Development of a 99mTc-labeled lactam bridge-cyclized alpha-MSH derivative peptide as a possible single photon imaging agent for melanoma tumors

Shamshirian, Danial; Erfani, Mostafa; Beiki, Davood; Fallahi, Babak; Shafiei, Mohammad

doi:10.1007/s12149-015-0998-y

Cited by 6 publications

(2 citation statements)

References 42 publications

(68 reference statements)

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“…Synthesizing the cyclic pentapeptide with cyclo(D-Tyr1-D-Orn2-Arg3-2-Nal4-Gly5) sequence was in accordance with our previous reports (Mikaeili et al 2017(Mikaeili et al ,2018Shamshirian et al 2015). The synthesis procedure and the chemical structure of cyclic peptide conjugated with HYNIC have been showed in Fig.…”

Section: Methodssupporting

confidence: 84%

“…Peptide radiolabeling was achieved in accordance with previous reports (Mikaeili et al 2017;Shamshirian et al 2015) in which peptide (5 µg), tricine (15 mg) and EDDA (5 mg) dissolved in water (0.5 mL) and mixed with solution of stannous chloride dihydrate (in 0.1 M HCl; 2 mg/mL; 20 µL). Finally, [ 99m Tc]technetium pertechnetate (740 MBq) in saline (0.5 mL) was added to the peptide solution and heated (100 °C) for 10 min.…”

Section: Tc-labeling Of Hynic-cyclic Pentapeptide and Radiochemical Analysismentioning

confidence: 99%

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Breast Tumor Targeting in Mice Bearing 4T1 Tumor with Labeled CXCR4 Antagonist Analogue

Mikaeili¹,

Erfani²,

Goudarzi³

et al. 2021

Int J Pept Res Ther

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Chemokine receptor belongs to G-protein-coupled cell-surface receptors. CXCR4 as a chemokine receptor is over-expressed in many type of solid tumors and metastasis in which breast tumor is one of them. A CXCR4 targeted peptide was prepared and labeled with 99m Tc. Subsequently, receptor binding internalization, in vivo tumor uptake and biodistribution were assessed. The receptor binding internalization rate was evaluated using 4T1 breast tumor cells. BALB/c female mice bearing 4T1 tumors were employed for investigation of radioconjugate biodistribution. Radioconjugate was obtained with labeling yield of > 95%. The highest achieved specific activity was 123 MBq/nmol. The in vitro cell uptake test resulted specific internalization into 4T1 cells (average of 1.90 ± 0.12% of total add at 2 h). Animal biodistribution data showed tumor uptake of 1.18 ± 0.11% ID/g after 30 min post injection. Our findings demonstrated that the labeled peptide can be a suitable candidate for breast tumors imaging.

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Section: Methodssupporting

confidence: 84%

Section: Tc-labeling Of Hynic-cyclic Pentapeptide and Radiochemical Analysismentioning

confidence: 99%

Breast Tumor Targeting in Mice Bearing 4T1 Tumor with Labeled CXCR4 Antagonist Analogue

Mikaeili¹,

Erfani²,

Goudarzi³

et al. 2021

Int J Pept Res Ther

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Synthesis and labeling of p-NH2-Bn-DTPA-(Dabcyl-Lys6,Phe7)-pHBSP with 99mTc as a radiopeptide scintigraphic agent to detect cardiac ischemia

Mohtavinejad

Bitarafan-Rajabi

Ardestani

et al. 2020

J Radioanal Nucl Chem

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Al18F-labeled alpha-melanocyte-stimulating hormone (α-MSH) peptide derivative for the early detection of melanoma

et al. 2019

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Development of a 99mTc-labeled lactam bridge-cyclized alpha-MSH derivative peptide as a possible single photon imaging agent for melanoma tumors

Abstract: The obtained results show that the presented new designed labeled peptide conjugate may be a suitable candidate for diagnosis of malignant tumors.

Cited by 6 publications

References 42 publications

Breast Tumor Targeting in Mice Bearing 4T1 Tumor with Labeled CXCR4 Antagonist Analogue

Breast Tumor Targeting in Mice Bearing 4T1 Tumor with Labeled CXCR4 Antagonist Analogue

Synthesis and labeling of p-NH2-Bn-DTPA-(Dabcyl-Lys6,Phe7)-pHBSP with 99mTc as a radiopeptide scintigraphic agent to detect cardiac ischemia

Al18F-labeled alpha-melanocyte-stimulating hormone (α-MSH) peptide derivative for the early detection of melanoma

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