Development of a black gram [Vigna mungo (L.) Hepper] linkage map and its comparison with an azuki bean [Vigna angularis (Willd.) Ohwi and Ohashi] linkage map

Abstract

The Asian Vigna group of grain legumes consists of six domesticated species, among them black gram is widely grown in South Asia and to a lesser extent in Southeast Asia. We report the first genetic linkage map of black gram [Vigna mungo (L.) Hepper], constructed using a BC(1)F(1) population consisting of 180 individuals. The BC(1)F(1) population was analyzed in 61 SSR primer pairs, 56 RFLP probes, 27 AFLP loci and 1 morphological marker. About 148 marker loci could be assigned to the 11 linkage groups, which correspond to the haploid chromosome number of black gram. The linkage groups cover a total of 783 cM of the black gram genome. The number of markers per linkage group ranges from 6 to 23. The average distance between adjacent markers varied from 3.5 to 9.3 cM. The results of comparative genome mapping between black gram and azuki bean show that the linkage order of markers is highly conserved. However, inversions, insertions, deletions/duplications and a translocation were detected between the black gram and azuki bean linkage maps. The marker order on parts of linkage groups 1, 2 and 5 is reversed between the two species. One region on black gram linkage group 10 appears to correspond to part of azuki bean linkage group 1. The present study suggests that the azuki bean SSR markers can be widely used for Asian Vigna species and the black gram genetic linkage map will assist in improvement of this crop.

Development of new gene-specific markers associated with salt tolerance for mungbean (Vigna radiata L. Wilczek)

“…All microsatellite markers used in this study showed considerable amplif ication and highly signif icant polymorphism (86.84-100%) within the analyzed genotypes from different Vigna species. The obtained polymorphism is significantly higher as compared to that reported earlier (Chaitieng, 2006;Somta et al, 2009;Sudha et al, 2012). The SSR markers were developed from the candidate gene Glycine max, which is involved in the SOS pathway.…”

Reciprocal translocation identified in Vigna angularis dominates the wild population in East Japan

“…This was unexpected, considering the original structures of chromosomes are well conserved in many Asian Vigna species including cultivated and wild accessions (Han et al 2005;Chaiteng et al 2006;Isemura et al 2010Isemura et al , 2012Somta et al 2006). Thus, since all the cultivated azuki beans and the wild accessions from West Japan retained the original chromosome structures of Asian Vigna species, the translocation-type chromosomes had probably emerged once, and only in the wild population in East Japan.…”

“…Since the first linkage map had the same structure as those we had constructed using the common DNA marker set, such as rice bean (V. umbellata) (Isemura et al 2010), mungbean (V. radiata) (Isemura et al 2012), black gram (V. mungo) (Chaiteng et al 2006), and other related Vigna species (Somta et al 2006), Kaga et al concluded that the first linkage map indicated the original chromosomal structure and the second linkage map indicated the presumed translocation in either of the parental lines; JP110658 or ' KyotoDainagon' (2008).…”

Application of SSR markers and real-time PCR for variety identification in azuki-bean [Vigna angularis (Willd.) Ohwi and Ohashi]

“…In addition, this technique has been used for genetic analysis and crop breeding program based on marker assisted selection (Powell et al 1996). SSR markers in azuki bean were developed and analyzed for gene flow studies and linkage map construction in Japan (Han et al 2005;Wang et al 2004;Chaitieng et al 2006).…”