Development of a 3D porous chitosan/gelatin liver scaffold for a bioartificial liver device

Hou, Yung-Te; Hsu, Chao-Chun

doi:10.1016/j.jbiosc.2019.12.012

Cited by 21 publications

(9 citation statements)

References 57 publications

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“…Thus, several morphological variations exist in liver organoids, probably depending on the degree of cell differentiation; however, branching morphogenesis seems obscure 53 . When evaluating hepatocytotoxicity in vitro , the most common method is to collect whole treated cells and detect CYP induction, cell death, and cell proliferation while checking the degree of hepatocyte differentiation (albumin and urea production) 31 , 32 , 61 , 63 . However, in the case of organoids, it may be possible to detect more detailed toxic reactions by histopathologically confirming the type of organoid ( Fig.…”

Section: Proposed Histopathological Organoid Observationmentioning

confidence: 99%

The potential of organoids in toxicologic pathology: role of toxicologic pathologists in <i>in vitro</i> chemical hepatotoxicity assessment

et al. 2022

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The development of in vitro toxicity assessment methods using cultured cells has gained popularity for promoting animal welfare in animal experiments. Herein, we briefly discuss the current status of hepatoxicity assessment using human- and rat-derived hepatocytes; we focus on the liver organoid method, which has been extensively studied in recent years, and discuss how toxicologic pathologists can use their knowledge and experience to contribute to the development of in vitro chemical hepatotoxicity assessment methods for drugs, pesticides, and chemicals. We also propose how toxicological pathologists should assess toxicity regarding the putative distribution of undifferentiated and differentiated cells in the organoid when liver organoids are observed in hematoxylin and eosin–stained specimens. This was done while considering the usefulness and limitations of in vitro studies for toxicologic pathology assessment.

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Section: Proposed Histopathological Organoid Observationmentioning

confidence: 99%

The potential of organoids in toxicologic pathology: role of toxicologic pathologists in <i>in vitro</i> chemical hepatotoxicity assessment

et al. 2022

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“…For biodegradability, both the NPT (n = 6) and DPT (n = 6) were subjected to 1XPBS (pH ~ 7.4) immersion containing 0.2% w/v collagenase (from Clostridium histolyticum, Sigma-Aldrich-SCR103) and incubated in the shaker incubator at 37°C. 46 The tissue was weighed every 4 h until it was completely degraded while submerged in the enzymatic solution. The initial weight was calculated by taking the native tissue before immersing it in the enzymatic concoction, and later it was weighed at different time checkpoints until it was intact.…”

Section: Biodegradability Testmentioning

confidence: 99%

Establishment of decellularized extracellular matrix scaffold derived from caprine pancreas as a novel alternative template over porcine pancreatic scaffold for prospective biomedical application

et al. 2022

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In this study, the caprine pancreas has been presented as an alternative to the porcine organ for pancreatic xenotransplantation with lesser risk factors. The obtained caprine pancreas underwent a systematic cycle of detergent perfusion for decellularization. It was perfused using anionic (0.5% w/v sodium dodecyl sulfate) as well as non‐ionic (0.1% v/v triton X‐100, t‐octyl phenoxy polyethoxy ethanol) detergents and washed intermittently with 1XPBS supplemented with 0.1% v/v antibiotic and nucleases in a gravitation‐driven set‐up. After 48 h, a white decellularized pancreas was obtained, and its extracellular matrix (ECM) content was examined for scaffold‐like properties. The ECM content was assessed for removal of cellular content, and nuclear material was evaluated with temporal H&E staining. Quantified DNA was found to be present in a negligible amount in the resultant decellularized pancreas tissue (DPT), thus prohibiting it from triggering any immunogenicity. Collagen and fibronectin were confirmed to be preserved upon trichrome and immunohistochemical staining, respectively. SEM and AFM images reveal interconnected collagen fibril networks in the DPT, confirming that collagen was unaffected. sGAG was visualized using Prussian blue staining and quantified with DMMB assay, where DPT has effectively retained this ECM component. Uniaxial tensile analysis revealed that DPT possesses better elasticity than NPT (native pancreatic tissue). Physical parameters like tensile strength, stiffness, biodegradation, and swelling index were retained in the DPT with negligible loss. The cytocompatibility analysis of DPT has shown no cytotoxic effect for up to 72 h on normal insulin‐producing cells (MIN‐6) and cancerous glioblastoma (LN229) cells in vitro. The scaffold was recellularized using isolated mouse islets, which have established in vitro cell proliferation for up to 9 days. The scaffold received at the end of the decellularization cycle was found to be non‐toxic to the cells, retained biological and physical properties of the native ECM, suitable for recellularization, and can be used as a safer and better alternative as a transplantable organ from a xenogeneic source.

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“…A three−dimensional liver scaffold was fabricated from a chitosan/gelatin (CG) solution cross−linked with glutaraldehyde and showed a porous structure similar to the extracellular matrix that facilitated hepatocyte adhesion and proliferation; this CG scaffold had high hepatocyte biocompatibility and mechanical strength but also maintained hepatic functions and viability in in vitro cultures; especially, this liver scaffold revealed high potential for further bioartificial liver design in the near future [ 179 ].…”

Section: Asas With Apsmentioning

confidence: 99%

Antimicrobial Polymer−Based Assemblies: A Review

Carmona-Ribeiro

Araújo

2021

IJMS

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An antimicrobial supramolecular assembly (ASA) is conspicuous in biomedical applications. Among the alternatives to overcome microbial resistance to antibiotics and drugs, ASAs, including antimicrobial peptides (AMPs) and polymers (APs), provide formulations with optimal antimicrobial activity and acceptable toxicity. AMPs and APs have been delivered by a variety of carriers such as nanoparticles, coatings, multilayers, hydrogels, liposomes, nanodisks, lyotropic lipid phases, nanostructured lipid carriers, etc. They have similar mechanisms of action involving adsorption to the cell wall, penetration across the cell membrane, and microbe lysis. APs, however, offer the advantage of cheap synthetic procedures, chemical stability, and improved adsorption (due to multipoint attachment to microbes), as compared to the expensive synthetic routes, poor yield, and subpar in vivo stability seen in AMPs. We review recent advances in polymer−based antimicrobial assemblies involving AMPs and APs.

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Development of a 3D porous chitosan/gelatin liver scaffold for a bioartificial liver device

Cited by 21 publications

References 57 publications

The potential of organoids in toxicologic pathology: role of toxicologic pathologists in <i>in vitro</i> chemical hepatotoxicity assessment

The potential of organoids in toxicologic pathology: role of toxicologic pathologists in <i>in vitro</i> chemical hepatotoxicity assessment

Establishment of decellularized extracellular matrix scaffold derived from caprine pancreas as a novel alternative template over porcine pancreatic scaffold for prospective biomedical application

Antimicrobial Polymer−Based Assemblies: A Review

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