retrospective study

Constructing and validating nomograms to predict risk and prognostic factors of distant metastasis in urothelial bladder cancer patients: a population-based retrospective study

Fine Wrinkle Improvement through Bioactive Materials That Modulate EDAR and BNC2 Gene Expression

Uncovering the potential functions of lymph node metastasis-associated aberrant methylation differentially expressed genes and their association with the immune infiltration and prognosis in bladder urothelial carcinoma

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