Development of a bioassay for putative human lymphoma stem cells

Jones, Stephen E.; Hamburger, Anne W.; Kim, Mary B.; Salmon, Sydney E.

doi:10.1182/blood.v53.2.294.294

Cited by 31 publications

(1 citation statement)

References 9 publications

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“…In all cases, cloning efficiency was low, ranging from 0.0003 to 0.04% ( Table 11). In contrast, these latter populations can be selectively induced to clonal growth by certain environmental conditions, eg, relative hypoxia [2], or the presence of conditioned media (CM) [5]. CM from human Blymphocyte tissue culture lines seem to be most effective for the induction of lymphoid colonies from tissues involved by non-Hodgkin lymphomas.…”

Section: H-tdr Labeling Index Studiesmentioning

confidence: 99%

Cloning of fresh lymphoma cells

Nishikori

Hansen

Yen

et al. 1981

Med. Pediatr. Oncol.

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We attempted to induce in vivo clonal growth of neoplastic lymphoid cells from fresh specimens of involved tissue from 22 patients with no-Hodgkin lymphoma (NHL). Conditioned media derived from two human B-lymphocyte tissue culture lines were tested for their ability to promote colony growth. In addition, we compared the incidence of colony induction to the flash-3H-thymidine labeling index (LI) of the cells in the tissues cultured. Successful colony induction occurred in three-tenths of the cases of diffuse histiocytic lymphoma and one-half of the cases of nodular lymphoma. Cloning efficiencies were low, ranging from 0.0003 to 0.04%. There was no apparent relationship between successful instances of cloning and LI. We have confirmed the observations of Jones et al that lymphoid tumor cells can be cloned from tissues involved by NHL using a soft agar system. The possibility of using such systems for clinical predictive assays of chemotherapeutic drug toxicity toward tumor cells of NHL is discussed. We conclude that further refinements of the assays are desirable before they can be applied to widespread clinical use.

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Section: H-tdr Labeling Index Studiesmentioning

confidence: 99%

Cloning of fresh lymphoma cells

Nishikori

Hansen

Yen

et al. 1981

Med. Pediatr. Oncol.

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Application of in vitro soft agar techniques for growth of tumor cells to the study of colon cancer

Buick

Fry

Salmon

1980

Cancer

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An in vitro assay to measure the clonogenic or colony-forming capability of cancer cells present in biopsy samples has recently been applied to study the biology and drug-sensitivity of a variety of human neoplasms. This approach appears to be suitable for study of the tumor stem or progenitor cells present in malignant effusions from patients with colonic carcinoma. In our preliminary studies, morphology of the tumor colonies by inverted microscopy and with Papanicolaou staining of dried agar plating layers as well as immunofluorescent localization with a specific antiserum to human carcinoembrionic antigen have been used as markers of the neoplastic origin of colon tumor colony-forming cells. Successful application of this assay to colonic solid tumors will require improvement in techniques for disaggregation of viable clonogenic cells. We anticipate that short term clonal assays will have increasing use for clinical and biological studies of human colon cancer.

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In vitro growth of lymphoma colonies from children with non-hodgkin's lymphoma

Smith

Wood

Fried

et al. 1981

Cancer

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Growth in vitro of malignant lymphoid cells has been previously reported, but those studies involved direct establishment of cell lines from the patients' cells. In the present study, an in vitro system for both primary growth and passage of malignant lymphoblastic colony-forming cells (ML-CFC) was established, and for the first time, colony-forming cells from non-Hodgkin's lymphoma patients were cultured, and cell lines were established from individual colonies. Malignant lymphoid colonies grew from 9 of 16 (56%) bone marrow aspirates, which were histologically involved with lymphoma and from 5 of 29 (17%) morphologically normal bone marrow samples. ML-CFCs grew concomitantly with myeloid and monocytic CFCs and were distinguishable by their morphologic characteristics on agar, cytochemical staining characteristics, and cell surface markers. Successful passage of ML-CFCs was accomplished in five patients, and ten distinct cell lines (six null cell, three B cell, and one T cell) were established. This agar assay system provides an opportunity to study malignant lymphoma cell growth both as a primary colony and a cell line established from a primary colony.

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Development of a bioassay for putative human lymphoma stem cells

Cited by 31 publications

References 9 publications

Cloning of fresh lymphoma cells

Cloning of fresh lymphoma cells

Application of in vitro soft agar techniques for growth of tumor cells to the study of colon cancer

In vitro growth of lymphoma colonies from children with non-hodgkin's lymphoma

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