Development of a biosensor for gaseous cyanide in solution

Lee, Jeong Im; Karube, Isao

doi:10.1016/0956-5663(96)82337-2

Cited by 22 publications

(5 citation statements)

References 11 publications

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“…The cyanate is then hydrolyzed by cyanase, and ammonia and carbon dioxide are produced. By use of this mechanism of microbial degradation of cyanide several biosensors were developed with a batch system [264] or with flow systems [265,266]. To develop a cyanide-specific biosensor we the developed a biosensor using the rhodanese-sulfite oxidase reaction and subsequent luminol CL reaction combined with an FIA (CL-FIA) system [267].…”

Section: Toxin Sensorsmentioning

confidence: 99%

Current research activity in biosensors

Nakamura

Karube

2003

Analytical and Bioanalytical Chemistry

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129

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Biosensors consist of a molecular recognition element and a transducer. Since the first biosensor was developed by Updike and Hicks many biosensors and their associated techniques have been studied and developed. In this review current research activity on the fundamentals and applications of biosensors is summarized. In discussion of the former, molecular recognition elements, techniques and tools for biosensor construction, and basic biosensor devices are introduced. Coverage of the latter includes description of biosensors for environmental, food, and clinical fields. Chemical sensors developed in our laboratory for environmental monitoring are also described. This review mainly summarizes work performed by our group and by our colleagues, and refers to the main review articles summarizing each field of biosensor research in recent years.

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Section: Toxin Sensorsmentioning

confidence: 99%

Current research activity in biosensors

Nakamura

Karube

2003

Analytical and Bioanalytical Chemistry

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259

129

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“…Cyanide biosensors were thus developed by inhibition of enzymes: horseradish peroxidase − polyphenol oxidase − cytochrome oxidase and microorganisms ( Pseudomonas fluorescens NCIMB 11764), , allowing with the most sensitive configuration to detect cyanide down to ∼0.02 μM.…”

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confidence: 99%

Subnanomolar Cyanide Detection at Polyphenol Oxidase/Clay Biosensors

2003

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A novel, inexpensive, and simple amperometric biosensor based on immobilization of polyphenol oxidase (PPO) into Zn-Al layered double hydroxides, also called anionic clays, is applied for determination of cyanide. The detection of cyanide was performed via its inhibiting action on the PPO electrode. Measurement was carried out with 3,4-dihydroxyphenylacetic acid as enzyme substrate, the enzymatically generated quinoid products being electroreduced at -0.2 V. An extremely sensitive detection limit (0.1 nM) was obtained for cyanide. Enzyme immobilization into an anionic exchanger clay seems to cause an increase in cyanide inhibition effects because of anion accumulation in the clay matrix.

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“…HClO + H7 + 2 e -+ C1' + HzO (2) The dependence of the biosensor response on hydrogen peroxide and halide concentration represents the kinetics of haloperoxidase as well as mass transport where the kinetic parameters a and P are related to enzymatic reaction rate. may be perturbed by the pH.…”

Section: Biosensor Response Measurementmentioning

confidence: 99%

The Determination of Halides and Pseudohalides by the Vanadium Haloperoxidase Based Biosensor

Kulys

Vidžiūnaitė

1998

Analytical Letters

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A biosensor containing vanadium haloperoxidase (VHP) immobilized on a graphite electrode responded to the halides and hydrogen peroxide at electrode potentials from -0.1 to 0.25 V vs SCE. An apparent Michaelis-Menten constant (K,) of the biosensor was 12-14 pM for hydrogen peroxide at 0.1-0.2 mM of KCI and 0.1 mh4 of sodium vanadate. K, decreased up to 2.3 pM in a vanadate fiee solution. At the same conditions and at a fixed concentration (0.1 mM) of hydrogen peroxide K, for chloride changed from 0.6 to 2.5 mM. K,, for bromide was 9-16 p M and for iodide it was 5.5 pM. The biosensor response was largest at pH 5.1-5.3 and at pH 6.1 in presence of chloride and iodide, respectively. 2607

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Development of a biosensor for gaseous cyanide in solution

Cited by 22 publications

References 11 publications

Current research activity in biosensors

Current research activity in biosensors

Subnanomolar Cyanide Detection at Polyphenol Oxidase/Clay Biosensors

The Determination of Halides and Pseudohalides by the Vanadium Haloperoxidase Based Biosensor

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