Development of a broad-spectrum monoclonal antibody-based indirect competitive enzyme-linked immunosorbent assay for screening of androgens in animal edible tissues

Homologous ELISA for detection of 17α-methyltestosterone in serum

“…There has been no ELISA development reported for the detection of 17α-methyltestosterone in India till date. However, antigen based indirect competitive ELISAs have been developed in Ireland, China and Czech Republic (Prague) (H. Gao et al, 2021;Huml et al, 2020;Kong et al, 2015;Lu et al, 2006) but they have some limitations like every time antibody will be required to generate for the assay which is time consuming and variations are also likely to occur in binding of the enzyme conjugate. The sensitivity (least detectable dose) and ED50 of our developed assay were calculated as 0.11 ng/ml and 5.78 ng/ml respectively.…”

“…Physical methods presently available are High resolution orbitrap MS (Vanhaecke et al, 2013), LC-MS (Gonzalo-Lumbreras & Izquierdo-Hornillos, 2003), LC-MS/MS (Chu et al, 2006;Kaklamanos et al, 2007) LC-MS combined with online turbulent flow extraction (Du Shin et al, 2017), LC-HRMS (Tudela et al, 2015); HPLC (Cravedi & Delous, 1991;Han et al, 2012;Marwah et al, 2005;Shi et al, 2008), HPLC-MS/MS (Regal et al, 2010), Ultra-HPLC with Orbitrap-high resolution MS (López-García et al, 2018); HPLC combined with RIA (Daeseleire et al, 1991), HPLC-UV (Li et al, 2020), GC-MS (Bi & Massé, 1992;Shinohara et al, 1985;Zakhari et al, 1991;Zeng et al, 2010), GC-MS/MS (Wong et al, 2017), GCMS-MIPFs-OCDD-LVI (Zhong et al, 2013), Gas chromatography microchip APPI-MS/MS (Hintikka et al, 2013), hollow fiber solvent-stir bar micro extraction (HF-SSBME) device (Liu et al, 2012); SFE-SPE combined with GC-MS (Stolker et al, 1999); New mixed surfactant MEKC method (Zhang et al, 2009); Fluorescence modulation method (Haynes & Levine, 2020), Arrayed based sensing (Gill et al, 2019) and Thin layer chromatography (Musharraf et al, 2017) for the analysis of 17αmethyltestosterone. Immunological methods such as RIA (Hampl et al, 1978), CLIA (Van Peteghem et al, 1989); (Jansen et al, 1985;Xie et al, 2005), ELISA (H. Gao et al, 2021;Huml et al, 2020;Kong et al, 2015;Lu et al, 2006;…”