Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics

Hackenhaar, Camila Regina; Rosa, Carolina Flores; Flores, Elí Emanuel Esparza; Santagapita, Patricio R.; Klein, Manuela Poletto; Hertz, Plinho Francisco

doi:10.1016/j.carbpol.2022.119483

Cited by 21 publications

(3 citation statements)

References 35 publications

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“…Noteworthy, it was previously reported that both the free and the GA-whey protein isolate grafted Carr beads iβ-GLs manifested their topmost activity at the same pH range 22 . Analogous situations were also recorded after β-GL entrapment within agar 37 and after its covalent binding to tannic acid stabilized silver nano-particles 40 and to genipin grafted alginate-gelatin 41 .…”

Section: Resultsmentioning

confidence: 73%

Glutaraldehyde-pea protein grafted polysaccharide matrices for functioning as covalent immobilizers

Wahba

2023

Sci Rep

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Three polysaccharide matrices (κ-Carrageenan (Carr), gellan gum, and agar) were grafted via glutaraldehyde (GA) and pea protein (PP). The grafted matrices covalently immobilized β-d-galactosidase (β-GL). Nonetheless, grafted Carr acquired the topmost amount of immobilized β-GL (iβ-GL). Thus, its grafting process was honed via Box-Behnken design and was further characterized via FTIR, EDX, and SEM. The optimal GA-PP-Carr grafting comprised processing Carr beads with 10% PP dispersion of pH 1 and 25% GA solution. The optimal GA-PP-Carr beads acquired 11.44 Ug−1 iβ-GL with 45.49% immobilization efficiency. Both free and GA-PP-Carr iβ-GLs manifested their topmost activity at the selfsame temperature and pH. Nonetheless, the β-GL Km and Vmax values were reduced following immobilization. The GA-PP-Carr iβ-GL manifested good operational stability. Moreover, its storage stability was incremented where 91.74% activity was offered after 35 storage days. The GA-PP-Carr iβ-GL was utilized to degrade lactose in whey permeate with 81.90% lactose degradation efficiency.

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Section: Resultsmentioning

confidence: 73%

Glutaraldehyde-pea protein grafted polysaccharide matrices for functioning as covalent immobilizers

Wahba

2023

Sci Rep

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“…There are literature data on another application of enzymes immobilized in gel, particularly their multiple uses in analyses. For example, β-galactosidase was immobilized on a biocomposite of alginate and gelatin crosslinked with genipin, and it maintained 90% relative activity after 11 reuses in a batch process of lactose hydrolysis [68]. The electrode based on glucose oxidase immobilized onto gelatin by cross-linking with chromium III acetate retained more than 60% of its activity after 20 repeated uses [69].…”

Section: Methods Of Producing Preparations Based On Enzymes Immobiliz...mentioning

confidence: 99%

Enzymes Immobilized into Starch- and Gelatin-Based Hydrogels: Properties and Application in Inhibition Assay

Esimbekova,

Torgashina,

Nemtseva

et al. 2023

Micromachines

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The present work is a review of the research on using hydrogels based on natural biodegradable polymers, starch, and gelatin for enzyme immobilization. This review addresses the main properties of starch and gelatin that make them promising materials in biotechnology for producing enzyme preparations stable during use and storage and insensitive to chemical and physical impacts. The authors summarize their achievements in developing the preparations of enzymes immobilized in starch and gelatin gels and assess their activity, stability, and sensitivity for use as biorecognition elements of enzyme inhibition-based biosensors.

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“…Therefore, gelatin needs to be applied to wound dressings through graft modification and chemical and physical cross-linking methods. Oxidized carboxymethyl cellulose (OCMC) as a polysaccharide dialdehyde has the characteristics of rich sources, biocompatibility, and water solubility compared to traditional gelatin cross-linking agents such as genipin and glutaraldehyde . Lei et al employed OCMC as a natural macromolecular cross-linking agent to form pH-responsive hydrogels with gelatin through a Schiff base reaction.…”

Section: Introductionmentioning

confidence: 99%

Multicomponent Synergistic Antibacterial Hydrogel Based on Gelatin-Oxidized Carboxymethyl Cellulose for Wound Healing of Drug-Resistant Chronic Infection

Zhang,

Wang,

Wang

et al. 2024

ACS Appl. Bio Mater.

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Bacterial invasion hinders the healing process of wound, leading to the formation of chronic infected wound; meanwhile, the misuse of antibiotics has resulted in the emergence of numerous drug-resistant bacteria. The application of conventional antimicrobial methods and wound treatment techniques is not appropriate for wound dressings. In this paper, quaternized poly(vinyl alcohol) (QPVA) and pomegranate-like copper uniformly doped polydopamine nanoparticles (PDA@Cu) were introduced into a gelatin-oxidized carboxymethyl cellulose system to form a multicomponent synergistic antibacterial hydrogel (GOQ 3 P 3 ). Polydopamine improves the biocompatibility and prevents the detachment of Cu nanoparticles. It can achieve synergistic antibacterial effects through quaternary ammonium salt-inorganic nanoparticle photothermal treatment under 808 nm near-infrared (NIR) irradiation. It exhibits highly efficient and rapid bactericidal properties against Escherichia coli, Staphylococcus aureus, and MRSA (methicillin-resistant Staphylococcus aureus) with an antibacterial rate close to 100%. The gel scaffold composed of macromolecules gives the hydrogel excellent mechanical properties, adhesive capabilities, self-healing characteristics, biocompatibility, and pH degradation and promotes cell adhesion and migration. In a full-thickness wound healing model infected with MRSA, GOQ 3 P 3 controls inflammatory responses, accelerates collagen deposition, promotes angiogenesis, and enhances wound closure in the wound healing cascade reaction. This study provides a feasible strategy for constructing dressings targeting chronic infection wounds caused by drug-resistant bacteria.

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Development of a biocomposite based on alginate/gelatin crosslinked with genipin for β-galactosidase immobilization: Performance and characteristics

Cited by 21 publications

References 35 publications

Glutaraldehyde-pea protein grafted polysaccharide matrices for functioning as covalent immobilizers

Glutaraldehyde-pea protein grafted polysaccharide matrices for functioning as covalent immobilizers

Enzymes Immobilized into Starch- and Gelatin-Based Hydrogels: Properties and Application in Inhibition Assay

Multicomponent Synergistic Antibacterial Hydrogel Based on Gelatin-Oxidized Carboxymethyl Cellulose for Wound Healing of Drug-Resistant Chronic Infection

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